Transgenic and Knockout Core Facility
  • My UMB
  • Directory
  • Campus Map
  • Site Map
  • Outlook Email
  • Facebook
  • Twitter
  • YouTube
Make a Gift UM Shuttle
  • About Us
    • About Us
    • Our Mission Vision & Values
    • Our History
    • Our Rankings
    • About Dean Reece
    • State of the School
    • Dean's Senior Staff
    • Administration & Governance
    • Community Engagement
    • Contact Us
  • Prospective Students
    • Office of Admissions
    • Welcome
    • Meet Our Students
    • Graduate Program in Life Sciences
    • Combined Degree Programs
    • Master's Programs
    • Allied Health Programs
  • Student Life
    • Student Profiles
    • Student Support
    • Our Campus
    • About Baltimore
    • Student Events
    • Student Outreach
    • Student Policies
  • Faculty
    • Faculty Profiles
    • Faculty Resources
    • Faculty Excellence Awards/Memberships
    • Department Chairs
  • Research
    • Office of Research
    • Core Facilities
    • UMB Office of Research & Development
    • Human Research Protections Office
    • CICERO
    • COEUS
    • UMB BioPark
    • General Clinical Research Center (GCRC)
    • Graduate Program in Life Sciences
    • Office of Postdoctoral Scholars
    • Office of Student Research
    • Center for Clinical Trials
    • Program in the Biology of Model Systems
    • Kernan Orthopaedics & Rehabilitation
    • Center for Biomolecular Therapeutics (CBT)
  • Patient Care
    • Patient Care Overview
    • University of Maryland Medical Center (UMMC)
    • R Adams Cowley Shock Trauma Center
    • VA Maryland Medical Systems
    • Faculty Physicians Inc. (FPI)
    • University of Maryland Medical System (UMMS)
    • Kernan Orthopaedics & Rehabilitation
  • Alumni & Giving
    • Alumni Resources
    • Office of Development
    • Medical Alumni Association
  • Departments
    • Anatomy & Neurobiology
    • Anesthesiology
    • Biochemistry & Molecular Biology
    • Dermatology
    • Diagnostic Radiology & Nuclear Medicine
    • Emergency Medicine
    • Epidemiology & Public Health
    • Family & Community Medicine
    • Medical & Research Technology
    • Medicine
    • Microbiology & Immunology
    • Neurology
    • Neurosurgery
    • Obstetrics, Gynecology and Reproductive Sciences
    • Ophthalmology & Visual Sciences
    • Orthopaedics
    • Otorhinolaryngology - Head & Neck Surgery
    • Pathology
    • Pediatrics
    • Pharmacology
    • Physical Therapy & Rehabilitation Science
    • Physiology
    • Psychiatry
    • Radiation Oncology
    • Surgery
  • Programs
    • Comparative Medicine
    • Minority Health & Health Disparities
    • Neuroscience
    • Oncology
    • Personalized & Genomic Medicine
    • Trauma
  • Research Centers
    • Biomedical Engineering and Technology (BioMET)
    • Health Policy
    • Integrative Medicine
    • MD Psychiatric Research Center (MPRC)
    • Research on Aging
    • Stem Cell Biology & Regenerative Medicine
    • Trauma & Anesthesiology
    • Vaccine Development
    • Vascular & Inflammatory Diseases
  • Institutes
    • Institute for Genome Sciences
    • Institute of Human Virology
    • Clinical and Translational Sciences Institute
  • Graduate Programs
    • Graduate Program in Life Sciences
      • Biochemistry
      • Molecular Microbiology & Immunology
      • Molecular Medicine
      • Neuroscience
      • Physical Rehabilitation Science
      • Toxicology
      • Gerontology
      • Epidemiology & Human Genetics
      • PT & Rehabilitation Science (PhD, DPT)
    • Combined Degrees
    • Master’s Programs
  • Offices of the Dean
    • Academic Administration
    • Admissions
    • Development
    • Faculty Affairs & Professional Development
    • Human Research Protections
    • Information Services
    • Medical Education
    • Policy & Planning
    • Postdoctoral Scholars
    • Public Affairs
    • Research
    • Student Affairs
    • Student Research
  • Transgenic Core Home
  • Success Rate Data
  • Services & Costs
  • Protocols
  • Forms
    • UMB Investigators
    • Non-UMB Investigators
  • Outside Sources
  • History
  • Prior Approvals
    • Office of Animal Welfare Assurance
    • IBC/Research Registration/Recombinant DNA
  • Staff/Contact Us
Bookmark and Share
Home > Core Facilities > Transgenic : Protocols

Protocols

Microinjection

  • BAC DNA Preparation for Microinjection 

  • Purification of DNA for Pronuclear Injection  

Transfection

  • Purification of DNA for ES Cell Transfection  

  • Isolation of DNA for Mini-Southern Blots From 96-Well Plates
    • Karen Janowski's 
    • A.L. Joyner's 
     

  • Isolation of gDNA from ES Cell Cultures  

Genotyping

  • Calculation of Copy Number Standards for PCR Screening Assays 

  • Harvest Tail Biopsies for gDNA Isolation  

  • Isolation of gDNA from Tail Biopsies 

  • Webmaster
  • Contact Us
  • Outlook Email
  • Logos
  • Rss Feeds
  • Site Index
  • Calendar
  • University of Maryland |
  • University of Maryland Medical Center
© University of Maryland School of Medicine, 655 W. Baltimore Street, Baltimore MD 21201
  • University of Maryland Baltimore
  • University of Maryland Medical Center
Hotline