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Cryopreservation Request Form

Principal Investigator

Name:

Ext:

E-mail:

Is PI a Greenebaum Cancer Center member?
(If so, subsidies may apply; please consult facility director.)

Laboratory Contact

Name:

Ext:

E-mail:

Department:

UM Greenebaum Cancer Center member (subsidies may apply)

FAS Account Number:

Background Strain of Mouse to be Frozen:

Name and Type of Mouse (Transgenic, Knockout, etc.) to be Frozen:

Are mice Homozygous or Heterozygous?

Room and Building Where Mice are Housed:

The facility will require at least 5 adult male mice (2-8 months of age) and preferably these males will be proven fertile or experienced breeders. These mice will be mated to purchased superovulated female mice of the same or a closely related strain. A test freeze, followed by an immediate thaw, will be performed to determine how many embryos need to be frozen to guarantee recovery of the line. The laboratory will be required to genotype these frozen/thawed pups to confirm recovery of the genotype. The males can be returned after the cryopreservation is complete.

Do you anticipate requesting thaws of these mice in the next six months?

An initial consultation with the facility director is required.