Update Your ProfileAcademic Title:
Adjunct Associate Professor
Primary Appointment:
Medicine
Location:
HH 445A, labs 421/427/430
Phone (Primary):
(410)-7064047
Education and Training
1980 BS Biochemistry, Univ. of Sciences, Paris VII, France
1981 MS Cell Physiology, Univ. of Sciences, Paris VII, France
1981 MS Human Biology, Univ. of Sciences, Paris VII, France
1984 PhD Nutrition, Univ. of Sciences, Paris VII, France
Post Graduate Education and Training
1985-1986 Post-doctoral Associate, Laboratory of Human Behavior and Metabolism. The RockefellerUniv., NY, Profs. Jules Hirsh and Irving Faust, Mentors
1986-1987 Adjunct Assist. Professor, Department of Biology, Vassar College, NY, Professors. Marcie Greenwood and Irving Faust, Mentors
1988-1992 Research Associate, VAH Palo Alto, Division of Endocrinology, Stanford, University School of Medicine, Stanford, CA, Prof. Gerald Reaven
1993-1997 Research Associate, VAH Palo Alto, Division of Endocrinology, Stanford University School of Medicine, Stanford, CA, Prof. Fredrick Kraemer
1998-2003 Research Fellow, NIDDK, Bethesda, MD, Dr. Constantine Londos Advisor
Biosketch
Professional Experience
2003-present VA Research Physiologist, VAMC under the supervision of Dr. Andrew Goldberg (until 2014) then Dr. Leslie Katz.
2003-2009 Assistant Professor, Department of Medicine, Division of Gerontology, University of MarylandSchool of Medicine
2009-present Associate Professor, Department of Medicine, Division of Endocrinology. University of MarylandSchool of Medicine
2007-2009 Adipose Core manager (NORC)
2009-present Co-Director Biological Mechanisms Core Laboratory (NIH Mid-Atlantic Nutrition Obesity Research Center (NORC))
Research/Clinical Keywords
Obesity, diabetes, metabolic complications of obesity, adipose tissue, fatty liver, lipid metabolism, lipid droplet
Highlighted Publications
- Bell M., Wang H., Chen H, McLenithan J., Yang R, Yu D., Fried S.K., Quon M.J., Londos C. and Sztalryd C. Consequences of lipid droplet coat proteins down –regulation in liver cells: Abnormal lipid droplet metabolism and induction of insulin resistance. Diabetes 2008 57(8):2037-45.
- Beller M, Sztalryd C, Southall N, Bell M, Jäckle H, Auld DS, Oliver B. COPI complex is a regulator of lipid homeostasis. PLoS Biol. 2008 6(11) e29
- Kimmel A.R., Brasaemle D.L., McAndrews-Hill M., Sztalryd C., Londos C.. Adoption of.PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular, lipid storage droplet proteins. J Lipid Res. 2009 Jul 28. [Epub ahead of print]
- Wang H., Hu L., Dalen K., Dorward H., Marcinkiewicz A., Russel D., Gong D., Londos C.,Yamaguchi T., Holm C., Rizzo M.A., Brasaemle D., Sztalryd C. Activation of hormone-sensitive lipase requires two steps: protein phosphorylation and binding to the PAT-1 domain of lipid droplet coat proteins. J Biol Chem. 2009 284 (46):32116-321125
- Wang H. and Sztalryd C. Oxidative tissue: perilipin 5 links storage with the furnace. Trends in Endocrinology. 2011. 22(6):197-203.
- Wang H, Sreenevasan U, Hu H, Saladino A, Polster BM, Lund LM, Gong DW, Stanley WC, Sztalryd C. Perilipin 5, lipid droplet associated protein provides physical and metabolic linkage to mitochondria. J Lipid Res. 2011. 52(12):2159-2168.
- Wang X, Yang R, Jadhao SB, Yu D, Hu H, Glynn-Cunningham N, Sztalryd C, Silver KD, Gong DW.Transmembrane emp24 protein transport domain 6 is selectively expressed in pancreatic islets and implicated in insulin secretion and diabetes.. Pancreas. 2012.41(1):10-14.
