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Vishvanath M. Nene

Vishvanath M. Nene Ph.D.

Academic Title: Adjunct Professor
Primary Appointment: Microbiology and Immunology
Location: HSF-II S-447
Phone: (410) 706-3860
Fax: (410) 706-1482

Personal History:

BSc Physiology & Biochemistry, University of Southampton, UK

PhD Bacterial Genetics, University of Nottingham, UK

I have worked in the field of molecular parasitology for more than 20 years. A considerable portion of my experience is derived from working at The International Livestock Research Institute (ILRI) in Nairobi, Kenya, where I studied Theileria parva, a tick-transmitted intracellular protozoan pathogen which causes a fatal acute lymphoproliferative disease in cattle called East Coast fever (ECF).

Research Interests:

  • Molecular biology and genomics of apicomplexan parasites.
  • Subunit vaccine development & humoral and T-cell immunity.
  • Vector biology and vector-pathogen interactions.

Lab Techniques and Equipment:

  • Molecular biology
  • Genomics


Kaba, S. A., Schaap, D., Roode, E. C., Nene, V., Musoke, A. J., Vlak, J. M. and van Oers, M. M. Improved immnogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens. Vet. Parasitol. 121: 53-64, 2004.

Nene, V., Lee, D., Kang'a, S., Skilton, R., Shah, T., de Villiers, E., Mwaura, Taylor, D., Quackenbush, J. and Bishop, R. Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus infected with Theileria parva. Insect Biochem Mol. Biol. 34:1117-1128, 2004.

Bishop, R., Musoke, A. Morzaria, S., Gardner, M and Nene, V. Theileria: Intracellular protozoan parasite of wild and domestic ruminants transmitted by ixodid ticks. Parasitol. 129: S271, 2004.

Musoke, A., Rowlands, J., Nene, V., Nyanjui, J., Katende, J., Spooner, P., Mwaura, S., Odongo, D., Nkonge, C., Mbogo, S., Bishop, R. and Morzaria, S. Subunit vaccine based on p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenges. Vaccine 23: 3084-3095, 2005.

Guerrero, F.D., Miller, R.J., Rousseau, M-F., Sunkara, S., Quackenbush, J., Lee, Y. and Nene, V. BmiGI: a database of cDNAs expressed in Boophilus microplus, the tropical/southern cattle tick. Insect Biochem. Mol. Biol. 35: 585-595, 2005.

Desjardin, C., Eisen, J. A. and Nene, V. New evolutionary frontiers from unusual virus genomes. Genome Biology 6: 212, 2005.

Kaba, S. A., Musoke, A. J., Schapp, D., Schetters, T., Rowlands, J., Vererulen, A. N., Nene, V., Vlak, J. M., and van Oers, M. M. Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle. Vaccine 23: 2791-2800, 2005.

Pain, A., Renauld, H., Berriman, M., Murphy, L., Yeats, C. A., Weir, W., Kerhornou, A., Aslett, M., Bishop, R., Bouchier, C., Cochet, M., Coulson, R. M. R., Cronin, A., de Villiers, E., Fraser, A., Fosker, N., Gardner, M., Goble, A., Griffiths-Jones, S., Harris, D. E., Katzer, F., Larke, N., Lord, A., Maser, P., McKellar, S., Mooney, P., Morton, F., Nene, V., O'Neil, S., Price, C., Quail, M. A., Rabbinowitsch, E., Rawlings, N. D., Rutter, S., Saunders, D., Seeger, K., Shah, T., Squares, R., Squares, S., Tivey, A., Walker, A. R., Woodward, J., Dobbelaere, D. A. E., Langsley, G., Rajandream, M-A., McKeever, D., Shiels, B., Tait, A., Barrell, R. and Hall, N. Genome of the host-cell transforming parasite Theileria annulata compared with T. parva. Science. 309: 131-133, 2005.

Gardner, M J., Bishop, R., Shah, T., de Villiers, E., Carlton, J. M., Hall, N., Ren, Q., Paulsen, I. T., Pain, A., Berriman, M., Wilson, R. J. M., Sato, S., Ralph, S. A., Mann, D. J., Xiong, Z., Shallom, S. J., Weidman, J., Jiang, L., Lynn, J.,Weaver, B., Shoaibi, A., Domingo, A. R., Wasawo, D., Crabtree, J., Wortman, J. R., Haas, B., Angiuoli, S. V., Creasy, T. H., Lu, C., Suh, B., Silva, J., Utterback, T.R., Feldblyum, T.V., Pertea, M., Allen, J., Nierman, W. C., Taracha, E., Salzberg, S. L., White, O. R., Fitzhugh, H. A., Morzaria, S., Venter, J.C., Fraser, C. M., and Nene, V. Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes. Science. 309: 134-137, 2005.

Lambson, B., Nene, V., Obura, M., Shah, T., Pandit, P., ole-MoiYoi, O., Delroux, K., Welburn, S., Skilton, R., de Villiers, E. and Bishop R. Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells. Insect Mol. Biol. 14: 403-414, 2005.

Bishop, R., Shah, T., Peppe, R., Hoyle, D., Pearson, T., Haines, L., Brass, A., Hulme, H., Graham, S. P., Taracha, E. L. N., Kanga, S., Lu, C., Hass, B., Wortmann, J., White, O., Gardner, M. J., Nene, V. and de Villiers, E. Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage. Nucleic Acids Res. 33: 5503-5511, 2005.

