1989: B. S. in Bioengineering, Nankai University, China.
1993: M. S. in Molecular Biology, Nankai University, Tianjin, China
1998: Ph.D. in Immunology, Institute of Virology, Chinese Academy of Preventive Medicine, China.
1998 – 2001: Postdoctoral Fellow, McGill University AIDS Center, Montreal, Canada. (Dr. Mark A. Wainberg’s Lab)
2001 - 2004: Post-Doctoral Research Associate, University of Toronto, ON, Canada. (Dr. Kelly MacDonald’s Lab)
1989 - 1991: Assistant Engineer, Fermentation Research Center, Lian Yuan Gang Yuan Sheng Biochem Inc., Jiangsu, China
1994 – 1998: Research Associate, Lab. of Tumor Viruses and AIDS, Institute of Virology, Chinese Academy of Preventive Medicine, China.
2001 - 2004: Post-Doctoral Research Associate, Dept. of Microbiology, Mount Sinai Hospital/University of Toronto, ON, Canada.
2004 – 2007: Faculty Research Associate, Institute of Human Virology, UMBI, Baltimore, MD, USA
2007 - 2009: Faculty Research Associate, Institute of Human Virology, Department of Microbiology and Immunology, University of Maryland School of Medicine.
2009 - present: Assistant Professor, Institute of Human Virology, Department of Microbiology and Immunology, University of Maryland School of Medicine.
Toward the goal of my research, a protective HIV-1 vaccine, I have been doing AIDS vaccine related studies: from molecular epidemiology studies on HIV-1, design and construction of candidate AIDS vaccines, evaluation of AIDS vaccine concepts in SIV/monkey models, to basic HIV-1 specific T cell and B cell immunity, etc. My expertise in Immunology, Virology and Molecular Biology will continuously make contributions on AIDS vaccine related scientific studies.
My work in IHV started from characterization of the proliferation and differentiation of antigen specific human CD4 T cells during in vitro primary immune responses. We demonstrated that human CD4 T cells could self-defend against R5 HIV-1 by secreting anti-viral chemokines during primary immune responses. Since HIV-1 can preferentially infect HIV-1 specific CD4 T cells in vivo, our observation pointed out that AIDS vaccine candidate that could selectively elicit CD4 T cell responses capable of ‘‘self-protection’’ from HIV-1 infection is a potential new strategy in the quest for an AIDS vaccine.
Currently, my works focus on the identification of protective Ab response from HIV-1 controller. We demonstrated discordance between Env specific memory B cells and serologic anti-Env antibodies in NVS and indicated the importance of memory B cell pool as a historical reservoir of humoral immune responses. A novel method for rapid cloning of human mAbs from memory B cell was developed to isolate potential protective mAbs. 50 novel mAbs against gp120 have been identified. Some of them showed potent neutralizing activity and/or ADCC function. Mapping their epitopes will help guide the development of AIDS vaccine. In addition, our novel techniques of cloning human mAbs are easy to be adapted for isolating human mAbs against other pathogenic viral antigens, toxins and cancers.
In the meantime, we are developing new vaccine constructs based on native Env trimers.
Patents, Inventions, Copyrights:
Patent pending: A general method for generating human antibody responses in vitro. (Patent Pub. No: WO/2007/134220, International Application No.: PCT/US2007/068752, Publication Date: 22.11.2007, International Filing Date: 11.05.2007)
Patent application: Rapid Expression Cloning of Human Monoclonal Antibodies from Memory B cells. (UMB001-046 GL-2008-088, Apllication#61/114,047)
Zhang J, Guan Y, Zhang Y, et al. Chloroplast gene psbA contain eukaryotic regulatory sequences originated from nuclear genome. SCIENCE IN CHINA (series B), 1994, 24(4): 371-375.
