Update Your ProfileAcademic Title:
Professor
Primary Appointment:
Neurobiology
Secondary Appointment(s):
Psychiatry
Administrative Title:
Associate Director, Basic & Translational Science at the Kahlert Institute of Addiction Medicine
Email:
Location:
HSF II, S265
Phone (Primary):
(410) 706-8824
Education and Training
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1998 B.S., Biology and Minor in Anthropology, University of California Los Angeles
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2007 Ph.D., Neuroscience, University of California Los Angeles- Thesis Advisor- X. William Yang, M.D, Ph.D., “Genetic Analyses of Striatal Projection Neuron Subtypes in the Direct and Indirect Pathways”
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2007-2008 Postdoctoral Fellow, Laboratory of Molecular Psychiatry, Advisor- Eric J. Nestler, M.D., Ph.D., University of Texas Southwestern Medical Center
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2008-2011 Postdoctoral Fellow, Laboratory of Molecular Psychiatry, Advisor- Eric J. Nestler, M.D., Ph.D., Icahn School of Medicine at Mount Sinai
Biosketch
I obtained my PhD in Neuroscience from the University of California, Los Angeles under the mentorship of Dr. X. William Yang. My PhD work involved pioneering a new methodology to isolate the two main striatal projection neuron subtypes for gene expression profiling and uncovered many new genes enriched in these two neuron subtypes. My Postdoctoral studies were performed in the laboratory of Dr. Eric Nestler at University of Texas Southwestern in Dallas, TX and Icahn School of Medicine at Mount Sinai in New York, NY. I built upon my scientific foundations in striatal circuit genetics and function to continue studying these circuits in drug addiction. My work demonstrated divergent roles in the two ventral striatal (nucleus accumbens- NAc) projection neurons in the rewarding effects of cocaine and morphine. I joined the Department of Anatomy and Neurobiology at the University School of Medicine in 2011. My lab continues to study the two striatal projection neuron subtypes, as well as their downstream brain target regions, in psychiatric disease including drug abuse, mood disorders and stereotypy disorders.
Research/Clinical Keywords
Addiction, depression, stereotypy, striatum, nucleus accumbens, neuron subtypes, circuits, gene expression, transcription, epigenetics
Highlighted Publications
- Chandra R, Engeln M, Schiefer C, Patton MH, Martin JA, Werner CT, McGlincy M, Riggs L, Francis TC, Das S, Girven K, Konkolmatt P, Gancarz A, Golden SA, Iniguez S, Russo S, Turecki G, Mathur BN, Creed M, Dietz DM, Lobo MK. (2017) Mitochondrial fission in D1 neuron subtypes mediates cellular and behavioral plasticity during early cocaine abstinence. Neuron. 96:1327-41
- Francis TC, Chandra R, Gaynor A, Konkalmatt P, Ransom Metzbower S, Blanpied TA, Lobo MK. (2017) Molecular basis of dendritic atrophy and activity in stress susceptibility. Molecular Psychiatry. 22:1512-19
- Chandra R, Francis TC, Nam H, Riggs L, Engeln M, Rudzinskas S, Konkalmatt P, Russo S, Turecki G, Iniguez S, Lobo MK. (2017) Reduced Slc6a15 in nucleus accumbens D2-neurons underlies stress susceptibility. The Journal of Neuroscience. 37:6527-38.
- Chandra R, Engeln M, Amgalan A, Fennel M, Chow A, Francis TC, Lobo MK. (2017) A Role for Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1α in Nucleus Accumbens Neuron Subtypes in Cocaine Action. Biological Psychiatry. 81:564-72.
