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Alessio Fasano
M.D.
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| Academic Title:
Professor |
| Primary Appointment:
Pediatrics |
| Secondary Appointments:
Medicine, Physiology |
| Additional Title(s):
Director, Center for Celiac Research; Director, Mucosal Biology Research Center |
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afasano@mbrc.umaryland.edu |
| Location:
HSFII
S345 |
| Phone:
(410) 706-5501 |
| Fax:
(410) 706-5508 |
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Personal History
EDUCATION
1975-81: School of Medicine, University of Naples (Italy); Doctor of Medicine
1981-84: Pediatric resident, 2nd Clinic, 2nd School of Medicine, University of Naples, (Italy).
1981-85: School of Medicine, University of Naples (Italy); Board-certified Pediatrician
2003-04: Johns Hopkins University School of Medicine, Hopkins Business of Medicine Graduate.
ACADEMIC AWARDS AND DISTINCTIONS
1981: Graduated Summa cum Laude
1985: Pediatric board Summa cum Laude
1991: J. Harries prize for the best presentation at the European Society of Pediatric Gastroenterology and Nutrition (ESPGAN); London (U.K.)
1997: Elected member, American Society for Clinical investigations
1998: Voted among the best 25 Pediatricians in Baltimore
1998: Voted Teacher of the Year by the 2nd year Medical School students at UMB
2002: Voted Teacher of the Year for the Academic Year 2001-2002
2003: Elected Alpha Omega Alpha Medical Honor Society
2003: Voted Teacher of the Year for the Academic Year 2002-2003
2004: Physician Hero Award, Health Care Hero Awards
2005: Student Council Faculty Award
2005: Maryland Innovator of the Year Award
2005: Finalist, National Institutes of Health, Director’s Pioneer Award
2006: "Best Academic/Industry Collaboration", Greater Baltimore Committee, Bioscience Award
2006: Student Council Faculty Award
2006: Entrepreneur of the Year Award
Research Interests
Dr. Fasano’s research focuses on mucosal biology, crossing the disciplines of microbiology, molecular and cell biology, and physiology. By focusing on the cross talk between enteric pathogens and their hosts, Dr. Fasano’s group have elucidate various intracellular signaling pathways that govern eukaryotic cell functions. Among others, Dr. Fasano discovered and characterized several new bacterial toxins involved in the pathogenesis of diarrheal diseases. This information has been used to develop new attenuated vaccines, including cholera and shigella. The characterization of one of these toxins (Zonula Occludens Toxin from Vibrio cholerae) led to the discovery of a molecule, named zonulin, involved in the regulation of the permeability of intercellular tight junctions of epithelial and endothelial barriers. This discovery shed light on the regulation of molecules trafficking between body compartments and mechanisms of innate immunity. Dr. Fasano’s research has linked zonulin to the pathogenesis of a series of autoimmune diseases, including type 1-diabetes, celiac disease, and multiple sclerosis. This information was used to create a platform research plan with ramifications in embryogenesis, drug, antigen, and gene delivery, immunomodulation, and prevention and treatment of inflammatory, autoimmune, and neoplastic diseases.
Publications
R. Lu, W. Wang, S. Uzzau, R. Vigorito, H.R. Zielke, A. Fasano. Affinity Purification and Partial Characterization of the Zonulin/Zonula Occludens Toxin (Zot) Receptor from Human Brain. J. Neurochem. 2000, 74:320-326.
I. Hill, A. Fasano, R. Schwartz, D. Counts, M. Glock, K. Horvath. The prevalence of celiac disease in at risk groups of children in United States. Ped. Res. 2000;136(1):86-90.
A. Fasano, T. Not, W. Wang, S. Uzzau, I. Berti, A. Tommasini, S.E. Goldblum. Zonulin, a newly discovered modulator of intestinal permeability, and its expression in coeliac disease. Lancet 2000:358;1518-1519.
