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Joseph R Lakowicz Ph.D.

Academic Title: Professor
Primary Appointment: Biochemistry and Molecular Biology
jlakowicz@som.umaryland.edu
Location: UMBI
Phone: (410) 706-8409

Personal History:

Education

  • 1973: Ph.D. in Biochemistry, University of Illinois, Urbana, Illinois
  • 1971: M.S. in Biochemistry, University of Illinois, Urbana, Illinois
  • 1970: B.A. in Chemistry, Maxima Cum Laude, LaSalle College, Philadelphia, Pennsylvania
  • 1969: Temple University, Philadelphia, Pennsylvania (Additional mathematics courses, Summer)
  • 1968: Drexel Institute of Technology, Philadelphia, Pennsylvania (Additional mathematics courses, Summer)

Professional Experience

  • 1984-present: Professor, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, School of Medicine
  • 1993-present: Adjunct Professor, Electrical Engineering, University of Maryland, Baltimore County
  • 1988-present: Senior Staff Scientist, Medical Biotechnology Center, University of Maryland at Baltimore
  • 1988-present: Director, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology
  • 1989-1993: Head, Molecular Graphics Facility, University of Maryland School of Medicine
  • 1980-1984: Associate Professor, Department of Biological Chemistry, University of Maryland at Baltimore, School of Medicine
  • 1975-1980: Assistant Professor of Biochemistry, Department of Biochemistry and Gray Freshwater Biological Institute, University of Minnesota. Promoted to Associate Professor with tenure, effective July 1980
  • 1974-1975: Research Associate, Freshwater Biological Institute, University of Minnesota
  • 1973-1974: Postdoctoral Fellow, Oxford University, Department of Biochemistry, Oxford, England, Nuclear Magnetic Resonance

Research Interests:

This laboratory is involved in the use and development of fluorescence spectroscopic methods. Our present research projects to advance fluorescence technology include two-photon induced fluorescence and the use of light to modify the excited state populations.

The continued development of advanced fluorescence methods is performed at the Center for Fluorescence Spec-tro-sco-py, which is a National Resource of the NIH. This cen--ter provides state-of-the-art time and frequency-domain measurements to users from this campus and from other institutions in this country as well as other countries. Our basic science projects include the use of fluorescence to study:

  • Conformational distributions of proteins, nucleic acids, and transfer RNA.
  • Dynamics of proteins and membranes.
  • Transient effects in diffusion in solutions, and in proteins and membranes.
  • Synthesis of fluorescent sensor molecules.
  • Development of fluorescent lifetime imaging microscopy. Multi-photon excitation.

Publications:

A Wavelength-Ratiometric Fluoride-Sensitive Probe Based on the Quinolinium Nucleus and Boronic Acid Moiety, Badugu, R., Lakowicz, J. R., and Geddes, C. D. (2005). Sensors Actuators B 104:103-110.

Fluorescence Enhancement of Fluorophores Tethered to Different Sized Silver colloids Deposited on Glass Substrate, Lukomska, J., Malicka, J., Gryczynski, I., Leonenko, Z., and Lakowicz, J. R. (2005). Biopolymers 77:31-37.

Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films, Lukomska, J., Gryczynski, I., Malicka, J., Makowiecz, S., Lakowicz, J., and Gryczinski, Z. (2005). Biochemical and Biophysical Research Communications, 328: 78-84.

Nanogold Plasmon Resonance-Based Glucose Sensing. 2. Wavelength- Ratiometirc Resonance Light Scattering, Aslan, K., Lakowicz, J., and Geddes C.D. (2005). Analytical Chemistry, 77: 2007-2014.

Metal-enhanced fluorescence: an emerging tool in biotechnology, Aslan, K., Gryczinski, I., Malicka, J., Matveeva, E. Lakowicz, J. ,and Geddes, C.D. (2005) Current Opinion in Biotechnology, 16:1-8

Fast and Slow Deposition of Silver Nanorods on Planar Surfaces: Application to Metal-Enhanced Fluorescence, Aslan, K., Leonenko, Z., Lakowicz. J., and Geddes, C.D. (2005). J. Phys. Chemistry, 109: 3157-3162.

