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James P Carney Ph.D.
Academic Title: Adjunct Assistant Professor
Primary Appointment: Radiation Oncology
Location: Off-Campus
Phone: (410) 436-5598
Fax: (410) 436-6536

Personal History

Education

Ph.D., Molecular and Cellular Biochemistry, Loyola University Medical Center, 1994

B.S., Chemistry/Education, Niagara University, Niagara University, NY, 1989

Research and Professional Experience

2/09-Present: Research Biologist, Biosciences Division, Edgewood Chemical Biological Center, US Army, Aberdeen Proving Ground.

11/07-Present: Senior Research Scientist, Battelle Memorial Institute in support of contract at the Edgewood Chemical Biological Center. Provided support to the Molecular Engineering Branch in the Biosciences Division.

7/99 – 7/07: Assistant Professor, Radiation Oncology Research Laboratory, Department of Radiation Oncology, Member, Molecular and Cell Biology Graduate Program and Member, Mary and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore Maryland.

10/96 – 6/99: Postdoctoral Fellow in the laboratory of Dr. William F. Morgan at the University of California, San Francisco and Lawrence Berkeley National Laboratory.

11/94 - 10/96: Postdoctoral Fellow in the laboratory of Dr. Stuart Linn, Department of Molecular and Cellular Biology, University of California, Berkeley, California.

7/90 - 10/94: Graduate Fellow in the laboratory of Dr. Mark R. Kelley, Department of Molecular and Cellular Biochemistry, Loyola University Medical School, Maywood, Illinois.

8/89 - 6/90: Graduate student at the Akron University in the laboratory of Dr. David Perry, Department of Chemistry.

Patents and Licenses:

Patents and Licenses

Patent number 7,074,902: “Antibody specific for a DNA repair protein”, 2006.

Licensed Rad50 hook antiserum to Novus Biologicals, catalog number NB 100-1487, 2006.

Publications

Abuzeid, W.M., Jiang, J., Shi, G., Wang, H., Paulson, D., Araki, K., Jungreis, D., Carney, J., O’Malley Jr., B.W., and Li, D. (2009) Molecular disruption of Rad50 sensitizes human tumor cells to cisplatin-based chemotherapy. J. Clinical Investigation. In Press.

Williams, R.S., Moncalian, G., Williams, J.S.,Yamada, Y., Limbo, O., Shin,D.S., Groocock,L.M., Cahill, D., Hitomi,C., Guenther, G., Moiani, D., Carney, J.P., Russell, P., and Tainer, J.A. (2008) Mre11 Dimers Coordinate DNA End Bridging and Nuclease Processing in Double-Strand-Break Repair. Cell. 135: 97-109

McDowell, H.D., Carney, J.P. and Wilson, T.M. (2008) Inhibition of the 5′ to 3′ exonuclease activity of hExo1 by 8-oxoguanine. Environmental and Molecular Mutagenesis. 49:388-98.

Cahill D. and Carney J.P. (2007) Dimerization of the Rad50 protein is independent of the conserved hook domain. Mutagenesis. 22:269-74.

Rhee, J.G., Li, D., Suntharalingam, M., Guo, C., O’Malley, B.W. Jr. and Carney, J.P. (2006) Radiosensitization of head/neck sqaumous cell carcinoma by adenovirus-mediated expression of the Nbs1 protein. International Journal of Radiation Oncology, Biology, Physics. 67:273-8.

Gueven N., Becherel O.J., Birrell G., Chen P., DelSal G., Carney J.P., Grattan-Smith P., Lavin M.F. (2006) Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype. Cancer Research. 66:2907-12.

Cahill, D., Connor, B.K. and Carney J.P. (2006) Mechanisms of DNA Double-Strand Break Repair. Frontiers in Bioscience. 11:1958-76.

Cheng W.H., Sakamoto S., Fox J.T., Komatsu K., Carney J., Bohr V.A. (2005) Werner syndrome protein associates with gamma H2AX in a manner that depends upon Nbs1. FEBS Letters. 579:1350-6.

DeJager, M., Trujillo, K., Sung, P., Hopfner, K-P., Carney, J.P., Tainer, J.A., Connelly, J.C., Leach D.R.F., Haering, C., Nasmyth, K., Kannaar, R. and Wyman, C. (2004) Differential arrangement of conserved building blocks among homologs of the Rad50/Mre11 DNA repair complex. Journal of Molecular Biology. 339: 937-949.

Cheng, W-H., von Kobbe, C., Opresko,P.L., Arthur, L.M., Komatsu, K., Seidman, M.M., Carney, J.P. and Bohr, V.A. (2004) Linkage between Werner syndrome protein and the Mre11 complex via Nbs1. Journal of Biological Chemistry. 279: 21169-21176.

