Visit the CRISPR Services Website
CONTACT:
Director
Rena Lapidus, PhD
rlapidus@som.umaryland.edu
rlapidus@som.umaryland.edu
Antonino Passaniti, PhD
TPassaniti@som.umaryland.edu
TPassaniti@som.umaryland.edu
Translational Laboratory Staff
Katharina Richard, PhD
Brandon Carter-Cooper, MS
Xinrong Ma, MD
Kayla Tighe, BS
Andrea Casildo, BS
Kayla Tighe, BS
Andrea Casildo, BS
LOCATION:
Bressler Research Building
Room 7-010
655 West Baltimore Street
Baltimore, MD 21201
HOURS:
Monday through Friday
7:00am - 5:00pm
PHONE:
Office: 410-706-2171
MISSION
The University of Maryland Greenebaum Comprehensive Cancer Center Translational Laboratory Shared Service (TLSS) offers pre-clinical and clinical experimental support to basic researchers and physicians in the UMGCCC community. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.
New facilities give faculty greater access to sophisticated instrumentation, as well as highly-trained technical staff who can offer support to faculty on experimental design, data analysis and interpretation, and provide training opportunities for graduate and medical students, postdoctoral fellows, and faculty within the University and its collaborators.
SERVICES
In Vitro Assays
- Mycoplasma testing
- Clonogenic Survival Assays
- IC50 generation
- Cell cycle (propidium iodide)
- Viability (trypan blue exclusion)
- Apoptosis
- Potentiation/Synergy
- ROS
- Western Analysis
- Angiogenesis
CRISPR Services:
- Knock Down and Single Nucleotide Polymorphism
In Vivo Assays
- IACUC approved umbrella protocol
- Tolerability
- Tumor Growth
- Patient Derived Xenograft Models
- Pharmacokinetics: generation of plasma
- Efficacy (flank models)
- Efficacy (orthotopic models)
- Pharmacodynamic Endpoints
- Imaging of cells with Xenogen System
Pharmacodynamic (PD) Endpoints
- in-patient samples, tumor or surrogate tissues, preclinical samples
- Endpoint dependent on target (e.g., ELISA, flow cytometry, Western, unique assay)
RESOURCES
- Access to >120+ human/murine cell lines
- Luciferase-expressing breast, leukemia, ovarian and prostate cancer cell lines
- IACUC approved umbrella protocol
- Access/Knowledge in Using Xenogen/IVIS Imaging Mice
- Primary Derived Xenograft Models
- Breast
- Head and Neck (under development)
- Leukemia (under development)
- Ovarian (under development)
- Pancreatic (under development)
- Access to IRB approved protocol for tissue acquisition
Clinical Trial Support:
We isolate:
- Plasma
- Serum
- Whole Blood (isolation of PBMC, DNA, RNA, protein)
- Bone Marrow (isolation of marrow cells)
- Staining of isolated lymphocyte cells
- Coordination with Flow Cytometry Core for analysis
- Exosomes and ct DNA