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Tracy H. Hazen, PhD

Academic Title:

Assistant Professor

Primary Appointment:

Microbiology and Immunology

Location:

Health Sciences Facility III, 670 West Baltimore St, Baltimore 21201

Phone (Primary):

410-706-1954

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Education and Training

I received a Ph.D. in Applied Biology specializing in Microbiology from the Georgia Institute of Technology. During my Ph.D. I studied microbial ecology and the contribution of plasmids and phages to the genetic diversity of bacteria in coastal marine environments. I also used molecular methods to compare the genetic diversity of Vibrio parahaemolyticus isolated from clinical and environmental specimens. I completed my postdoctoral training in the laboratory of Dr. David Rasko at the Institute for Genome Sciences and then joined the faculty of the Department of Microbiology and Immunology and the Institute for Genome Sciences.

Biosketch

Since joining IGS I have used comparative genomics and transcriptomics to investigate the diversity of enteric and nosocomial pathogens. While I have studied numerous diverse microorganisms, the majority of my work has focused on E. coli associated with childhood diarrhea, or multidrug resistant nosocomial pathogens from patients in intensive care units. I have also carried forward my interest in plasmids from my graduate studies and have described the genetic diversity of virulence plasmids from E. coli as well as antibiotic resistance plasmids from E. coli and additional species including Klebsiella. My current research examines the genetic diversity of antibiotic resistant pathogens and I am interested in translational applications of sequencing-based microbial surveillance in healthcare settings for early outbreak detection and infection control.

Research/Clinical Keywords

E. coli, Klebsiella, Acinetobacter, genomics, evolution, antibiotic resistance, microbial surveillance

Highlighted Publications

    1. Hazen, T.H., J.W. Sahl, M.S. Donnenberg, C.M. Fraser, F. Scheutz, and D.A. Rasko.  2013.  Refining the pathovar paradigm via phylogenomics of the attaching and effacing Escherichia coli.  Proc. Natl. Acad. Sci. USA, 110:12810-12815. (PMID: 23858472)
    2. Subashchandrabose, S., T.H. Hazen, A.R. Brumbaugh, S.D. Himpsl, S.N. Smith, R.D. Ernst, D.A. Rasko, and H. Mobley.  2014.  Human-specific induction of Escherichia coli fitness genes during clinical urinary tract infection.  Proc. Natl. Acad. Sci. USA 111: 18327-32. (PMID: 25489107)
    3. Hazen, T.H., L. Zhao, M.A. Boutin, G. Robinson, A.D. Harris, D.A. Rasko, and J.K. Johnson.  2014.  Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiella species isolated from ICU patients: the utility of whole genome sequencing in healthcare settings.  Antimicrob. Agents Chemother. 58: 4814-4825. (PMID: 24914121)
    4. Hazen, T.H., J.B. Kaper, J.P. Nataro, and D.A. Rasko.  2015.  Comparative genomics provides insight into the diversity of the attaching and effacing Escherichia coli (AEEC) virulence plasmids. Infect. Immun. 83:4103-4117. (PMID: 26238712)
    5. Hazen, T.H., M.S. Donnenberg,S. Panchalingam, M. Antonio, A. Hossain, I. Mandomando, J.B. Ochieng, T. Ramamurthy, B. Tamboura, S. Qureshi, F. Quadri, A. Zaidi, K.L. Kotloff, M.M. Levine, E.M. Barry, J.B. Kaper, D.A. Rasko, and J.P. Nataro.  2016.  Genomic diversity of EPEC associated with clinical presentations of differing severity.  Nat. Microbiol. (PMID: 27571975)
    6. Hazen, T.H.,C. Daugherty, A.C. Shetty, A.A. Mahurkar, O.R. White, J.P. Nataro, J.B. Kaper, and D.A. Rasko. 2017.  Transcriptional variation of diverse enteropathogenic Escherichia coli under virulence-inducing conditions. mSystems, (PMID: 28766584).
    7. Hazen, T.H., Michalski, Q. Luo, A.C. Shetty, S.C. Daugherty, J. Fleckenstein, and D.A. Rasko. 2017.  Comparative genomics and transcriptomics of hybrid Escherichia coli isolates carrying virulence factors of both enteropathogenic and enterotoxigenic E. coli.  Sci. Reports, (PMID: 28615618).
    8. Richter, T., H. Hazen, D. Lam, C.L. Coles, J.C. Seidman, Y. You, E.K. Silbergeld, C.M. Fraser, and D.A. Rasko. 2018. Dynamics of Escherichia coli in human populations in Tanzania. mSphere. (PMID: 30404930)
    9. Hazen, T.H., and D.A. Rasko. 2019. Complete genome of the atypical enteropathogenic Escherichia coli archetype isolate E110019 highlights a role of plasmids in dissemination of the type III secreted effector EspT. Infect. Immun. (PMID: 31358567)
    10. Hazen, T.H., S. Nagaraj, E.M. Barry, J. Bitoun, W.H. Chen, S.M. Tennant, and D.A. Rasko. 2019. Comparative genomics of CFA/I and CS6-producing ST-only enterotoxigenic Escherichia coli
      associated with human diarrhea. mSystems. (PMID: 30944874)