- Francis TC and Lobo MK. (2017) Emerging role for nucleus accumbens medium spiny neuron subtypes in depression. Biological Psychiatry. 81:645-53
- Chandra R, Francis TC, Konkalmatt P, Amgalan A, Gancarz AM, Dietz DM, Lobo MK. (2015) Opposing role for Egr3 in nucleus accumbens cell subtypes in cocaine action. J Neurosci. 35:7927-37
- Francis TC, Chandra R, Friend DM, Finkel E, Dayrit G, Miranda J, Brooks JM, Iñiguez S, O’Donnell P, Kravitz A, Lobo MK. (2015) Nucleus accumbens medium spiny neuron subtypes mediate depression-related outcomes to social defeat stress. Biological Psychiatry. 77:212-22
Additional Publication Citations
- Damez-Werno D, Sun HS, Scobie K, Shao N, Rabkin J, Dias C, Calipari E, Maze I, Pena C, Walker D, Cahill M, Chandra R, Gancarz A, Mouzon R, Landry J, Cates H, Lobo MK, Dietz D, Allis CD, Guccione E, Turecki G, Defilippi P, Never RL, Hurd Y, Shen L, Nestler EJ. Histone Arginine Methylation in Cocaine Action: Essential Role of Src Signaling in the Nucleus Accumbens. PNAS. 113: 9623-8
- Creed MC, Ntamati NR, Chandra R, Lobo MK, Luscher C. (2016) Convergence of reinforcing and anhedonic effects in the ventral pallidum. Neuron. 92: 214-226
- Wright KN, Hollis F, Duclot F, Dossat AM, Strong CE, Francis TC, Mercer R, Feng J, Dietz DM, Lobo MK, Nestler EJ, Kabbaj M. (2015) Methyl Supplementation Attenuates Cocaine-Seeking Behaviors and Cocaine-Induced c-Fos Activation in a DNA Methylation-Dependent Manner. J Neurosci. 35:8948-58
- Kupchick YM, Brown RM, Heinsbroek JA, Lobo MK, Schwartz DJ, Kalivas PW. (2015) Coding the direct/indirect pathways by D1 and D2 receptors is not valid for accumbens projections. Nature Neurosci. 18:930-2
- Francis TC and Lobo MK. (2015) Optogenetic Regulation of Dopamine Receptor-Expressing Neurons. Neuromethods. 96:329-42.
- Francis TC, Chaudhury D, Lobo MK. (2014) Optogenetics: Illuminating the neural basis of rodent behavior. Open Access Animal Physiology. 6:33-51
- Papachatzaki MM, Gaspari S, Koo JW, Tsimpanouli ME, Stergiou E, Bagot RC, Ferguson D, MouzonE, ChacravartyS, DeisserothK, Lobo MK, Zachariou V. (2014) Nucleus Accumbens specific interventions in RGS9-2 activity modulate responses to morphine. Neuropsychopharm. 39: 1968-1977
- Koo JW, Lobo MK, Chaudhury D, Labonte B, Friedman A, Heller W, Pena C, Han M-H, Nestler EJ. (2014) Loss of BDNF signaling in D1R expressing NAc neurons enhances morphine reward by reducing GABA inhibition. Neuropsychopharm. 39:2646-53
- Volman S, Lammel S, Margolis EB, Kim Y, Richard JM, Roitman MF, Lobo MK. (2013) New Insights into the Specificity and Plasticity of Reward and Aversion Encoding in the Mesolimbic System. J Neurosci. 33:17569-76
- Lobo MK, Zaman S, Damez-Werno D, Koo JW, Bagot R, DiNieri J, Nugent A, Finkel E, Chaudhury D, Chandra R, Riberio E, Rabkin J, Mouzon E, Cachope R, Cheer J, Han M-H, Dietz DM, Self D, Hurd Y, Vialou V, Nestler EJ (2013). DFosB Induction in Striatal Medium Spiny Neuron Subtypes in Response to Chronic Pharmacological, Emotional, and Optogenetic Stimuli. J Neurosci. 33:18381-95
- Thanos PK, Robinson L, Nestler EJ, Kim R, Michaelides M, Lobo MK, Volkow ND. Mapping brain metabolic connectivity in awake rats with MicroPET and Optogenetic Stimulation. (2013) J Neurosci. 33:6343-9
- Smith RJ, Lobo MK, Spencer S, Kalivas P. (2013) Cocaine-induced adaptations in D1 and D2 accumbens projection neurons (a dichotomy not necessarily synonymous with direct and indirect pathways). Curr Opin Neurobiol. 23:546-553
- Chandra R, Lenz JD, Gancarz AM, Chaudhury D, Schroeder GL, Han, M-H, Cheer JF, Dietz DM, Lobo MK. Optogenetic inhibition of D1R containing nucleus accumbens neurons alters cocaine- mediated regulation of Tiam1. Frontiers in Molecular Neurosci. 6:13
