Gary  M Fiskum
 

Gary M Fiskum Ph.D.

Academic Title: Professor
Primary Appointment: Anesthesiology
Secondary Appointments: Biochemistry and Molecular Biology, Pharmacology and Experimental Therapeutics
Administrative Title: Vice Chair
gfiskum@anes.umm.edu
Location: MSTF 534
Phone: (410) 706-4711
Phone: (410) 706-3418
Fax: (410) 706-2550
Lab: (410) 706-2990

Personal History

EDUCATION/TRAINING
 
  • BA, Zoology, UCLA
  • PhD, Biochemistry, St. Louis University
  • Postdoctoral, Biochemistry, Johns Hopkins Sch. Med.
FACULTY POSITIONS
 
  • 09/81: Asst. Professor of Biochemistry, George Washington Univ. School of Medicine
  • 07/87: Tenured Associate Professor of Biochemistry and of Emergency Medicine, GWUMC
  • 07/91: Professor of Biochemistry and Molecular Biology and of Emergency Medicine, GWUMC
  • 10/97: Professor and Research Director of Anesthesiology, Professor of Biochemistry and Molecular Biology Professor of Pharmacology and Experimental Therapeutics, Univ. of Maryland School of Medicine
  • 03/03: Vice-Chair for Research, Department of Anesthesiology, Univ. of Maryland School of Medicine 
RECENT HONORS
 
  • Keynote Speaker - 2002 Conference on Cellular Signaling in Neuroprotection and Plasticity in Germany
  • Keynote Speaker - 2002 meeting of the Society of Neurosurgical Anesthesia and Critical Care in Florida 
RECENT FEDERAL GOVERNMENT PUBLIC ADVISORY COMMITTEES
 
  • NIH NINDS Special Emphasis Panel for U01 Cooperative Program in Translational Research
  • NIH NIA for P01 Application
  • NIH NHLB Stroke RFA Special Emphasis Panel
  • NIH CSR NDBG Ad hoc
  • Veterans Administration Scientific Review of Combat Neurotrauma ('06, '07)
  • NIH CSR MDCN-B (Ad hoc '07)

Research Interests

  • Molecular mechanisms of ischemic and traumatic brain injury
  • Clinically relevant animal models of brain injury caused by cardiac arrest
  • Neonatal asphyxia
  • Blunt head trauma to adults and children
  • Penetrating head trauma and explosions (blasts)
  • Development of neuroprotective interventions for acute brain injury
  • Mitochondrial bioenergetics and cell biology
  • Free radical biology and medicine
  • Intracellular Ca2+ homeostasis
  • Mechanisms of apoptotic and necrotic cell death

Lab Techniques and Equipment

  • Clinically relevant small and large animal models of acute brain injury
  • Respirometry for isolated mitochondria and cells
  • Primary culture of neurons and astrocytes using an environmental chamber precisely controling ambient oxygen concentrations
  • Fluorescent measurements of cell death
  • Development of transgenic animals
  • Fluorescent live cell imaging of intracellular NAD(P)H, calcium, mitochondrial membrane potential and superoxide
  • Stereologic histology and immunohistochemistry
  • 13C NMR spectroscopy of brain energy metabolites
  • Morris water maze analysis of rodent learning and memory
  • Enzyme activity measurements rtPCR
  • Southern and Western blots
  • ELISA Spectrofluorometry

Publications

Selected Publications

Rosenthal, R.E. and Fiskum, G., Oxygen: Could there be too much of a good thing? Hosp Med. 66: 76-77 (2005).

Hainsworth, A.H., Bermpohl, D., Webb, T.E., Darwish, R., Fiskum, G., Qiu, J., McCarthy, D., Moskowitz, M.A., Whalen, M.J., Expression of cellular FLICE inhibitory proteins (cFLIP) in normal and traumatic murine and human cerebral cortex, J. Cereb. Blood Flow Metab. 25: 1030-1040 (2005).

Chinopoulos,C., Starkov, A.A., Grigoriev, S., Dejean, L.M., Kinnally, K.W., Liu, X., Ambudkar, I.S., Fiskum, G., Diacylglycerols activate mitochondrial cationic channel(s) and release sequestered Ca(2+), J. Bioenerg. Biomemb. 37: 237-247 (2005).