    Additional Publication Citations

    1. Hazen, T.H., D. Wu, J.A. Eisen, and P.A. Sobecky.  2007.  Sequence characterization and comparative analysis of three plasmids isolated from environmental Vibrio spp.  Appl. Env. Microbiol. 73:7703-7710. (PMID: 17921277)
    2. Criminger, J.D., T.H. Hazen, P.A. Sobecky, and C.R. Lovell.  2007.  Nitrogen fixation by Vibrio parahaemolyticus and its implications for a new ecological niche.  Appl. Env. Microbiol.73:5959-5961. (PMID: 17675440)
    3. Hazen, T.H., K.D. Kennedy, S. Chen, S.V. Yi, and P.A. Sobecky.  2009. Inactivation of mismatch repair increases the diversity of Vibrio parahaemolyticus.  Environ. Microbiol. 11:1254-1266. (PMID: 19161434)
    4. Sobecky, P.A., and T.H. Hazen.  2009.  Horizontal gene transfer and mobile genetic elements in marine systems.  Horizontal gene transfer:  genomes in flux.  Humana Press.  Eds. M.B. Gogarten et al. 532:435-453. (PMID: 19271200)
    5. Hazen, T.H., R.J. Martinez, Y. Chen, P.C. Lafon, N.M. Garrett, M.B. Parsons, C.A. Bopp, M.C. Sullards, and P.A. Sobecky.  2010.  Rapid identification of Vibrio parahaemolyticus by whole-cell matrix assisted laser desorption ionization-time of flight mass spectrometry.  Appl. Environ. Microbiol.75:6745-6756. (PMID: 19749061)
    6. Hazen, T.H., L. Pan, J.D. Gu, and P.A. Sobecky.  2010.  Contribution of mobile genetic elements to the ecology and evolution of Vibrios.  FEMS Microbiol. Ecol.Review. 74:485-499. (PMID: 20662928)
    7. Hazen, T.H., G. Robinson, A.D. Harris, D.A. Rasko, J.K. Johnson.  2012.  Genome sequence of Klebsiella oxytoca 11492-1, a nosocomial isolate possessing a FOX-5 AmpC β-lactamase.  J. Bacteriol. 194:3028-3029. (PMID: 22582383)
    8. Hazen, T.H., J.W. Sahl, J. Redman, C. Morris, S. Daugherty, M. Chibucos, N. Sengamalay, C. Fraser-Liggett, H. Steinsland, T. Whittam, B. Whittam, S. Manning, and D.A. Rasko.  2012.  Draft genome sequence of diarrheagenic Escherichia coli collection.  J. Bacteriol.  194:3026-3027. (PMID: 22582382)
    9. Subashchandrabose, S., T.H. Hazen, D.A. Rasko, and H. Mobley.  2013.  Draft genome sequences of five recent human uropathogenic Escherichia coli isolates.  Pathogens Dis. (PMID: 23821517)
    10. Nisa, S., T.H. Hazen, L. Assatourian, J.P. Nougayrède, D.A. Rasko, and M.S. Donnenberg.  2013.  In vitro evolution of an archetypal enteropathogenic Escherichia coli strain. J. Bacteriol. 195:4476-4483. (PMID: 23913321)
    11. Hazen, T.H., J.W. Sahl, C.M. Fraser, M.S. Donnenberg, F. Scheutz, and D.A. Rasko.  2013.  Draft genome sequences of three O157 enteropathogenic Escherichia coli isolates.  Genome Announc.  (PMID: 23868135)
    12. Hazen, T.H., J.W. Sahl, M.S. Donnenberg, C.M. Fraser, F. Scheutz, and D.A. Rasko.  2013.  Refining the pathovar paradigm via phylogenomics of the attaching and effacing Escherichia coli.  Proc. Natl. Acad. Sci. USA, 110:12810-12815. (PMID: 23858472).
