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CONTACT:

Director

Rena Lapidus, PhD
rlapidus@som.umaryland.edu

Antonino Passaniti, PhD
TPassaniti@som.umaryland.edu

Translational Laboratory Staff

Katharina Richard, PhD

Brandon Carter-Cooper, MS

Xinrong Ma, MD
Kayla Tighe, BS
Andrea Casildo, BS

LOCATION:

Bressler Research Building
Room 7-010
655 West Baltimore Street
Baltimore, MD 21201

HOURS:

Monday through Friday
7:00am - 5:00pm

PHONE:

Office: 410-706-2171

MISSION

The University of Maryland Greenebaum Comprehensive Cancer Center Translational Laboratory Shared Service (TLSS) offers pre-clinical and clinical experimental support to basic researchers and physicians in the UMGCCC community. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.

New facilities give faculty greater access to sophisticated instrumentation, as well as highly-trained technical staff who can offer support to faculty on experimental design, data analysis and interpretation, and provide training opportunities for graduate and medical students, postdoctoral fellows, and faculty within the University and its collaborators.

SERVICES

In Vitro Assays

• Mycoplasma testing
• Clonogenic Survival Assays
• IC50 generation
• Cell cycle (propidium iodide)
• Viability (trypan blue exclusion)
• Apoptosis
• Potentiation/Synergy
• ROS
• Western Analysis
• Angiogenesis

CRISPR Services:

• Knock Down and Single Nucleotide Polymorphism

 

In Vivo Assays

• IACUC approved umbrella protocol
• Tolerability
• Tumor Growth
• Patient Derived Xenograft Models
• Pharmacokinetics: generation of plasma
• Efficacy (flank models)
• Efficacy (orthotopic models)
• Pharmacodynamic Endpoints
• Imaging of cells with Xenogen System

Pharmacodynamic (PD) Endpoints

• in-patient samples, tumor or surrogate tissues, preclinical samples
• Endpoint dependent on target (e.g., ELISA, flow cytometry, Western, unique assay)

RESOURCES

• Access to >120+ human/murine cell lines
• Luciferase-expressing breast, leukemia, ovarian and prostate cancer cell lines
• IACUC approved umbrella protocol
• Access/Knowledge in Using Xenogen/IVIS Imaging Mice
• Primary Derived Xenograft Models
  • Breast
  • Head and Neck (under development)
  • Leukemia (under development)
  • Ovarian (under development)
  • Pancreatic (under development)
• Access to IRB approved protocol for tissue acquisition

Clinical Trial Support:

We isolate:

• Plasma
• Serum
• Whole Blood (isolation of PBMC, DNA, RNA, protein)
• Bone Marrow (isolation of marrow cells)
• Staining of isolated lymphocyte cells
• Coordination with Flow Cytometry Core for analysis
• Exosomes and ct DNA