1978: B.A., Central High School of Philadelphia
1982: B.A., University of Pennsylvania
1986: M.D., University of Pennsylvania
POST GRADUATE EDUCATION AND TRAINING
1986-1989: Internal Medicine Residen, University of Pittsburgh Presbyterian University Hospital
1989-1993: Cardiology Fellow, Medical Center Hospital and University of Vermont
Internal Medicine - 1991
Cardiovascular Medicine -1993; re-certified 2004
1993-2011: Case Western Reserve University
2011-present: University of Maryland School of Medicine
Research InterestsREGULATION OF GENE EXPRESSION AND:
1) Vascular smooth muscle adaptations to altered blood flow with a focus on myosin phosphatase (MP). The working model is that alterantive splicing of MP under the control of Transformer proteins sets the sensitivity of VSM to signals that regulate blood flow, e.g. NO/cGMP.
2) Tissue hypoxia and heart morphogenesis. We have identified tissue oxygen gradients in the developing heart and proposed that these a) are required for apoptosis-dependent remodeling and patterning of the cardiac outlet structures b) pre-dispose to congenital heart defects.
Lab Techniques and EquipmentMouse models: conditional gene knock-out, creation of new knock-out models. Surgical models: arterial ligations, hypoxic stress Molecular biology: PCR, real-time PCR, Western blot, IHC, plasmid cloning, sub-cloning and mutagenesis, RNA-protein binding assays, arrays, IP etc.
Physiology: vessel contractility in wire myograph; ECHO for cardiac structure and blood flow
Grants & Contracts:
2/10-1/14: Steven A Fisher, Smooth muscle myosin phosphatase subunit isoforms, NIH/NHLBI R01 HL66171
7/10-6/14: P.I. Steven A Fisher, Tissue hypoxia in cardiac morphogenesis, NIH/NHLBI R01 HL65314
Fu F, Mende Y, Wirth B and Fisher SA. Tra2b is required for tissue-specific splicing of a myosin phosphatase targeting subunit alternative exon in mouse fast smooth muscle tissues, 2012 May 11;287(20):16575-85. PMCID: PMC3351297
Fisher SA, Vascular smooth muscle phenotypic diversity and function, Physiol Genomics, 2010:42A: 169-187 PMCID: PMC3008361
Liu HL and Fisher SA. Hypoxia-inducible transcription factor-1a triggers an autocrine survival pathway during embryonic cardiac outflow tract remodeling, Circ Res, 2008;102(11):1331-9. PMCID: PMC2737478.
Fisher S and Burggren W. Role of hypoxia in the development and evolution of the cardiovascular system, Anti-oxidants and Redox Signaling, 2007,9:152-159. PMID: 17627471
Zhang HY and Fisher SA. Conditioning effect of blood flow on resistance artery smooth muscle myosin phosphatase, Circ Res, 2007,100:730-737. PMID: 17293476.
Sugishita Y, Leifer D, Agani F, Watanabe M, Fisher S. Hypoxia-responsive signaling regulates the apoptosis-dependent remodeling of the embryonic avian cardiac outflow tract, Dev Biol, 2004, 273:285-296. PMID: 15328013
Khatri JK, Joyce KM, Brozovich FV and Fisher S. Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation. J of Biol Chem 276, 37250-37257, 2001. PMID: 11486008.
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