Amanda G. Oglesby-Sherrouse, PhD

1996 - 2000, B.S. in Microbiology, University of Texas at Austin

2000 - 2005, Ph.D. in Microbiology, University of Texas at Austin

2006 - 2012, Post-doctoral Fellow, University of Colorado School of Medicine  

Iron is an essential metallonutrient for nearly all living organisms and represents a primary driver of host-pathogen interactions. During infection, pathogenic bacteria must be able to manage their requirement for iron with its potential for toxicity. This is achieved through a combination of iron uptake and regulatory pathways, which function to maintain iron homeostasis. The focus of our laboratory’s research is to understand how these systems mediate iron homeostasis and contribute to bacterial pathogenesis. Our studies are currently focused on Pseudomonas aeruginosa and Staphylococcus aureus, opportunistic pathogens of significant concern for hospital-acquired infections and in cystic fibrosis patients. We use a multidisciplinary approach to determine how iron homeostasis is mediated by these pathogens, as well as the impact of these systems on pathogenesis.

For more information, visit my Lab Website.

iron, bacterial pathogenesis, Pseudomonas aeruginosa, gene regulation

Zygiel EM, Nelson CE, Brewer LK, Oglesby-Sherrouse AG, Nolan EM. (2019) The human innate immune protein calprotectin induces iron starvation responses in Pseudomonas aeruginosa. Journal of Biological Chemistry. 294(10) 3549-3562.

Nelson CE, Huang W, Brewer LK, Nguyen AT, Kane MA, Wilks A, Oglesby-Sherrouse AG. (2018) Proteomic analysis of the Pseudomonas aeruginosa iron starvation response reveals PrrF sRNA-dependent regulation of amino acid metabolism, iron-sulfur cluster biogenesis, motility, and zinc homeostasis proteins. BioRxiv 477984. Preprint.

Djapgne L, Panja S, Brewer LK, Gans J, Kane MA, Woodson SA, Oglesby-Sherrouse AG. (2018) The Pseudomonas aeruginosa PrrF1 and PrrF2 small regulatory RNAs (sRNAs) promote 2-alkyl-4-quinolone production through redundant regulation of the antR mRNA. Journal of Bacteriology. 200(10) e00704-17.

Gans J, Osborne J, Cheng J, Djapgne L, Oglesby-Sherrouse AG. (2018) Sequence-specific affinity chromatography of bacterial small regulatory RNA-binding proteins from bacterial cells. Methods in Molecular Biology 1737:341-350.

Little AS, Okkotsu Y, Reinhart AA, Damron FH, Barbier M, Barrett B, Oglesby-Sherrouse AG, Goldberg JB, Cody WL, Schurr MJ, Vasil ML, Schurr MJ. (2018) Pseudomonas aeruginosa AlgR phosphorylation status differentially regulates pyocyanin and pyoverdine production. mBio 9(1) e02318-17.

Huang W, Brewer LK, Jones JW, Nguyen AT, Marcu A, Wishart DS, Oglesby-Sherrouse AG, Kane MA, Wilks A. (2018) PAMDB: a comprehensive Pseudomonas aeruginosa metabolome database. Nucleic Acids Research 46(D1) D575–D580.

Reinhart AA, Nguyen AT, Brewer LK, Bevere J, Jones JW, Kane MA, Damron FH, Barbier M, and Oglesby-Sherrouse AG (2017) The Pseudomonas aeruginosa PrrF small RNAs regulate iron homeostasis during acute murine lung infection. Infection and Immunity 85(5):e00764-16.

Damron FH, Oglesby-Sherrouse AG, Wilks A, Barbier M. (2016) Dual-seq transcriptomics reveals the battle for iron during Pseudomonas aeruginosa acute murine pneumonia. Scientific Reports 6: 39172.

Nguyen AT, Jones JW, Camara M, Williams P, Kane MA, Oglesby-Sherrouse AG. (2016). Cystic fibrosis isolates of Pseudomonas aeruginosa retain iron-regulated antimicrobial activity against Staphylococcus aureus through the action of multiple alkylquinolones. Frontiers in Microbiology 7:1171.

Nguyen AT and Oglesby-Sherrouse AG. (2016). Interactions between Pseudomonas aeruginosa and Staphylococcus aureus during co-cultivations and polymicrobial infections. Applied Microbiology and Biotechnology 100(14):6141-6148. Review

Reinart AA and Oglesby-Sherrouse AG. (2016) Regulation of Pseudomonas aeruginosavirulence by distinct iron sources. Genes 7(12):126.

Nguyen AT and Oglesby-Sherrouse AG. (2015). Spoils of war: iron at the crux of clinical and ecological fitness of Pseudomonas aeruginosa. Biometals 28(3):433-443. Review.

Nguyen AT, Jones JW, Ruge MA, Kane MA, and Oglesby-Sherrouse AG. (2015). Iron depletion enhances production of antimicrobials by Pseudomonas aeruginosa. Journal of Bacteriology 197(14):2265-75.

Reinhart AA, Powell DA, Nguyen AT, O’Neill M, Djapgne L, Wilks A, Ernst RK, Oglesby-Sherrouse AG. (2015) The prrF-encoded small regulatory RNAs are required for iron homeostasis and virulence of Pseudomonas aeruginosa. Infection and Immunity 83(3):863-875.

Nguyen AT, O’Neill M, Watts AM, Robson CL, Lamont IL, Wilks A, Oglesby-Sherrouse AG.(2014) Adaptation of iron homeostasis pathways by a Pseudomonas aeruginosapyoverdine mutant in the cystic fibrosis lung. Journal of Bacteriology 196(12):2265-76.

Osborne J, Djapgne L, Tran B, Goo YA, Oglesby-Sherrouse AG. (2014) A method for in vivo identification of bacterial small RNA binding proteins. Microbiology Open 3(6):950-60.

Caswell CC, Oglesby-Sherrouse AG, Murphy ER. (2014). Sibling rivalry: related bacterial small RNAs and their redundant and non-redundant roles. Frontiers in Cellular and Infection Microbiology 4:151. Review.

Oglesby-Sherrouse AG, Djapgne L, Nguyen AT, Vasil AI, Vasil ML. (2014) The complex interplay of iron, biofilm formation, and mucoidy affecting antimicrobial resistance of Pseudomonas aeruginosa. Pathogens and Disease 70(3):307-20.

Oglesby-Sherrouse AG and Murphy ER. (2013) Iron-Responsive Bacterial Small RNAs: Variations on a Theme. Metallomics 5(4):276-286. Review. Cover image.

Oglesby-Sherrouse AG, Vasil ML. (2010) Characterization of a heme-regulated non-coding RNA encoded by the prrF locus of Pseudomonas aeruginosa. PLoS One 5(4):e9930.

Oglesby AG, Farrow JM 3rd, Lee JH, Tomaras AP, Greenberg EP, Pesci EC, Vasil ML. (2008) The influence of iron on Pseudomonas aeruginosa physiology: a regulatory link between iron and quorum sensing. Journal of Biological Chemistry 283(23):15558-67.

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