James B. Kaper, PhD

Schuller, S., R. Heuschkel, F. Torrente, J.B. Kaper, and A.D. Phillips. Shiga toxin binding in normal and inflamed human intestinal mucosa. Microbes and Infection, 2007, 9:35-39.

Zhu, C., S. Feng, Z. Yang, K. Davis, H. Rios, J.B. Kaper, and E.C. Boedeker. LEE-encoded regulator (Ler) mutants elicit serotype-specific protection, but not cross-protection, against attaching and effacing Escherichia coli strains. Vaccine, 2007, 25:1884-1892.

Rendón, M.A., Z. Saldana, V. Monteiro-Neto, A.L. Erdem, D.R. Hernández, A. Vázquez, J.B. Kaper, J.L. Puente, and J.A. Girón. Commensal and pathogenic Escherichia coli use a common pilus adherence factor for epithelial cell colonization. Proceedings of the National Academy of Sciences, USA, 2007, 104:10637-10642.

Xicohténcatl-Cortes, J., V. Monteiro-Neto, M.A. Rendón, M.A. Ledesma, O. Francetic, P. Samadder, P. Vázquez, J.B. Kaper, J.L. Puente, J.A. Girón. Intestinal Adherance associated with type IV pili of Enterohemorrhagic Escherichia coli O157:H7. Journal of Clinical Investigation, 2007 Nov 1; 117 (11): 3519-3529.

Lombardo, M-J, J. Michalski, H. Martinez-Wilson, N. Maroncle, C.G. Osorio, J.P. Nataro, C.O. Tacket, A. Camilli, and J.B. Kaper. A V. cholerae IVET (In Vivo Expression Technology) screen in human volunteers. Proceedings of the National Academy of Sciences, USA PNAS, 2007, 104:18229-18234

Zhu, C., S. Feng, V. Sperandio, Z. Yang, T.E. Thate, J.B. Kaper, E.C. Boedeker. The possible influence of LuxS in the in vivo virulence of rabbit enteropathogenic Escherichia coli. Veterinary Microbiology, 2007, 125: 313-322.

Gomez-Duarte O.G., Chattopadyay S., Weissman S.J., Giron J.A., Kaper, J.B., Sokuremko E.V., Genetic Diversity of the gene cluster encoding Longus, a type IV pilus of enterotoxigenic Escherichia coli. Journal of Bacteriology, 2007, 189:9145-9149

Bai L., Schuller S., Whale, Mousiner A., Marches O., Wang L., Ooka T., Heushkel R. Torrente F., Kaper J.B., Gomes T.A.,Xu J., Phillips A.D., Frankel G., Enteropathogenic Escherichia coli O125:H6 triggers attaching and effacing lesions on human biopsy specimens independently of Nck and TccP/TccP2. Infection and Immunity, 2008, 76:361-368

Kaper, J.B. and M.A. Karmali. The continuing evolution of a bacterial pathogen. Proceedings of the National Academy of Sciences, USA, 2008 105:4543-4536

Harrison, L.M., P. Rallabhandi, J. Michalski, X. Zhou, S.R. Steyert, S. N. Vogel and J. B. Kaper. Vibrio cholerae flagellins induce Toll-like receptor 5-mediated interleukin-production through mitogen-activated protein kinase and NF-kappaB activation. Infection and Immunity, 2008, 76:5524-5534. PMC 2583592

Lodato, P. B. and J. B. Kaper. Post-transcriptional processing of the LEE4 operon in enterohaemorrhagic Escherichia coli. Molecular Microbiology, 2009, 71:273-290.PMC 2782684

Schüller, S., M. Lucas, J. B. Kaper, J. A. Girón and A. D. The ex vivo response of human intestinal mucosa to enteropathogenic Escherichia coli infection. Cellular Microbiology, 2009, 11:521-30. PMC 2676445

Saldaña, Z, J. Xicohtencatl-Cortes, F. Avelino, A. D. Phillips, J. B. Kaper, J. L. Puente, and J. A. Girón. Synergistic role of curli and cellulose in cell adherence and biofilm frmation of attaching and effacing Escherichia coli and identification of Fis as a negative regulator of curli. Environmental Microbiology, 2009, 11:992-1006. PMC 2672964

Samadder, P., J. Xicohtencatl-Cortes, Z. Saldaña Z, D. Jordan, P. I. Tarr, J. B. Kaper, and J. A. Girón. The Escherichia coli ycbQRST operon encodes fimbriae withlaminin-binding and epithelial cell adherence propterties in Shiga-toxigenic Escherichia coli O157:H7. Environmental Microbiology, 2009, 11:1815-1826. PMC 2888687

