To elucidate post-receptor signaling that results in bile acid-induced colon cancer cell proliferation, we use cell culture, immunoblotting, radioligand binding, cell growth, cytotoxicity and apoptosis assays to determine the molecular mechanisms underlying bile acid effects in vitro on human colon cancer cell lines and in vivo on transgenic mouse intestine.
Raufman, JP., Cheng, K. & Zimniak, P. (2003) Activation of muscarinic receptor signaling by bile acids: physiological and medical implications. Dig. Dis. Sci., 48 (8),1431-1444.
Cheng, K., Zimniak, P. & Raufman, JP. (2003) Transactivation of the epidermal growth factor receptor mediates cholinergic agonist-induced proliferation of H508 human colon cancer cells. Cancer Research, 63,6744-6750.
Karaolis, D., Cheng, K., Lipsky, M., Elnabawi, A., Catalano, J., Hyodo, M., Hayakawa,Y., Raufman, JP. (2005) “3',5'-cyclic diguanylic acid inhibits basal and growth factor-stimulated human colon cancer cell proliferation. Biochemical and Biophysical Research Communications, 329, 40-45.
Cheng, K., & Raufman, JP. (2005) Bile acid-induced proliferation of a human colon cancer cell line is mediated by transactivation of epidermal growth factor receptors. Biochemical Pharmacology, 70, 1035-1047.