Shengbing Wang, PhD

1993 - 1997     B.S., Analytical Chemistry, University of Science and Technology of Changchun (Changchun, China)            

1997 - 2000     M.S., Cellular and Molecular Biology, University of Science and Technology of China (Hefei, China)

2000 - 2004     Ph.D., Biochemistry, University of Hong Kong (Hong Kong, China), Thesis Advisor – Feng Chen,   “A Proteomic Study of the Green Alga Haematococcus pluvialis” 

 I have a broad background in protein biochemistry, molecular biology and cardiology, with specific training and expertise in oxidative posttranslational modifications and phosphorylation/kinase signaling network research and state-of-art proteomics tools to dissect the molecular mechanism of cardiovascular disease.  My research includes redox regulation of ATP synthase in the development of heart failure and its clinical treatment.  As co-Investigator on several NIH-funded grants, I have developed new techniques for quantitative analysis of S-nitrosylation and site-specific phosphorylation in heart disease and I have been a primary contributor in obtaining preliminary data for the present proposal. In addition, I successfully developed a functional pipeline to study the function of the site-specific posttranslational modifications in cell culture systems and produced several peer-reviewed publications from these projects.  As a result of this previous experience, I am aware of the importance of frequent communication among project members and of constructing a realistic research plan, timeline, and budget.  The current application builds logically on my prior work and I look forward to the exciting possibilities for protein purification afforded by epitope-tagging native proteins using CRISPR/Cas9, which will revolutionize our capabilities when combined with stat-of-the-art mass spectrometry techniques.

Proteomics, protein posttranslational modification, birth defect