Saldaña, Z., A. L. Erdem, S. Schüller, I. N. Okeke, M. Lucas, A. Sivananthan, A. D. Phillips, J. B. Kaper, J. L. Puente, and J. A. Girón. The Escherichia coli common pilus and the bundle-forming pilus act in concert during the formation of localized adherence by enteropathogenic E. coli. Journal of Bacteriology, 2009, 191:3451-3461. PMC 2681888

Hansen, A. M., and J. B. Kaper. Hfq affects the expression of the LEE pathogenicity sland in enterohaemorrhagic Escherichia coli. Molecular Microbiology, 2009, 73:446- 465. PMC 2770234

Morin, C. E. and J. B. Kaper. Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters in the Vibrio cholerae vaccine strain CVD 103-HgR, FEMS Immunology & Medical Microbiology, 2009, 57:69-79. PMC 2906245

Keller, R., T.D. Hilton, H. Rios, E.C. Boedeker, and J.B. Kaper. Development of a live oral attaching and effacing Escherichia coli vaccine candidate using Vibrio cholerae CVD103-HgR as antigen vector. Microbial Pathogenesis, 2010, 48:1-8. PMC 2814881

Caburlotto, G., M. M. Lleò, T. Hilton, A. Huq, R. R. Colwell and J. B. Kaper. Effect on human cells of environmental Vibrio parahaemolyticus strains carrying type III scretion system 2. Infection and Immunity, 2010, 78:3280-7. PMC 897368

Newton, H.J, J. S. Pearson, L. Badea, M. Kelly, M. Lucas, G. Holloway, K. M. Wagstaff, M. A. Dunstone, J. Sloan, J. C. Whisstock, J. B. Kaper, R. M. Robins-Browne, D. A. Jans, G. Frankel, A. D. Phillips, B. S. Coulson, and E. L. Hartland. The type III effectors NleE and NleB from enteropathogenic E. coli and OspZ from Shigella block nuclear translocation of NF-κB p65. PLoS Pathogens, 2010, 13;6:e1000898. PMC 2869321

Habdas, B. J., J. Smart, J. B. Kaper, V. Sperandio. The LysR-type transcriptional regulator QseD alters type three secretion in enterohemorrhagic Escherichia coli and motility in K-12 Escherichia coli. Journal of Bacteriology, 2010, 192:3699-712. PMC 2897335

Steyert, S. R., D. A. Rasko and J. B. Kaper. Functional and phylogenetic analysis of ureD in Shiga toxin-producing Escherichia coli. Journal of Bacteriology, 2011, 193:875-886. PMC3028682

Rhee, K. J., H. Cheng, A. Harris, C. Morin, J. B. Kaper, and G. Hecht. Determination of spatial and temporal colonization of enteropathogenic E. coli and enterohemorhagic E. coli in mice using bioluminescent in vivo imaging. Gut Microbes, 2011, 2;34-41

Torres. A. G., R.J. Cieza, M. Rojas-Lopez, C.A. Blumentritt, C.S. Souza, R.K. Johnston, N. Strockbine, J. B. Kaper, E. Sbrana and V. Popov. In vivo bioluminescence imaging of E. coli O104:H4 and characterization of virulence propertiesin a mouse model of infection. BMC Microbiology, 2012, 12:112.

Steyert, S.R. and J. B. Kaper. Contribution of Urease to the Pathogenicity of Shiga Toxin-Producing Escherichia coli.  Infection and Immunity, 2012, 80:2589-600.

Lodato, P.B., P-K. Hsieh, J. G. Belasco, and J. B. Kaper. A mini-ORF in the leader region protects a T3SS mRNA from degradation by RNase E. Molecular Microbiology, 2012, 86:1167-82.

Faherty, C., J. M. Harper, T. Shea-Donohue, E. M. Barry, J. B. Kaper, A. Fasano, and J. P. Nataro.  Chromosomal and plasmid-encoded factors of Shigella flexneri induce secretogenic activity ex vivo. PLoS One, 2012, 7:e49980.

Hansen, A-M.,  Chaerkady, R., J. Sharma, J. J. Diazmejia, N. Tyagi, S. Renuse, H. K.C. Jacob, S. M. Pinto, N. A. Sahasrabuddhe, M-S. Kim, B. Delanghe, N. Srinivasan, A. Emili, J. B. Kaper, and A. Pande.  The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence. PLoS Pathogens, 2013, in press.