Shao Y, Guan Y, Zeng Yi, et al. Genetic variation and molecular epidemiology of the Ruili HIV-1 strains of Yunnan in 1995. Chinese Journal of Virology, 1996;12(1):9-17
Zhao Q, Guan Y, Zeng Yi, Shao Y, et al. Research on the biological character of the Ruili human immunodeficiency virus strains isolated in 1995. Chinese Journal of Experimental and Clinical Virology, 1996; 10(4): 366-369
Bai X, Guan Y, Zhang Y, Shao Y, et al. Sequencing and genotype analysis of HIV-1 gp120 gene C2-V3 region of Xinjiang strains. Chinese Journal of Virology, 1997, 13(4):339-344.
Chen J, Guan Y, Shao Y, et al. Sequence analysis of HIV-1 C2-V3 region of Longchuan strains. Chinese Journal of Microbiology and Immunology, 1997;17(1):1-6.
Guan Y, Chen J, Zeng Y, Shao Y et al. Subtype and sequence analysis of the C2-V3 region of gp120 genes among HIV infected IDUs in Ruili epidemic area of Yunnan Province, China. Chinese Journal of Experimental and Clinical Virology, 1997; 11(1):8-12.
Guan Y, Zhu Y,Liu H, Zhou L and Zeng Y. Preliminary study on DNA vaccine of Chinese HIV-1 strains. Chinese J Virology. 2000, 16(4): 322-6.
Guan Y, Liu H, Zhu Y, Zhou L, Du B, Zeng Y. Construction and expression of recombinant adeno-associated HIV-1 virus. Chinese Journal of Experimental & Clinical Virology. 2000;14(4):322-4.
Guan Y, Whitney JB, Diallo K, Wainberg MA. Leader sequences downstream of the primer binding site are important for efficient replication of simian immunodeficiency virus. J Virol. 2000 Oct;74(19):8854-60.
Rong L, Russell RS, Hu J, Guan Y, Kleiman L, Liang C, Wainberg MA. Hydrophobic amino acids in the human immunodeficiency virus type 1 p2 and nucleocapsid proteins can contribute to the rescue of deleted viral RNA packaging signals. J Virol. 2001 Aug;75(16):7230-43.
Guan Y, Diallo K, Detorio M, Whitney JB, Liang C, Wainberg MA. Partial restoration of replication of simian immunodeficiency virus by point mutations in either the dimerization initiation site (DIS) or Gag region after deletion mutagenesis within the DIS. J Virol. 2001 Dec;75(23):11920-3.
Guan Y, Diallo K, Whitney JB, Liang C, Wainberg MA. An intact U5-leader stem is important for efficient replication of simian immunodeficiency virus. J Virol. 2001 Dec;75(23):11924-9.
Guan Y, Whitney JB, Liang C, Wainberg MA. Novel, live attenuated simian immunodeficiency virus constructs containing major deletions in leader RNA sequences. J Virol. 2001 Mar;75(6):2776-85.
Guan Y, Whitney JB, Detorio M, Wainberg MA. Construction and in vitro properties of a series of attenuated simian immunodeficiency viruses with all accessory genes deleted. J Virol. 2001 May;75(9):4056-67.
Whitney JB, Oliveira M, Detorio M, Guan Y, Wainberg MA. The M184V mutation in reverse transcriptase can delay reversion of attenuated variants of simian Immunodeficiency virus. J Virol, 2002 Sept; 76(17):8958-62.
Li H, Guan Y, Szczepanska A, Moreno-Vargas AJ, Carmon AT, Robin I, Lewis GK and Wang LX. Synthesis and anti-HIV activity of trivalent CD4-mimetic miniproteins. Bioorganic & Medicinal Chemistry. 2007, 15(12): 4220-4228.
Guan Y, Abdelwahab S, Kamin-Lewis R, DeVico AL, and Lewis GK. ‘Self-protection’ of individual CD4 T cells against R5 HIV-1 infection by the synthesis of anti-viral CCR5 ligands. PLoS One, 2008, 3(10): e3481.
Guan Y, Sajadi MM, Kamin-Lewis R, Fouts T, Dimitrov A, Zhang Z, Redfield R, DeVico AL, Gallo R and Lewis GK. Discordant memory B cell and circulationg anti-Env antibody responses in HIV-1 infection. Proc Natl Acad Sci U S A. 2009 106(10):3952-3957(epub.Feb18,2009).
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