    13. Levine, J.A., A.M. Hansen, J.M. Michalski, T.H. Hazen, D.A. Rasko, and J. Kaper.  2014.  H-NST induces LEE expression and the formation of attaching and effacing lesions in enterohemorrhagic Escherichia coli. Plos One, 9(1):e86618.  (PMID: 24466172)
    14. Sanjar, F., T. Hazen, S. Shah, S. Koenig, S. Agrawal, S. Daugherty, L. Sadzewicz, L. Tallon, M. Mammel, P. Feng, R. Soderlund, P. Tarr, C. DebRoy, E. Dudley, T. Cebula, J. Ravel, C. Fraser, D. Rasko, and M. Eppinger.  2014.  Genome sequence of Escherichia coli O157:H7 strain 2886-75 associated with the first reported case of human infection in the United States. Genome Announc. 2: e01120-13.  (PMID: 24407635)
    15. Hazen, T.H., L. Zhao, J.W. Sahl, G. Robinson, A.D. Harris, D.A. Rasko, and J.K. Johnson.  2014. Characterization of Klebsiella sp. 10982, a colonizer of humans that contains novel antibiotic resistance alleles and exhibits genetic similarities to plant and clinical Klebsiella isolates.  Antimicrob. Agents Chemother.  58: 1879-88. (PMID: 24395222)
    16. Hazen, T.H., L. Zhao, M.A. Boutin, G. Robinson, A.D. Harris, D.A. Rasko, and J.K. Johnson.  2014.  Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiellaspecies isolated from ICU patients: the utility of whole genome sequencing in healthcare settings.  Antimicrob. Agents Chemother. 58: 4814-4825. (PMID: 24914121)
    17. Hazen, T.H., M.S. Humphrys, B. Ochieng, M. Parsons, C.A. Bopp, E. Mintz, C. O’Reilly, and D.A. Rasko.  2014.  Draft genome sequences of nine enteropathogenic Escherichia coli from Kenya.  Genome Announc. e00582-14 (PMID: 24926061)
    18. Sadiq, S.M., T.H. Hazen, D.A. Rasko, M. Eppinger.  2014.  EHEC Genomics: Past, present and future.  Microbiol. Spectr.  (PMID: 26104203)
    19. Doi, Y., T.H. Hazen, M. Boitano, Y-C. Tsai, T.A. Clark, J. Korlach, D.A. Rasko.  2014.  Whole genome assembly of a Klebsiella pneumoniae strain co-producing NDM-1 and OXA-232 carbapenemases using single-molecule, real-time sequencing.  Antimicrob. Agents Chemother. 58: 5947-53. (PMID: 25070096)
    20. Subashchandrabose, S., T.H. Hazen, A.R. Brumbaugh, S.D. Himpsl, S.N. Smith, R.D. Ernst, D.A. Rasko, and H. Mobley.  2014.  Human-specific induction of Escherichia coli fitness genes during clinical urinary tract infection.  Proc. Natl. Acad. Sci. USA 111: 18327-32. (PMID: 25489107)
    21. Brumbaugh, A., S. Smith, S. Subashchandrabose, S. Himpsl, T. Hazen, D. Rasko, and H. Mobley.  2015.  Blocking yersiniabactin import attenuates extraintestinal pathogenic Escherichia coli in cystitis and pyelonephritis and represents a novel target to prevent urinary tract infection.  Infect. Immun. 83: 1443-50. (PMID: 25624354)
    22. Hazen, T.H., P.C. Lafon, N.M. Garrett, T.M. Lowe, D.J. Silberger, L.A. Rowe, M. Frace, M.B. Parsons, C.A. Bopp, D.A. Rasko, and P.A. Sobecky.  2015.  Insights into the environmental reservoir of pathogenic Vibrio parahaemolyticus using comparative genomics.  Front. Microbiol. (PMID: 25852665)