- Konige M, Wang H, Sztalryd C. Role of adipose specific lipid droplet proteins in maintaining whole body energy homeostasis. Biochim Biophys Acta. 2013 1842(3):393-401
- Wang H, Sreenivasan U, Gong DW, O'Connell KA, Dabkowski ER, Hecker PA, Ionica N, Konig M, Mahurkar A, Sun Y, Stanley WC, Sztalryd C. Cardiomyocyte-specific perilipin 5 overexpression leads to myocardial steatosis and modest cardiac dysfunction. J Lipid Res. 2013 Apr;54(4):953-65.
- Sztalryd C, Kimmel AR. Perilipins: Lipid droplet coat proteins adapted for tissue-specific energy storage and utilization, and lipid cytoprotection. Biochimie. 2013 Jan;96:96-101
- Albert, JS, Yerges-Armstrong, LM, Horenstein, RB, Pollin, TI, Sreenivasan, UT, Chai S, Blaner, WS, Snitker, S, O'Connell, JR, Gong D-W, Breyer III, RJ, Ryan, AS, McLenithan, JC, Shuldiner, AR, Sztalryd, C*, Damcott, CM*..Null Mutation in Hormone-Sensitive Lipase Gene and Risk of Type 2 Diabetes. New England Journal of Medicine, N Engl J Med. 2014 Jun 12;370(24):2307-15. * Authors have equally contributed to the work.
- Tschapalda K, Zhang Y-Q, Liu Li, Golovnina K, Schlemper T,. Eichmann T O, Lal-Nag M, Sreenivasan U, McLenithan J, Ziegler S, Sztalryd C, Achim L, Auld D, Oliver B, Waldmann H, Li Zhuyin, Shen M, Boxer M B., Beller. A novel class of diacylglycerol acyltransferase 1 inhibitors identified by a combination of phenotypic high-throughput screening, genomics, and genetics EBioMedicine, 2016 Jun;8:49-59.
- Kimmel A, Sztalryd C. The perilipins: Major cytosolic lipid droplet-associated proteins and their roles in cellular lipid storage mobilization, and systemic homeostasis. Annu. Rev. Nutr. Annu Rev Nutr. 2016 Jul 17;36:471-509.
Additional Publication Citations
Research Interests
My scientific interest has been to determine mechanisms underlying association of obesity, a chronic positive energy balance, and development of metabolic diseases, e.g. Type 2 Diabetes. I built a research program at UMB focused on understanding contributions of cytosolic lipid droplets (CLD) to cellular and systemic lipid homeostasis in both physiological and pathological conditions, with funding support from NIH, American Diabetes Association (ADA) and American Heart Association (AHA). Key questions addressed in my laboratory are the following: what gene products regulate CLD degradation and their tissue specificity; how and what links CLD in adipose, liver, and muscle with metabolic disease? My work has contributed significantly to understanding how perilipin protein family members regulate tissue specific CLD hydrolysis via each member’s ability to protect the CLD neutral lipid core from key lipolytic enzymes. My work also helped develop a concept that ectopic CLD are an adaptive response to changes in lipid homeostasis, and not necessarily pathologic. Rather, defects in CLD regulation and metabolism can lead to disease. My lab learned to use perilipin proteins as effective discovery tools to study CLD function, and we developed important tools and methodologies for the field to study CLD biology and lipid metabolism in cell culture systems and tissues.