Graham, S. P., Pella, R., Honda, Y., Mwangi, D. M., Tonukari, N. J., Yamage, M., Glew, E. J., de Villiers, E. P., Shah, T., Bishop, R., Abuya, E., Awino, E., Gachanja, J., Luyai, A. E., Mbwika, F., Muthiani, A. M., Ndegwa, D. M., Njahira, M., Nyanjui, J. K., Onono, F. O., Osaso, J., Saya, R. M., Wildmann, C., Fraser, C. M., Maudlin, I., Gardner, M. J., Morzaria, S. P., Loosmore, S., Gilbert, S. C., Audonnet, J-C., van der Bruggen, P., Nene, V. and Taracha, E. L. N. Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes. Proc. Natl. Acad. Sci. 103: 3286-3291, 2006.

Guerrero, F. D., Nene, V., George, J. E., Barker, S. C. and Willadsen, P. Sequencing a new target genome: The southern cattle tick, Boophilus microplus (Acari: Ixodidae) genome project. J. Med. Entomol. 43: 9-16, 2006.

Shah, T., de Villiers, E., Nene, V., Hass, B., Taracha, E., Gardner, M. J., Sansom, C., Pelle, R. and Bishop, R. Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS). Gene. 366: 104-108, 2006.

Guerrero, F.D., Bendele, K.G., Chen, A.C., Li, A.Y., Miller, R.J., Pleasance, E., Varhol, R., Rousseau, M-E. and Nene, V.M. Serial analysis of gene expression in the southern cattle tick following acaricide treatment of larvae from organophosphate resistant and susceptible strains. Insect Mol. Biol. 16: 49-60, 2007.

Graham, S. P, Saya, R., Awino, E., Ngugi, D., Nyanjui, J.K. Kecker, R., Taracha, E. L. N. and Nene, V. Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva. Vet. Immunol. Immunopath. 115: 383-389, 2007.

Graham, S. P., Honda, Y., Pelle, R., Mwangi, D. M., Glew, J., de Villiers, E. P., Shah, T., Bishop, R., van der Bruggen, P., Nene, V., Taracha, E. L. N. A novel strategy for the identification of cytotoxic T lymphocyte target antigens from genomic data. Immunome Res. 3: 2, 2007.

Danley, P. D., Mullen, S. P., Lui, F., Nene, V., Quackenbush, J. and Shaw, K. L. A cricket gene index: a neurological, behavioral and evolutionary genetic resource. BMC Genomics 8: 109, 2007.

Nene, V., Wortman, J. R., Lawson, L., Haas, B., Kodira, C., Tu, Z., Loftus, B., Xi, Z., Megy, K., Grabherr, M., Ren, Q., Zdobnov, E. M., Lobo, N. F., Campbell, K. S., Brown, S. E., Bonaldo, M. F., Zhu, J., Sinkins, S. P., Hogenkamp, D. G., Amedo, P., Arensburger, P., Atkinson, P. W., Bidwell, S., Biedler, J., Birney, E., Bruggner, R. V., Costas, J., Coy, M R., Crabtree, J., Crawford, M., deBruyn, B., DeCaprio, D., Eiglmeier, K., Eisenstadt, E., El-Dorry, H., Gelbart, W. M., Gomes, S. L., Hammond, M., Hannick, L. I., Hogan, J. R., Holmes, M. H., Jaffe, D., Johnston, S. J., Kennedy, R. C., Koo, H., Kravitz, S., Kriventseva, E. V., Kulp, D., LaButti, K., Lee, E., Li, S., Lovin, D. D., Mao, C., Mauceli, E., Menck, C. F. M., Miller, J. R., Montgomery, P., Mori, A., Nascimento, A. L., Naveira, H. F., Nusbaum, C., O'Leary, S. B., Orvis, J., Pertea, M., Quesneville, H., Reidenbach, K. R., Rogers, Y-H., Roth, C. W., Schneider, J. R., Schatz, M., Shumway, M., Stanke, M., Stinson, E. O., Tubio, J. M. C., VanZee, J. P., Verjovski-Almeida, S., Werner, D., White, O., Wyder, S., Zeng, Q., Zhao, Q., Zhao, Y., Hill, C. A., Raikhel, A. S., Soares, M. B., Knudson, D. L., Lee, N. H., Galagan, J., Salzberg, S. L., Paulsen, I. T., Dimopoulos, G., Collins, F. H., Birren, B., Fraser-Liggett, C. M. and Severson, D. W. , Genome sequence of Aedes aegypti, a major arbovirus vector. Science, May 17 2007 (10.1126/science.1138878).

Desjardins, C., Gundersen-Rindal, D., Hostetler, J.,B., Tallon, L. J., Fuester, R. W., Schatz, M., Pedroni, M. J., Fadrosh, D. W., Haas, B. J., Toms, B. S., Chen, D and Nene, V. Structure and evolution of a proviral locus of Glyptapanteles indiensis bracovirus. BMC Microbiol. 7: 61, 2007.

Van Zee, J. P., N.S. Geraci, N. S., Guerrero, F. D., Wikel, S. K., Stuart, J. J., Nene, V. M. and Hill, C. A. Tick genomics: The Ixodes genome project and beyond. Int. J. Parasitol. 37, 1297-1305 (2007).

Jongejan, F., Nene, V., de la Fuente, J., Pain, A. And Willadsen, P. Advances in the genomics in ticks and tick-borne pathogens. Trends Parasitol. 23, 391-396, 2007.