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James B Wade
Ph.D.
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| Academic Title:
Professor |
| Primary Appointment:
Physiology |
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jwade@umaryland.edu |
| Location:
655 West Baltimore St.
BRB 5-039 |
| Phone:
410-706-3190 |
| Fax:
410-706-8341 |
| Lab:
410-706-7974 |
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Personal History
Ph.D - Princeton University (1972)
Research Interests
Research in my laboratory is oriented toward understanding the cellular and molecular mechanisms involved in the signals mediating hormonal regulation of transport by kidney epithelial cells. Hormonal regulation of epithelial transport is essential for appropriate control of the volume and composition of body fluids. Antidiuretic hormone (ADH) and aldosterone play a central role in this control by regulating sodium and water transport by certain epithelial cells in the kidney. ADH increases the water permeability of responsive epithelia by causing vesicles containing water channels to fuse with the cell's apical membrane. ADH also regulates concentrating ability by regulating a family of urea transporters. Aldosterone regulates the amount of the epithelial Na channel ENaC, the NaCl cotransporter, NCC and the K channel, ROMK in the apical membrane. We have been studying the role of membrane vesicle traffic in these responses using specific antibodies to immunolocalize transporters and PDZ proteins involved in the targeting and regulation of membrane transporters. We are also interested in signaling via serine/threonine kinases WNK1 especially WNK1 and WNK4 which are involved in regulating blood pressure and K balance.
Lab Techniques and Equipment
Immunocytochemistry, Confocal and Electron Microscopy, Western immunoblots, RT-PCR, oocyte expression studies.
- Immunocytochemistry
- Confocal Microscopy
- Frozen and Ultrathin Sectioning
- Electron Microscopy
- Western immunoblots
Laboratory Personnel:
- Richard A. Coleman, PhD
- Jie Liu
Publications
1. Shenolikar, S., Voltz, J.W., Minkoff, C.M., Wade, J.B. and Weinman, E.J. (2002) Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wasting. Proc Natl Acad Sci U. S A. 99:11470-5.
2. Wade, J.B., Liu, J., Coleman, R.A., Cunningham, R., Steplock, D.A., Lee-Kwon, W., Pallone, T.L., Shenolikar, S., Weinman, E.J. (2003) Localization and interaction of NHERF isoforms in the renal proximal tubule of the mouse. Am J Physiol Cell Physiol. 285:C1494-C1503.
3. Weinman, E.J., Boddeti, A., Cunningham, R., Akom, M., Wang, F., Wang, Y., Liu, J., Steplock, D., Shenolikar, S., Wade, J.B. (2003) NHERF-1 is Required for Renal Adaptation to a Low Phosphate Diet. Am J Physiol Renal Physiol. 285:F1225-F1232.
4. Spector, D.A., Yang, Q., Liu, J., Wade, J.B. (2004 Jul) Expression, localization, and regulation of urea transporter B in rat urothelia. Am J Physiol Renal Physiol. 287(1):F102-108.
5. Olsen, O., Wade, J.B., Morin, N., Bredt, D.S., Welling, P.A. (2005) Differential localization of the Mammalian Lin 7 (MALS/Veli) PDZ Proteins in the Kidney. Am J Physiol Renal Physiol.; 288: F345-F352.
6. Khan, O., Riazi, S., Hu, X., Song, J., Wade, J.B., and Ecelbarger, C.A. (2005) Regulation of the Renal Thiazide-Sensitive Na-Cl Cotransporter, Blood Pressure, and Natriuresis in Obese Zucker Rats Treated with Rosiglitazone. Am J Physiol Renal Physiol 289(2):F442-50.
7. Coleman, R.A., Liu, J., Wade, J.B. (2006) Use of Anti-fluorophore Antibody to Achieve High-Sensitivity Immunolocalizations of Transporters and Ion Channels. J Histochem Cytochem. 54(7):817-27.
8. Wade, J.B., Fang, L., Liu, J., Li, D., Yang, C-L., Subramanya, A.R., Maouyo, D., Mason, A., Ellison, D.H., and Welling, P.A. (2006) WNK1 Kinase Isoform Switch Regulates Renal Potassium Excretion. Proc. Natl. Acad. Sci. U.S.A. 103(22):8558-63.
Faculty members:
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