    23. Donnenberg, M., T.H. Hazen, T.H. Farag, S. Panchalingam, M. Antonio, A. Hossain, I. Mandomando, J.B. Ochieng, T. Ramamurthy, B. Tamboura, A. Zaidi, M.M. Levine, K. Kotloff, D.A. Rasko, and J.P. Nataro.  2015.  Bacterial factors associated with lethal outcome of enteropathogenic Escherichia coli infection: genomic case-control studies.  Submitted to Plos Negl. Trop. Dis. (PMID: 25978422)
    24. Sanjar, F., B. Rusconi, T. Hazen, S. Eppinger, M. Mammel, P. Feng, D. Rasko, and M. Eppinger.  2015.  Characterization of the pathogenome and phylogenomic classification of enteropathogenic Escherichia coli of the O157:non-H7 serotypes.  Pathog. Dis. (PMID: 25962987)
    25. Hazen, T.H., S.C. Daugherty, A.C. Shetty, A.A. Mahurkar, O. White, J.B. Kaper, and D.A. Rasko.  2015.  RNA-Seq analysis of isolate- and growth phase-specific differences in the global transcriptomes of enteropathogenic Escherichia coli prototype strains.  Front. Microbiol. (PMID: 26124752)
    26. Hazen, T.H., J.B. Kaper, J.P. Nataro, and D.A. Rasko.  2015.  Comparative genomics provides insight into the diversity of the attaching and effacing Escherichia coli (AEEC) virulence plasmids. Infect. Immun. 83:4103-4117. (PMID: 26238712)
    27. Kessler, R., S. Nisa, T.H. Hazen, A. Horneman, A. Amoroso, D.A. Rasko, and M.S. Donnenberg.  2015.  Diarrhea, bacteremia and multiorgan dysfunction due to an extraintestinal pathogenic Escherichia coli strain with divergent enteropathogenic E. coli genes. Pathog. Dis. (PMID: 26410828)
    28. Hazen, T.H., M.S. Donnenberg,S. Panchalingam, M. Antonio, A. Hossain, I. Mandomando, J.B. Ochieng, T. Ramamurthy, B. Tamboura, S. Qureshi, F. Quadri, A. Zaidi, K.L. Kotloff, M.M. Levine, E.M. Barry, J.B. Kaper, D.A. Rasko, and J.P. Nataro.  2016.  Genomic diversity of EPECassociated with clinical presentations of differing severity.  Nat. Microbiol. (PMID: 27571975)
    29. Kania, D.A., T.H. Hazen, A. Hossain, J.P. Nataro, and D.A. Rasko.  2016.  Genome dynamics of Shigella boydii.  Pathog. Dis. (PMID: 27056949)
    30. Hazen, T.H., S.R. Leonard, K.A. Lampel, D.W. Lacher, A.T. Maurelli, and D.A. Rasko.  2016.  Investigating the relatedness of enteroinvasive Escherichia coli to other E. coli and Shigella using comparative genomics.  Infect. Immun. 84:2362-71 (PMID: 27271741)
    31. Chibucos, M.C., S. Soliman, T. Ghebremarimam, H. Lee, S. Daugherty, J. Orvis, A.C. Shetty, J. Crabtree, T.H. Hazen, K.A. Etienne, P. Kumari, T.D. O’Connor, D.A. Rasko, C.M. Fraser, S.R. Lockhart, C.D. Skory, A.S. Ibrahim, and V.M. Bruno.  2016.  An integrated genomic and transcriptomic survey of mucormycosis-causing fungi.  Nat. Commun. (PMID: 27447865)