- Chaudhury D, Walsh JJ, Friedman AK, Juarez B, Ku SM, Koo JW, Ferguson D, Tsai H-C, Pomeranz L, Christoffel DJ, Nectow AJ, Mouzon E, Ekstrand M, Domingos A, Mazei-Robison M, Lobo MK, Neve RL, Friedman JM, Russo SJ, Deisseroth K, Nestler EJ, Han M-H. (2013) Phasic firing of ventral tegmental area dopamine neurons promotes rapid induction of depression behaviours. Nature. 493:532-6
- Lenz JL and Lobo MK. (2013) Optogenetic insights into striatal function and behavior. Behavioural Brain Research. 255:44-54
- Koo JW, Mazei-Robison MS, Chaudhury D, Juarez B, LaPlant Q, Ferguson D, Feng J, Sun H, Scobie KN, Damez-Werno D, Crumiller M, Ohnishi YN, Ohnishi YH, Mouzon E, Dietz DM, Lobo MK, Neve RL, Russo SJ, Han M-H, and Nestler EJ. (2012) BDNF is a Negative Modulator of Morphine Action. Science. 338:124-8
- Dietz DM, Sun H, Lobo MK, Gao V, Chadwick B, Koo JW, Mazei-Robison MS, Damez-Werno D, Maze I, Dietz KC, Ferguson D, Christoffel D, Ohnishi Y, Hodes G, Zheng Y, Neve RL, Russo SJ and Nestler EJ. (2012) Essential role for Rac1 in cocaine-induced structural plasticity of nucleus accumbens neurons. Nature Neurosci. 15:891-6
- Lobo MK, Covington III HE, Chaudhury D, Friedman A.K., Sun H, Damez-Werno D, Dietz DM, Zaman S, Koo JW, Kennedy PJ, Mouzon E, Mogri M, Neve RL, Deisseroth K, Han M-H, Nestler EJ. (2010) Cell-type specific loss of BDNF signaling mimics optogenetic control of cocaine reward. Science. 330: 385-390
Research Interests
For details on our lab's research please visit: www. lobolab.org
Awards and Affiliations
2006 UCLA Dr. Eva Mary Kavan Prize for excellence in research on the brain
2010 2010 NARSAD Young Investigator Award
2011 2011 Blavatnik Award for Young Scientist Finalist Honoree
2011 American College of Neuropsychopharmacology Travel Awardee
2014-present Associate Editor, The Journal of Neuroscience
2016 IMHRO/ Janssen Rising Star Translational Research Awardee
2016 American College of Neuropsychopharmacology (ACNP) Associate Member
2016-present Editorial Advisory Board, ACS Chemical Neuroscience
2017-present Editorial Board, Biological Psychiatry
2017 Presidential Early Career Award for Scientists and Engineers (PECASE) awarded by the Obama administration
Grants and Contracts
NIH/NIDA R01 DA 038613 (Role: PI)- “Cell subtype transcriptional mechanisms in cocaine addiction”
NIH/NIMH R01 MH 106500 (Role: PI)- “The neurocircuitry of depression: Molecular and cell subtype mechanisms”
US-Israel Binational Science Foundation (Role: Co-PI)- “Deciphering the genetics and physiology of the ventral pallidum”
National Science Foundation (Role: Co-PI)- “Focused Electrical Stimulator for Targeted Neuromodulation”
IMHRO /Janssen Rising Star Translational Research Award (Role: PI)- “Stabilizing neuronal architecture for depression therapeutics”
NIH/NIDA R21 DA DA 046227 (Role PI)- “Light activated CRISPR epigenome editing in cocaine abuse”
Lab Techniques and Equipment
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Neuronal subtype gene expression using RiboTag
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Chromatin immunoprecipitation
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Neuronal subtype mitochondrial imaging and analysis
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Neuronal dendritic morphology analysis
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Neuronal subtype genetic targeting using adeno-associated viruses or herpes simplex virus
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In vivo calcium imaging unsing miniaturized fluorescent microscopes
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Optogenetics and chemogenetics
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Animal behavior- drug self administration, conditioned place preference, social defeat stress, anxiety and mood disorder paradigms, stereotypy behavior