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Success Rate Data
Injection History of UM-SOM Transgenic Core Facility
(opened January 2004)
| Founder Production From Pronuclear Injection Projects | |||
| Dr. Chandrasekaran | Anesthesiology | pTRE2-IFN Gamma | 8 founders |
| Dr. Sussman | Pharmaceutical Sciences | pLEF-EGFP | 5 founders |
| (All the following constructs were injected into the C57Bl6 strain of mice.) | |||
| Dr. Gong | Medicine | G6665 G6719 G6702 |
2 founders 2 founders 6 founders |
| Dr. Chandrasekaran | Anesthesiology | pTRE2-Mito-EYFP | 6 founders |
| Dr. Gudkov | Cleveland Clinic | pLV-WAF-ConA-luc | 4 founders |
| (This was a lentiviral construct.) | |||
| Chimera Production from Blastocyst Injection | |||
| Dr. Litwack | Anatomy/Neurobiology | MDGA1 ko | 7 chimeras |
| (These chimeras have transmitted thru the germline.) | |||
| Dr. Margolis | Anatomy/Neurobiology | JHKFLM-01 ko | 6 chimeras |
| (These chimeras have transmitted thru the germline, and are now homozygous/hemizygous.) | |||
| Dr. Hamburger | Cancer Center | Ebp1 ko | 0/99 pups |
Previous Injection History of Director
Lerner Research Institute, Cleveland Clinic Foundation
| Founder Production from Pronuclear Injection Projects | |
| At least 1 founder produced | 43/45 projects, 95% successful |
| At least 2 founders produced | 41/45 projects, 91% successful |
| Yearly Statistics | |
| 1999 | 47 founders for 11 projects, average 4.3, range 2-11 |
| 2000 | 64 founders for 18 projects, average 3.5, range 1-11 |
| 2001 | 90 founders for 14 projects, average 6.4, range 4-12 |
| Chimera Production from Blastocyst Injection | |
| At least 1 chimera produced | 9/10 projects, 90% successful |
| At least 5 chimeras produced | 7/10 projects, 70% successful |
| Yearly Statistics | |
| 1999 | 18 chimeras/1 project |
| 2000 | 60 chimeras/6 projects |
| 2001 | 18 chimeras/3 projects |