Enhanced Fluorescence cyanide detection at physiologically lethal levels: Reduced ICT-based signal transduction, Badugu, R., Lakowicz, J., and Geddes, C.D. (2005). J. Am. Chem.Society, 127: 3635-3641.

Rapid depositing of triangular silver nanoplates on planar surfaces: Application to metal-enhanced fluorescence, Aslan, K., Lakowicz, J., and Geddes, C.D. (2005). J. Phys. Chem. B, 109: 6247-6251.

A glucose-sensing contact lens: from bench top to patient, Badugu, R., Lakowicz, J., and Geddes, C.D. (2005). Current Opinion in Biotechnology, 16: 100-107.

Fluorescence sensors for monosaccharides based on the 6-methylquinolinium nucleus and boronic acid moiety: potential application to ophthalmic diagnostics, Badugu, R., Lakowicz, J., and Geddes, C.D. (2005). Talanta, 65: 762-768.

Enhanced lanthanide luminescence using silver nanostructures: Opportunities for a new class of probes with exceptional spectral characteristics, Wu, M., Lakowicz, J., and Geddes, C.D.(2005) Journal of Fluorescence, 15: 53-59.

Enhanced ratiometric pH sensing using SNAFL-2 on silver island films- metal-enhanced fluorescence sensing, Aslan, K., Lakowicz, J., Szmacinski, H., and Geddes, C.D. (2005). Journal of Fluorescence, 15: 37-40.

Radiative decay engineering 5: metal-enhanced fluorescence and plasmon emission, Lakowicz, J. (2005). Analytical Biochemistry, 337: 171-194.

Surface-plasmon-coupled emission of quantum dots, Gryczinski, I., Malicka, J., Jiang, W., Fischer, H., Chan, W., Gryczinski, Z., Grudzinski, W., and Lakowicz, J. (2005). J. Phys.Chem. B, 109: 1088-1093.

Wavelength-ratiometric near-physiological pH sensors based on 6-aminoquinolinium boronic acid probes, Badugu, R., Lakowicz, J. R., and Geddes, C. D. (2005). Talanta 66:569-574.

Enhanced luminescence of phenyl-phenanthridine dye on aggregated small silver nanoparticles, Zhang, J. and Lakowicz, J. R. (2005). J. Phys. Chem. B, 109:8701-8706.

Boronic acid fluorescent sensors for monosaccharide signaling based on the 6-methoxyquinolinium heterocyclic nucleus: progress toward noninvasive and continuous glucose monitoring, Badugu, R., Lakowicz, J., and Geddes, C.D. (2005). Bioorganic and Medicinal Chemistry, 13: 113-119.

Metal-enhanced Fluoroimmunoassay on a Silver Film by Vapor Deposition, Zhang, J., Matveeva, E., Gryczynski, I., Leonenko, Z., and Lakowicz, J. R. (2005). J. Phys. Chem. B 109:7969-7975.

Surface-enhanced Fluorescence of Fluorescein-Labeled Oligonucleotides Capped on Silver Nanoparticles, Zhang, J., Malicka, J., Gryczynski, I., and Lakowicz, J. R. (2005). J. Phys. Chem. B. 109:7643-7648.

Metal-enhanced fluorescence from plastic substrates, Aslan, K., Badugu, R., Lakowicz, J. R., and Geddes, C. (2005). J. Fluorescence 15(2):99-104.

Anion sensing using quinolinium based boronic acid probes, Badugu, R., Lakowicz, J. R., and Geddes, C. D. (2005). Current Analytical Chemistry 1:157-170.

Metal-enhanced fluorescence using anisotropic silver nanostructures: critical progress to date, Aslan, K., Lakowicz, J. R., and Geddes, C. D. (2005). Anal. Bioanal. Chem. 382:926-933.

Myoglobin immunoassay based on metal particle-enhanced fluorescence, Matveeva, E. G., Gryczynski, Z., and Lakowicz, J. R. (2005). J. Immunol. Methods 302:26-35.

Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing, Aslan, K., Holley, P., Davies, L., Lakowicz, J. R., and Geddes, C. D. (2005). J. Am. Chem. Soc. 127:12115-12121.

Plasmonic technology: Novel approach to ultrasensitive immunoassays, Lakowicz, J. R., Malicka, J., Matveeva, E., Gryczynski, I., and Gryczynski, Z. (2005). Clin. Chem. 51:1914-1922.