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Dan H. Schulze

Dan H. Schulze Ph.D.

Academic Title: Professor
Primary Appointment: Microbiology and Immunology
Secondary Appointments: Otorhinolaryngology-Head & Neck Surgery
Location: BRB 3-031
Phone: (410) 706-4762
Phone: (410) 706-5180
Fax: (410) 706-2129

Personal History:

Indiana University, B.A., 1969, (Zoology. T.G. Gregg, Advisor); Miami University, 1972 M. S. (Zoology/Genetics); University of Texas, Ph.D. 1977 (Zoology, C.S. Lee, Advisor); University of Minnesota (St. Paul) 1978 Postdoctoral fellow (Cell Biology Walter Sauerbier, Advisor); Albert Einstein College of Medicine 1981 Postdoctoral Fellow (Immunology S. Nathenson, Advisor); University of Texas Medical Branch-Galveston 1984 Assistant Professor (Department of Microbiology and Immunology); 1989-present, University of Maryland School of Medicine (Department of Microbiology and Immunology)

Research Interests:

Having been trained in molecular biology and evolution my research interests have taken root in two different areas: first, the study of questions related to how the adaptive immune system recognizes and responds to pathogens, second, the study of membrane associated ion transporters that regulate cellular response.

In Studying Antibodies
After my experiences in studying diversity generation mechanisms during my postdoc, I became interested in antibodies. I initially addressed questions of antibody repertoire development using both in vivo and in vitro approaches. Recently I have used my experience working with antibodies to construct chimeric antigen receptor (CARs) to be expressed in T cells. I am interested in developing the use of CAR constructs expressed in T cells not only to activate but to block interactions between tumor cells and the immune system that normally would result in inhibition of T cell function within the tumor environment. I am currently collaborating with another investigator in the department to determine if CARs expressed in a specific subset of T cells, NKT cells may prove more robust in treating tumors and change the current paradigm in cancer immunotherapy. Another use of antibody specificity can be used to develop biosensors to expand the ability to test for the presence of pathogens in the environment. I have engineered a construct that is composed of a molecule that actively binds antibodies fused to the transmembrane and to a signaling/activation domain used to activate B cells. This universal biosensor platform provides a rapid way to expand the testing capabilities by using commercially available monoclonal antibodies.

Cellular and Molecular Ca2+ Signaling-the Sodium Calcium Exchanger.
After moving to Maryland I became interested in characterization of membrane transport molecules that regulate calcium (Ca) in cells. Specifically, we initially studied the sodium/calcium (Na/Ca) exchanger in mammalian species, and Na/Ca exchanger genes in Drosophila and bacteria. We initially discovered that the Na/Ca exchanger RNA undergoes alternative splicing producing tissue-specific exchanger proteins that differ in the regulation of function in various cell types. We are studying the mechanism that provides specificity for RNA splicing and we are also studying how these alternatively spliced forms of the protein alter NCX function in expression systems. Finally, the laboratory is interested in the evolutionary aspects of the Na/Ca exchanger and related Ca ion transporters. Study of different transport molecules for this gene family will provide insight into structure and function of ion transporters.


Jain A, Poonia B, So EC, Vyzasatya R, Burch EE, Olsen HS, Mérigeon EY, Block DS, Zhang X, Schulze DH, Hanna NN, Twadell WS, Yfantis HG, Chan SL, Cai L, Strome SE. Tumour antigen targeted monoclonal antibodies incorporating a novel multimerisation domain significantly enhance antibody dependent cellular cytotoxicity against colon cancer. Eur. J. Cancer 49:3344-52 2013 (PMID:23871153)
Jain, A, Olsen, H.S,Vyzasatya, R., Burch, E.,Sakoda, Y. Merigeon, E.Y., Cai, L., Lu, C, Tan, M., Tamada, K. Schulze D.H., Block, D.S, and Strome S.E. Fully recombinant IgG2a Fc multimers (stradomers) effectively treat collagen-induced arthritis and prevent idiopathic thrombocytopenic purpura in mice. Arthritis Res. Ther. R192 2012 (PMID:22906120)

Giladi M, Bohbot H, Buki T, Schulze DH, Hiller, R. Khananshvilli D. Dynamic Features of Allosteric Ca2+ Sensor in Tissue-Specific NCX Variants. Cell Calcium 51:478-485, 2012 (PMID:22571864)

Taylor R.J., Chan S-L., Wood A., VoskensC.J., Wolf J.S., Wei, L., Chapoval, A., Schulze D.H., Cullen, K., Strome S.E. Fc¿RIIIa polymorphisms and cetuximab induced cytotoxicity in squamous cell carcinoma of the head and neck. Cancer Immunol. Immunother 58:997-1006, 2009 (PMID: 18979096).

Lin W, Voskens CJ, Zhang X, Schindler DG, Wood A, Burch E, Wei Y, Chen L, Tian G, Tamada K, Wang LX, Schulze DH, Mann D, Strome SE. Fc-dependent expression of CD137 on human NK cells: insights into "agonistic" effects of anti-CD137 monoclonal antibodies. Blood 112:699-707, 2008.

Kofuji, P., W.J. Lederer and D.H. Schulze. Mutually exclusive and cassett exons underlie alternately spliced isoforms of the Na+/Ca2+ exchanger. J. Biol. Chem. 269:5145-5149, 1994 (PMID: 8106495).

Schulze DH, Mughal M, Lederer WJ, Ruknudin AM. Sodium/calcium exchanger (NCX1) macromolecular complex. J. Biol Chem. 278:28549-28555, 2003.