Mehrabian, Z., Liu, L-I., Fiskum, G., Rapoport, S.I., and Chandrasekaran, K., Regulation of mitochondrial gene expression by energy demand in neural cells, J. Neurochem. 93: 850-860 (2005).

Soane, L. and Fiskum, G., Inhibition of mitochondrial neural cell death pathways by protein transduction of bcl-2 family proteins, J. Bioenerg. Biomemb. 37: 179-180 (2005).

Soane, L. and Fiskum, G., TAT-mediated endocytotic delivery of the loop deletion Bcl-2 protein protects neurons against cell death, J. Neurochem. 95: 230-243 (2005).

Schuh, R.A., Kristian, T., Gupta, R.K., Flaws, J.A., and Fiskum, G., Methoxychlor inhibits brain mitochondrial respiration and increases hydrogen peroxide production and CREB phosphorylation, Toxicol Sci. 88: 495-504 (2005).

Robertson, C.L., Puskar, A., Hoffman, G.E., Murphy, A.Z., Saraswati, M., and Fiskum G., Physiologic progesterone reduces mitochondrial dysfunction and hippocampal cell loss after traumatic brain injury in female rats, Exp. Neurol., 197:235-243 (2006)

Richards, E.M., Rosenthal, R.E., Kristian, T., and Fiskum, G., Postischemic hyperoxia reduces hippocampal pyruvate dehydrogenase activity, Free Rad. Biol. Med. 40: 1960-1970 (2006)

Vereczki, V., Martin, E., Rosenthal, R.E., Hof, P.R., Hoffman, G.E., and Fiskum, G., Normoxic resuscitation after cardiac arrest protects against hippocampal oxidative stress, metabolic dysfunction, and neuronal death, J. Cereb. Blood Flow Metab., 26: 821-35 (2006).

Bambrick, L., Wright, C., and Fiskum, G., Cyclosporin A increases mitochondrial calcium uptake capacity in cortical astrocytes but not cerebellar granule neurons, J. Bioenerg. Biomemb. 38:43-47 (2006).

Balan, I.S., Fiskum, G., Hazelton, J., Cotto-Cumba, C., and Rosenthal, R.E., Oximetry-guided reoxygenation improves neurological outcome after experimental cardiac arrest, Stroke 37: 3008-3013 (2006).

Chandrasekaran, K., Hazelton, J., Wang, Y., Fiskum, G., and Kristian, T., Neuron-specific conditional expression of a mitochondrially targeted fluorescent protein in mice, J. Neurosci. 26: 13123-13127 (2006).

Kahraman, S. and Fiskum, G., Anoxia-induced changes in pyridine nucleotide redox state in cortical neurons and astrocytes, Neurochem. Res. 32: 799-806 (2007).

Mehrabian, Z., Chandrasekaran, K., Kristian, T., Fiskum, G., and Kalvakolanu, D.B., The interferon β and retinoic acid induced cell death regulator GRIM-19 are upregulated during focal cerebral ischemia, J. Interferon Cytokine Res. 27: 383-392 (2007).

Richards, E.M., Fiskum, G., Rosenthal, R.E., Hopkins, I., and McKenna, M.C., Hyperoxic reperfusion following global ischemia decreases hippocampal energy metabolism, Stroke. 38: 1578-1584 (2007).

Robertson, C.L., Saraswati, M., and Fiskum, G., Mitochondrial dysfunction early after traumatic brain injury in immature rats, J. Neurochem. 101: 1248-1257 (2007).

Carlson, D.E., Nguyen, P.X., Soane, L., Fiedler, S.M., Fiskum, G., Chiu, W.C., Scalea, T.M. , Hypotensive hemorrhage increases calcium uptake capacity and Bcl-XL content of liver mitochondria. Shock. 27: 192-198 (2007).

Kristian, T., Pivovarova, N.B., Fiskum, G., Andrews, S.B., Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. J. Neurochem. 102:1346-1356 (2007)




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