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Dhan V Kalvakolanu
 

Dhan V Kalvakolanu Ph.D.

Academic Title: Professor
Primary Appointment: Microbiology and Immunology
dkalvako@umaryland.edu
Location: Howard Hall, 350
Phone: (410) 328-1396 (office)
Phone: (410) 328-1398 (lab)
Fax: (410) 706-6609

Personal History:

1979: BS (Biology & Chemistry), Osmania University, Hyderabad, India

1981: MS (Microbiology), Osmania University, Hyderabad, India

1988: Ph.D (Microbiology), Indian Institute of Science, Bangalore, India

Research Interests:

Regulation of gene transcription and signal transduction by cytokines. e.g. JAK-STAT singaling, MAPK-singaling C/EBP, AP1 and ATF singaling pathways.
Tumor cell growth control, Apoptosis and the regulation of novel Cell death-activating genes.

Lab Techniques and Equipment:

The PI has published several papers that employed all standard molecular biological techniques including gene isolation, transfection, mutational analysis, recombinant protein expression, generation of monoclonal antibodies, protein:protein interactions, cell growth and apoptosis, DNA binding assays such as in vivo foot printing, EMSA, ChIP assays, Nuclear run off transcription assays, RNAi, reconsitution of gene expression in mammalian cells following RNAI, lentiviral gene expression systems, Mass spectrometry, and knockout cell lines and kncokout mice. All oncogenic assays- such as matrigel and soft-agar growth assays, cell migration,tumor xenograft models etc.


Publications:

Kalakonda, S., Nallar, S.C., Lindner, D.J., Hu, J., Reddy, S.P. and Kalvakolanu, D.V. (2007) Tumor suppressive activation of the cell death activator GRIM-19 on a constitutively active STAT3. Cancer Res 67: 6212-20

Zhang, L., Zhao, L., Guo, B., Ji, K., Tian, Y., Wang, J., Yu, H., Hu, J., Kalvakolanu, D.V., Kopecko, D.J., Zhao, X., and Xu, D-Q (2007) Intratumoral delivery and suppression of prostate tumor growth by attenuated Salmonella enterica serovar Typhimurium carrying plasmid based shRNAs. Cancer Res 67: 5859-5864.

Alchanati, I., Nallar, S.C., Sun, P., Gao, L., Hu, J., Stein, A., Yakirevich, E., Konforty, D., Alroy, I., Zhao, X., Reddy, S.P., Resnick, M.B., and Kalvakolanu, D.V. (2006) A proteomic analysis reveals the loss of expression of the cell death regulatory gene GRIM-19 in human renal cell carcinomas. Oncogene 25:7138-7147.

Roy, S. K., Shuman, J. D., Platanias, L. C., Shapiro, P. S., Reddy, S. P., Johnson, P. F., and Kalvakolanu, D. V. (2005). A Role for Mixed Lineage Kinases in Regulating Transcription Factor CCAAT/Enhancer-binding Protein-{beta}-dependent Gene Expression in Response to Interferon-{gamma}. J Biol Chem 280, 24462-2447.

Meng, Q., Raha, A., Roy, S., Hu, J., and Kalvakolanu, D. V. (2005). IFN-gamma-stimulated transcriptional activation by IFN-gamma-activated transcriptional element-binding factor 1 occurs via an inducible interaction with CAAAT/enhancer-binding protein-beta. J Immunol 174, 6203-6211.

Zhang, J., Yang J., Roy, S.K., Tininini, S., Hu, J., Bromberg, J.F., Poli, V., Stark, G.R., and Kalvakolanu, D.V. (2003) The cell death regulator GRIM-19 is an inhibitor of signal transducer and activator of transcription 3. Proc. Natl. Acad. Sci. USA 100: 9342-9347.

Roy, S.K., Hu, J., Meng, Q, Xia,Y., Shapiro, P.S., Reddy, S.P.M., Platanias, L.C., Lindner, D. J., Johnson, P.F., Pritchard, C., Pages G., Pouyssegur, J. and Kalvakolanu, D. V. (2002) MEKK1 plays a critical role in activating transcription factor C/EBP-b dependent gene expression in response to IFN-g.
Proc. Natl.
Acad. Sci. USA
99:7945-7950.

Seo, T., Lee D., Shim, Y.S., Angell, J.E., Chidambaram, N.V., Kalvakolanu, D.V. and Choe, J. (2002) Viral Interferon regulatory factor 1 of Kaposiâ?Ts Sarcoma-Associated Herpes Virus interacts with a cell death regulator, GRIM-19, and inhibits Interferon-retinoic acid-induced cell death. J. Virol. 76: 8797-8807.

Joshi, V.D., Kalvakolanu, D.V., Hebel, R.J., Hasday, J.D., and Cross, A.S. (2002) Caspase 1 is essential for host defense against gram-negative bacterial infections. Infection & Immunity. 70:6896-6903.