Research in my laboratory focuses on the molecular pathogenesis of enteric bacterial pathogens. Our studies range from basic research on pathogenesis and prokaryotic gene regulation to translational research such as the development of new vaccines and diagnostic probes. The specific pathogens being studied are Vibrio cholerae and diarrheagenic Escherichia coli. Our work with V. cholerae has resulted in the development of an attenuated live oral cholera vaccine that was the first recombinant bacterial vaccine to be tested in and licensed for humans.

We are currently investigating the interaction of V. cholerae and the innate immune system, specifically the activation of TLR5 by V. cholerae flagella, and the in vivo induction of gene expression during infection. Our work with pathogenic E. coli focuses on enteropathogenic E. coli (EPEC), a cause of infant diarrhea, and enterohemorrhagic E. coli O157:H7 (EHEC), a cause of bloody diarrhea and hemolytic uremic syndrome. The latter pathogen has achieved particular notoriety as the cause of large outbreaks of disease due to ingestion of contaminated spinach and hamburgers.

Our research has yielded many insights into the pathogenesis and evolution of these pathogens, including the discovery of a 35,000 bp “pathogenicity island” that mediates epithelial cell adherence and intestinal histopathology, global regulators of virulence gene transcription, a cell-to-cell signaling system (quorum sensing) that regulates virulence gene expression, and diagnostic DNA probes.

• Elected Fellow, American Academy of Microbiology, 1994
• Elected Fellow, American Association for the Advancement of Science, 1994
• Associate Editor, International Journal of Medical Microbiology, 2000 – present
• Merit Award, National Institutes of Health, 2004
• Editor-in-Chief, EcoSal: Escherichia coli and Salmonella Cellular & Molecular Biology, 2006 – present
• Associate Editor, Gut Microbes, 2009 – present
• Elected to Pass & Susel Academy of Educational Excellence, University of Maryland School of Medicine, 2010
• ASM DC White Award for Interdisciplinary Research & Mentoring in Microbiology, American Society for Microbiology, 2019
• James and Carolyn Frenkil Distinguished Dean's Professorship, 2019

• Graduate teaching and Research Assistant, Department of Microbiology, University of Maryland, College Park, Maryland, 1975 – 1979
• Senior Fellow, Department of Microbiology, University of Washington, Seattle, Washington, 1979 – 1981
• Assistant Professor of Microbiology and of Medicine, University of Maryland, Baltimore, 1981 – 1984
• Chief, Bacterial Genetics Section, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, 1981 – present
• Associate Professor of Medicine, Microbiology and Biological Chemistry, University of Maryland School of Medicine, Baltimore, Maryland, 1984 – 1990
• Professor of Medicine (primary appointment), Microbiology and Immunology, Biological Chemistry, University of Maryland School of Medicine, Baltimore, 1990 – 1998
• Professor of Microbiology and Immunology (primary appt.), Biological Chemistry, and Medicine, University of Maryland School of Medicine, Baltimore, 1998 – present
• Chair, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, 2007 – Present
• Senior Associate Dean for Academic Affairs, University of Maryland School of Medicine, Baltimore, 2014 – 2019
• Vice Dean for Academic Affairs, University of Maryland School of Medicine, Baltimore, 2019 - Present

James B. Kaper and Myron M. Levine
"Method of Isolating Restriction Fragment Deletions in Vibrio cholerae, and Products Thereof"
U.S. Patents No. 4,935,364; 5,135,862; and 5,470,729

James B. Kaper and Myron M. Levine
"Vibrio cholerae strain CVD103HgR, Method of Making Same, and Vaccines Derived Therefrom"
U.S. Patents No. 5,399,494 and 5,628,994

James B. Kaper and Myron M. Levine
"Vibrio cholerae O1 (CVD111) and non‑O1 (CVD112 and CVD112RM) Serogroup Vaccine Strains, Methods of Making Same and Products Thereof"
U.S. Patent No. 5,882,653

James B. Kaper and Karen Jarvis
“Immunodiagnostic Test for Enterohemorrhagic Escherichia coli Infection”
U.S. Patent No. 6,204,004

James B. Kaper and Karen Jarvis
“Escherichia coli secreted protein B”
U.S. Patent No. 6,635,259

Multiple international patents for cholera vaccines

Licensed Vaccines:

CVD 103-HgR, a recombinant live oral cholera vaccine
Licensed by the US FDA on June 10, 2016
Previously licensed in numerous countries including Argentina, Australia, Canada, Colombia, Guatemala, el Salvador, Finland, Honduras, Nicaragua, Norway, Peru, the Philippines, Sri Lanka, Sweden, Switzerland, Venezuela.