Arthur, L.M., Gustausson, K., Hopfner, K-P., Carson, C.T., Stracker, T.H., Karcher, A., Felton, D., Weitzman, M.D., Tainer, J.A. and Carney, J.P. (2004) Structural and Functional Analysis of Mre11-3. Nucleic Acids Research. 32: 1886-1893.

Manzan, A., Pfeiffer, G., Hefferin, M.L., Lang, C.E., Carney, J.P. and Hopfner, K-P. (2004) MlaA, a hexameric ATPase linked to the Mre11 complex in archaeal genomes. EMBO Reports 5:54–59

Tran H.M., Shi G., Li G., Carney J.P., O'Malley B., Li D. (2004) Mutant Nbs1 enhances cisplatin-induced DNA damage and cytotoxicity in head and neck cancer. Otolaryngology Head Neck Surgery. 131:477-84.

O'Malley B.W. Jr, Li D., Carney J., Rhee J., and Suntharalingam M. (2003) Molecular disruption of the MRN(95) complex induces radiation sensitivity in head and neck cancer. Laryngoscope 113: 1588-94.

Hopfner, K.P., Craig, L., Moncalian, G., Zinkel, R.A., Takehiko, U., Owen, B.L., Karcher, A., Henderson, B., Bodmer, J-L., McMurray, C.T., Carney, J.P., Petrini, J.H.J. and Tainer, J.A. (2002) Rad50 Zn hook: a novel structure joining Mre11 complexes in DNA recombination and repair. Nature 418: 562-6.

Hopfner, K.P., Karcher, A., Craig, L., Woo,T.T., Carney, J.P. and Tainer, J.A. (2001) Structural biochemistry and interaction architecture of the DNA double-strand break repair Mre11 nuclease and Rad50 ATPase. Cell 105:473-485.

Hopfner, K.P., Karcher, A., Fairley, C., Tainer, J.A. and Carney, J.P. (2000) Mre11 and Rad50 from Pyrococcus furiosus: Cloning and biochemical characterization reveal an evolutionarily conserved multiprotein machine. J. Bacteriology 182:6036-6041.

Hopfner, K-P., Karcher, A., Shin, D.S., Craig, L., Arthur, L.M., Carney, J.P. and Tainer, J.A. (2000) Structural biochemistry of Rad50 ATPase: ATP-driven cooperativity and allosteric control in DNA double-strand break repair and the ABC ATPase superfamily. Cell 101: 789-800.

Carney, J.P. (1999) Chromosomal breakage syndromes. Current Opinion in Immunology. 11: 443-447.

Carney, J.P. and Morgan, W.F. (1998) Induction of DNA double-strand breaks by electroporation of restriction enzymes into mammalian cells. In DNA Repair Protocols, 465-472. Edited by Daryl S. Henderson. Humana Press, Totowa, NJ.

Wilson, D.M., Carney, J.P., Coleman, M.A., Adamson, A.W., Christensen, M., Lamerdin, J. (1998) Hex1: a new human RAD2 nuclease family member with homology to yeast exonuclease 1. Nucleic Acids Research. 26: 3762-68.

Carney, J.P., Maser, R., Olivares, H., Davis, E.M., Le Beau, M., Yates, J.R.III, Hays, L., Morgan, W.F., and Petrini, J.H.J. (1998) The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: Linkage of double-strand break repair to the cellular DNA damage response. Cell 93: 477-486.

Maze, R., Carney, J.P., Kelley, M.R., Glassner, B., Williams, D.A., Samson, L. (1996) Increased DNA alkylation repair via bone marrow stem cell transduction rescues mice from the toxic effects of a chemotherapeutic alkylating agent. Proc. Nat. Acad. Sci. USA 93: 206-210.

Carney, J.P., McKnight, C., Van Epps, S., and Kelley, M.R. (1995) Random rapid amplification of cDNA ends (RACE) allows for cloning of multiple novel human cDNA fragments containing (CAG)N repeats. Gene 155: 289-292.

Wilson, T.M., Carney, J.P., and Kelley, M.R. (1994) Cloning of the multifunctional rat apurinic/apyrimidinic endonuclease (rAPEN)/redox factor from an immature T cell line. Nucleic Acids Res. 22: 530-531.

Wilson, D.M., III, Tentler, J.T., Carney, J.P., Wilson, T.M., and Kelley, M.R. (1994) Acute ethanol exposure suppresses the repair of O6-methylguanine DNA lesions in castrated adult rats. Alcoholism: Clin. and Exp. Res. 18: 1267-1271.

Grabowski, D.T., Carney, J.P., and Kelley, M.R. (1991) An adult male specific gene in Drosophila containing the repetitive element opa. Biochem. Biophys. Acta 1090: 115-118.

Grabowski, D.T., Carney, J. P., and Kelley, M.R. (1991) A Drosophila gene containing the opa repetitive element is exclusively expressed in adult male abdomens. Nucleic Acids Res. 19: 1709.