CIBR: Center for Innovative Biomedical Resources
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Scanning Electron MicroscopeThe FEI Quanta 200 is a versatile high performance, low-vacuum scanning electron microscope with a tungsten electron source. It can be operated in three different vacuum modes, High Vacuum (HV), Low vacuum (LV) and Environmenal mode (ESEM), thus accommodating a wide range of samples of any SEM system.

High vacuum or conventional mode may be used to examine dry conductive specimens. Low vacuum (0.1-1.0 Torr) mode may be used to examine dry specimens that are not inherently conductive and will not tolerate normal coating procedures. Environmental (1.0 – 20 Torr) or wet mode maintains humidity around the specimen whereby enabling examination of hydrated specimens. The Quanta 200 is also equipped with a Gatan Cryo transfer unit (ALTO2100). All images are acquired digitally.


Features and Specifications

  • Electron Optics Source: Tungsten hairpin filament. Voltage: 200 V to 30 kV Beam Current: <2 µA
    Resolution SE resolution: 3.0 nm at 30 kV in high-vacuum, low-vacuum and ESEM operating modes; 10 nm at 3 kV in high-vacuum mode, <12 nm at 3 kV in low-vacuum mode, BSE resolution: 4.0 nm at 30 kV.
  • Vacuum: High-vacuum mode (typically 10-5 mbar) for imaging of conductive and/or conventionally prepared specimens; Low-vacuum mode (<1.3 mbar) for imaging of non-conductive specimens without preparation; ESEM™ mode (<26 mbar = 2600 Pa = 20 Torr) for high-vacuum incompatible specimens which are impossible to investigate with traditional EM methods.
  • Sample Navigation: motorized x-y-z-rotate stage, providing the following movements: x = y = 50 mm (motorized) z = 25 mm (motorized) Z = 25 mm (manual) Tilt +75 degrees to –15 degrees (manual) Tilt eucentric at analytical working distance of 10 mm.
  • Focus Range: 3 – 99 mm Magnification 6x (at longest working distance) to >1,000,000x Field of View Identical field of view in high- and low-vacuum modes (18 mm at the longest working distance) 500 µm with standard, axial, gaseous secondary electron (SE) detector.
  • Scanning System: The Quanta 200 provides a flexible scanning system controlled from the graphical user interface: - Pixel density 512 x 442, 1024 x 884, 2048 x 1768 or 4096 x 3536, selectable; - Minimum dwell time 50 ns/pixel; maximum 1 ms/pixel. - Electronic scan rotation by n x 360 degrees.
  • Detector Systems: The Quanta 200 is equipped with several secondary electron detectors (SED, LF-GSED and GSED), that are optimized for use in high-vacuum, low-vacuum and ultra-low vacuum (ESEM) chamber environments. STEM detector can be used for imaging of TEM prepared samples.
  • Imaging: Images are displayed in an area of 1024 x 884 pixels, configurable for either single-frame or four-quadrant display. Images can be viewed live, averaged or integrated.

Featured SEM Images

Scanning Electron Microscope

Scanning Electron Microscope

Scanning Electron Microscope

Scanning Electron Microscope

Scanning Electron Microscope

Scanning Electron Microscope