    32. Hazen, T.H., M. Michalski, I. Okeke, and D.A. Rasko. 2017. Characterization of a large antibiotic resistance plasmid found in enteropathogenic Escherichia coli strain B171 and its relatedness to plasmids of diverse E. coli and Shigella. Antimicrob. Agents Chemother. (PMID: 28674052).
    33. Santiago, A.E., M.B. Yan, H. Hazen, B. Sauder, M. Meza-Segura, David A Rasko, M.M. Kendall, F. Ruiz-Perez, and J.P. Nataro. 2017. The AraC negative regulator family modulates the activity of histone-like proteins in pathogenic bacteria. PLoS Pathogens. (PMID: 28806780)
    34. Watkins, T.N., H. Liu, M. Chung, H. Hazen, J.D. Hotopp, S.G. Filler, and V. Bruno. 2018.  Comparative transcriptomics of Aspergillus fumigatus strains upon exposure to human airway epithelial cells. Microb. Genom. (PMID: 29345613)
    35. Robertson, C.D., H. Hazen, J.B. Kaper, D.A. Rasko, and A.M. Hansen. 2018. Phosphotyrosine-mediated regulation of enterohemorrhagic Escherichia coli virulence. mBio. (PMID: 29487233)
    36. Hazen, T.H., R.T. Mettus, C.L. McElheny, S.L. Bowler, Y. Doi, and D.A. Rasko.  2018. Draft genome sequences of blaKPC-containing Enterobacter aerogenes, Citrobacter freundii, and C. koseri strains. genomeA. (PMID: 29472325)
    37. Hazen, T.H., Mettus, C.L. McElheny, S.L. Bowler, S. Nagaraj, Y. Doi, and D.A. Rasko. 2018. Diversity among blaKPC-containing plasmids in Escherichia coli and other bacterial species isolated from the same patients. Sci. Reports. (PMID: 29980699)
    38. Ndungo, E., A. Randall, T.H. Hazen, D. Kania, K. Trappl-Kimmons, X. Liang, E.M. Barry, K. Kotloff, S. Chakraborty, S. Mani, D.A. Rasko, and M. Pasetti. 2018. A novel Shigella proteome microarray discriminates targets of human antibody reactivity following oral vaccination and experimental challenge. mSphere. (PMID: 30068560)
    39. Higginson, E.E., G. Ramachandran,H. Hazen, D.A. Kania, D.A. Rasko, M.F. Pasetti, M.M. Levine, and S.M. Tennant. 2018. Improving our understanding of Salmonella Paratyphi B through the engineering and testing of a live-attenuated vaccine strain. mSphere. (PMID: 30487152)
    40. Vidal, R.M., K. Muhsen, S.M. Tennant, A.M. Svennerholm, S. Sow, D. Sur, A.K.M. Zaidi, A.S.G. Faruque, D. Saha, R. Adegbola, M.J. Hossain, P.L. Alonso, R.F. Breiman, Q. Bassat, B. Tamboura, D. Sanogo, U. Onwuchekwa, B. Manna, T. Ramamurthy, S. Kanungo, S. Ahmed, S. Qureshi, F. Quadri, A. Hossain, S.K. Das, M. Antonio, Inacio Mandomando, Tacilta Nhampossa, Sozinho Acacio, Richard Omore, J.B. Ochieng, J.O. Oundo, E.D. Mintz, C.E. O'Reilly, L.Y. Berkeley, S. Livio, S. Panchalingam, D. Nasrin, T.H. Farag, Y. Wu, H. Sommerfelt, R.M. Robins-Browne, F. Del Canto, T.H. Hazen, D.A. Rasko, K.L Kotloff, J.P. Nataro, and M.M. Levine. 2018. Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea in the Global Enteric Multicenter Study (GEMS). PLoS Negl. Trop. Dis. (PMID: 30608930)
    41. Bornstein, K., S.M. Tennant, H. Hazen, J. Sorkin, M.D. Tapia, S.O. Sow, U. Onwuchekwa, M.M. Levine, D.A. Rasko. 2019. Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali. PLoS Negl. Trop. Dis. (PMID: 31170153)