As the Director of the Biological Mechanisms Core Laboratory (NIH Mid-Atlantic Nutrition Obesity Research Center (NORC)), I developed strong interactions with the entire NORC scientific community, and in particular members involved in studying the genetics of complex disorders in the Old Order Amish including T2D, insulin resistance obesity and cardiovascular diseases (CVD). An example of this is illustrated by a major contribution made to elucidate the molecular mechanisms underlying a novel 19 base pair deletion that disrupts the coding region of the LIPE gene in Amish subjects with dyslipidemia. LIPE encodes hormone sensitive lipase (HSL), a key lipolytic enzyme, recently published in New England Journal of Medicine (with two press releases), and reviewed in Cell Metabolism. The accelerating pace of genomic discovery among NORC users has increased the need for mechanistic studies to elucidate the physiological roles of novel genes in cells, tissues, and whole organisms. To achieve this goal, with the help of my Co-Director Dr. Zaghloul, we have developed three types of services: i) adipose tissue phenotyping and tissue/cell banking; ii) whole animal functional genomics (zebrafish genetic models) and iii) functional genomics in tissue culture cells (including over-expression and knockdown in cultured cells, analytical and metabolic assays, and confocal imaging). Combining my expertise in CLD biology, lipid metabolism, and molecular and imaging technologies with Dr. Zaghoul expertise in zebrafish system, we are now evaluating the impact of TM6SF2 variants in human triglyceride-rich lipoprotein secretion. Our on-going takes advantage of a unique ‘genetic experiment of nature’ which offers opportunities to gain molecular insights in human regulation of TRL secretion, a pathway associated with unmet medical needs.
Awards and Affiliations
Honors and Awards
1981-1982 Grant from the Univ. of Paris
1982-1982 Grant from the French Ministry of Research
1984-1986 Grant from the French Foundation for Nutrition Research
1986-1987 Feasibility Grant from the ObesityCoreCenter (NIH)
1989-1991 Grant from Weight Watchers
1993-1995 Grant from State of CA, Effect of Nicotine on Weight Gain
1995-1996 Grant in Aid, CA Amer. Heart Association, Insulin Regulation of LPL
2005-2010 ADA Career Development Award 1-05 CD-`17. Perilipin regulation of HSL
2014 best publication award of the department of Medicine
Professional Activity
Institutional Service
2005-2008 Adipocyte Core Biology Manager, UMSOM
2008-present Adipocyte Core Biology, Director UMSOM
2006-2012 Member of the Department of Gerontology and GRECC Biosafety Committee
Review and provide guidelines for Biosafety protocols in use in the GRECC, VAMC
2007-present Ad Hoc Grant reviewer for CNRU pilot feasibility grants, UMSOM
2008-present University of Maryland School of Medicine Council Representative (alternate)
2008-2014 VA grant review subcommittee, VAMC
2008-2012School of Medicine council member
Local and National Service
2003-2009 Special volunteer, NIDDK/LCDB
2004-present Ad Hoc Reviewer (Diabetes, J. Cell Biology, J..Lipid Research, Nature commons, ,Am. J. of Clin. Nutrition, Obesity, European J. of Physiology, J.Mol. Medicine, In Vitro Cellular and Development Biology,BBA lipids, Diabetologia, PNAS,Science reports, Biochmistry, Journal of Biological Chemistry, PNAS, Am. J. of Physiology, Endocrinology, FEBBS, FASEB Journal, , Traffic, Biochemistry, Obesity J.)
2006-2010 Grant reviewer for NIH seeds grant, VillaJulieCollege
2006-present Grant reviewer for NIH funded Nutrition, Obesity and Research Center seeds grant
2007-2008 Grant reviewer for American Heart Association, national chapter: lipids study section
2008-2013 Ad Hoc Grant reviewer for American Heart Association, Mid-Atlantic chapter: lipids study section
2007-2015 Grant reviewer for American Diabetes Association Research Review Grant Committee
2016-present NIDDK Diabetes COMP reviewer
2011-present ad Hoc reviewer for welcome trust
2011-present maryland and Stanford DRC Pilot & Feasibility Grant Reviewer
2005-present Maryland U NORC Pilot & Feasibility Grant reviewer
2007-present Member of the editorial board of the Journal of Obesity (Nature Publishing Group)
2008-present Ad Hoc Grant reviewer for NIH CADO, IPOD, NSF, Wellcome Trust, British Diabetes Association study sections
2011 American Diabetes Association, Chair, Lipid droplet session
2013 Gordon conference, discussion leader, Lipid droplet session
2013 Co-organizer of the Society for Heart and Vascular Metabolism “lipids in cardiac health and diseases: from toxicity and protection, Cambridge, Maryland.
2014 FASEB summer meetings. Chair, Lipid Droplet session
Lab Techniques and Equipment
Unltracentrifuge bexkamn and rotors
French press
in vitro metabolic labeling
Adipose tissues phenotyping