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John E Jones
 

John E Jones Ph.D.

Academic Title: Associate Professor
Primary Appointment: Medicine
jjones2@medicine.umaryland.edu
Location: HSFII, S003C
Phone: (410) 706-5596
Fax: (410) 706-6034

Personal History:

Essential hypertension is a complex, multifactorial disease arising from the interaction of genetics and environmental influences. Although a great deal has been learned, we have yet to elucidate the molecular mechanisms of this disease. Our goal is to determine the molecular pathways involved in the modulation of blood pressure and to develop model systems that facilitate the study of those pathways.

Traditional transgenic model systems of human disease may be compromised by compensatory changes in gene expression that arise during development. Moreover, inappropriate temporal or spatial expression of the gene under investigation is likely to limit the value of the model.

Dr. Jones has extensive experience in the regulation of gene expression and has incorporated this expertise in the design and development of molecular tools that allow us to overcome many of the limitations of traditional transgenic systems. Dr. Jones has developed several model systems for the study of important genes in human hypertension. These systems incorporate single-copy, targeted genomic integration of the gene of interest and allow us to exert spatial and temporal control over its expression.

Dr. Jones also has considerable interest in elaborating the molecular mechanism of blood pressure regulation. He has identified several novel protein interacting partners of the D1 and D3 dopamine receptors—proteins that are known to play significant roles in blood pressure regulation by modulating water and salt homeostasis. The identification of sorting nexin 5 (Snx5) as an Interacting partner of the D1 dopamine receptor, has provided considerable insight into a previously unknown, yet vitally important, mechanism of cellular trafficking of the dopamine receptor in response to an agonist.

Education and Training

  • 1977: University of Maryland - B.S., Microbiology
  • 1986: Purdue University - Ph.D., Molecular Biology & Genetics
  • 1986: National Research Council Fellow
    Laboratory of Developmental Pharmacology, National Institute of Child Health and Development, National Institutes of Health
  • 1989: Staff Fellow
    Laboratory of Developmental Pharmacology, National Institute of Child Health and Development, National Institutes of Health
  • 1991: Senior Staff Fellow
    Division of Cancer Prevention and Control, National Cancer Institute, Biomarkers and Prevention Research Branch, National Institutes of Health

Publications:

Villar VA, Armando I, Sanada H, Frazer LC, Russo CM, Notario PM, Lee H, Cominsky L, Russell HA, Yang Y, Jurgens JA, Jose PA, and Jones JE. Novel Role of Sorting Nexin 5 in Renal D1 Dopamine Receptor Trafficking and Function: Implications for Hypertension. FASEB J 2012 Nov 29. {Epub ahead of print]

Villar VA, Jones JE, Armando I, Asico LD, Escano CS, Lee H, Wang X, Yang Y, Pascua-Crusan AM, Palmes-Saloma CP, Felder RA, and Jose PA. Sorting Nexin 1 Loss Results in D5 Dopamine Receptor Dysfunction in Human Renal Proximal Tubule Cells and Hypertension in Mice. J Biol Chem. 2012 Nov14. [Epub ahead of print]

Carey RM, Schoeffel CD, Gildea JJ, Jones JE, McGrath HE, Gordon LN, Park MJ, Sobota RS, Underwood PC, Williams J, Sun B, Raby B, Lasky-Su J, Hopkins PN, Adler GK, Williams SM, Jose PA, Felder RA. Salt Sensitivity of blood pressure is associated with polymorphisms in the sodium bicarbonate cotransporter. Hypertension. 2012 Nov;60(5):1359-66

Zhang Y, Cuevas S, Asico LD, Escano C, Yang Y, Pascua AM, Wang X, Jones JE, Grandy D, Eisner G, Jose PA, Armando I. Deficient dopamine D2 receptor function causes renal inflammation independently of high blood pressure. PLoS One. 2012;7(6):e38745. Epub 2012 Jun 14.

Cuevas S, Zhang Y, Yang Y, Escano C, Asico L, Jones JE, Armando I, Jose PA. Role of renal DJ-1 in the pathogenesis of hypertension associated with increased reactive oxygen species production. Hypertension. 59(2):446-52, 2012.

Jones JE, Jurgens J, Evans S, Ennis R, Villar V, Jose PA. Mechanisms of Fetal Programming in Hypertension. (2012) International Journal of Pediatrics Volume 2012, Article ID 584831.

Sanada H, Jones JE, Jose PA. Genetics of salt-sensitive hypertension. (2011) Current Hypertension Reports. 13(1):55-66.

Asico L, Zhang X, Jiang J, Cabrera D, Escano CS, Sibley DR, Wang X, Yang Y, Mannon R, Jones JE, Armando I, Jose PA. Lack of Renal Dopamine D5 Receptors Promotes Hypertension. (2011) J Am Soc Nephrol.

Gildea JJ, Shah I, Weiss R, Casscells ND, McGrath HE, Zhang J, Jones JE, Felder RA. HK-2 human renal proximal tubule cells as a model for G protein-coupled receptor kinase type 4-mediated dopamine receptor uncoupling. (2010) Hypertension. 56(3):505-11.

Wang X, Luo Y, Escano CS, Yang Z, Asico L, Li H, Jones JE, Armando I, Lu Q, Sibley DR, Eisner GM, Jose PA. Upregulation of renal sodium transporters in D5 dopamine receptor-deficient mice. (2010) Hypertension. 55:1431-7.

Villar VA, Jones JE, Armando I, Palmes-Saloma C, Yu P, Pascua AM, Keever L, Arnaldo FB, Wang Z, Luo Y, Felder RA, Jose PA. G protein-coupled receptor kinase 4 (GRK4) regulates the phosphorylation and function of the dopamine D3 receptor. (2009) J Biol Chem. 284:21425-34.