    42. Kuhlmann, F.M., J. Martin, H. Hazen, T.J. Vickers, M. Pashos, P.C. Okhuysen, O.G. Gómez-Duarte, E. Cebelinski, D. Boxrud, F. del Canto, R. Vidal, F. Qadri, M. Mitreva, D.A. Rasko, and J.M. Fleckenstein. 2019. Conservation and global distribution of non-canonical antigens in Enterotoxigenic Escherichia coli. PLoS Negl. Trop. Dis. (PMID: 31756188)
    43. Rasko, D.A., F. Del Canto, Q. Luo, J.M. Fleckenstein, R. Vidal, and H. Hazen. 2019.  Comparative genomic analysis and molecular examination of the diversity of enterotoxigenic Escherichia coli isolates from Chile. PLoS Negl. Trop. Dis. (PMID: 31747410)
    44. Adedrian, T., S. Hitchcock, L. O'Hara, J. Michalski, J.K. Johnson, D. Calfee, L. Miller, H. Hazen, D.A. Rasko, and A.D. Harris. 2019. Examination of 388 Staphylococcus aureus isolates from intensive care unit patients. Microb. Res. Announc. (PMID: 31727718)
    45. Hazen, T.H.,K. Johnson, A.D. Harris, and D.A. Rasko. 2020. Genome sequencing of Escherichia coli andKlebsiella pneumoniae isolates that harbor the FOX-5 beta-lactamase gene. Microb. Res. Announc. (PMID:33154016)
    46. Hazen, T.H., H. Poonawala, K. Saharia, M. Donnenberg, and D.A. Rasko. Draft genome sequence of Escherichia coli strain UMD142. Microb. Res. Announc. (PMID: 33154008)
    47. Lee, V., R. Ghodssi, N. El-Sayed, R. Malik, O. Goloubeva, T.H. Hazen, and D.A. Rasko. 2020. Draft genome sequence of Pseudomonas aeruginosa strain PA14-UM. Microb. Res. Announc. (PMID: 33184157)
    48. Hazen, T.H., Hitchcock, L. O'Hara, J. Michalski, J.K. Johnson, D. Calfee, L. Miller, A.D. Harris, and D.A. Rasko. 2020. Draft genome sequences of five diverse Klebsiella species isolates from intensive care unit patients. Microb. Res. Announc. (PMID: 33214307)
    49. Sikorski, M., T.H. Hazen, G. Vyas, J. Michalski, and D.A. Rasko. 2020. Genome sequences of four Shigella boydiistrains representative of the major S. boydii clades. Microb. Res. Announc. (PMID: 33033129)
    50. Adedrian, T., S. Hitchcock, L. O'Hara, J. Michalski, J.K. Johnson, D. Calfee, L. Miller, H. Hazen, A.D. Harris, and D.A. Rasko. 2020. Examination of Staphylococcus aureus isolates from the gloves and gowns of intensive care unit healthcare workers. Microb. Res. Announc. (PMID: 32763936)
    51. Risteen, R., H. Hazen, J. Michalski, F. Poly, S. Tennant, and D.A. Rasko. 2020. Examination of 189 Campylobacter species isolates from the Global Enteric Multicenter Study. Microb. Res. Announc. (PMID:32703838)
    52. Hernandes, R.T., T.H. Hazen, L.F. dos Santos, T.K.S. Richter, J.M. Michalski, D.A. Rasko. 2020. Comparative genomic analysis provides insight into the phylogeny and virulence of atypical enteropathogenic Escherichia coli strains from Brazil. PLoS Negl. Trop. Dis. (PMID: 32479541)
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