D.Sblattero, I. Berti, C. Trevisol, R. Marzari, A. Tommasini, A. Bradbury, A. Fasano, A. Ventura, T. Not. Human tissue transaminase ELISA: a powerful mass screening diagnostic assay for celiac disease. Am J. Gastroenterol. 2000:98:1253-1257..
F. Raimondi, J.P.Y. Kao, C. Fiorentini, A. Fabbri, G. Donelli, N. Gasparini, A. Rubino, A. Fasano. Enterotoxicity and Cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin in vitro systems. Infect. Immun. 2000;68:3180-3185.
S. Uzzau and A. Fasano. Cross talk between enteric pathogens and the intestine. Cell Microbiol. 2000: 2:83-89.
V. H. Shah, H. Rotterdam, D.P. Kotler, A. Fasano, P.H.R. Green. All that Scallops is not celiac disease. Gastrointest. Endosc. 2000:51:717-720.
W. Wang, R. Lu, M.R. Di Pierro, A. Fasano. Zonula Occludin Toxin, a Microtubule Binding Protein. World J. Gastroenterol 2000;6:330-334.
V. Baldas, A. Tommasini, C. Trevisiol, I. Berti, A. Fasano, D. Sblattero, A. Bradbury, R. Marzari, A. Ventura, T. Not. The development of a novel rapid non-invasive screening test for celiac disease. Gut 2000;47:628-631.
A McVeigh, A. Fasano, D A Scott, D C Robertson, S J Savarino. Molecular Characterization of an Allelic Variant of Enteroaggregative Escherichia coli Heat-Stable Enterotoxin from Enterotoxigenic Escherichia Coli. Infect. Immun. 2000:68:5710-5715.
W. Wang, S. Uzzau, S.E. Goldblum, A. Fasano. Human Zonulin, a Potential Modulator of Intestinal Tight Junctions. J. Cell Sci. 2000; 113:4435-4440.
S. Uzzau, R. Lu, W. Wang, C. Fiore, A. Fasano Purification and Preliminary Characterization of the Zonula Occludens Toxin Receptor from Human (CaCo2) and Murine (IEC6) intestinal Cell Lines. FEMS Microbiol Lett. 2001 Jan 1;194(1):1 5.
A. Fasano. Bacterial Infections: small intestine and colon. Cur. Opin. Gastroenterol 2001; 17:4-9.
A. Fasano. Regulation of intercellular tight junctions by Zonula occludens toxin (Zot) and its eukaryotic analogue zonulin. Ann. New York Acad. Sci. 2001; 915:214-222.
A. Fasano. The genome of the smartest pathogen decoded: is the cholera war over? J Pediat Gastroenterol Nutr 2001; 32:1-3.
A. Fasano, C. Catassi. Current Approaches to Diagnosis and Treatment of Celiac Disease: An Evolving Spectrum. Gastroenterology 2001;120:636 651.
A. Fasano. Celiac disease: the past, the present, the future Pediatrics 2001; 107:768-770.
T.L. Watts, A. Fasano. Modulation of Intestinal Permeability: a Novel and Innovative Approach for the Oral Delivery of Drugs, Macromolecules, and Antigens. Biothec Gen Engin Rev 2001; 16:433-453.
A. Fasano. Vibrio cholerae genome decoded: is the war over? J. Pediatr Gastroenterol Nutr 2001; 32:1-3
F. Raimondi, J. B. Kaper, E. C. Boedecker, Marcia K. Wolf, S. Guandalini, A. Fasano. Enteropathogenic E. Coli strain RDEC-1 produces a novel enterotoxic factor active in rabbit ileum in vitro. J. Pediatr Gastroenterol Nutr 2001; 32:122-126
M. Di Pierro, A. Fasano. Zonula occludens toxin: an innovative method of oral drugs delivery. Minerva Pediatr. 2001;53:15 22..
M. Di Pierro, R. Lu, S.Uzzau, W.Wang, K. Margaretten, C. Pazzani, F. Maimone, and A. Fasano. Zonula occludens toxin structure –function analysis: identification of the fragment biologically active on tight junctions and of the zonulin receptor binding domain. J Biol Chem 2001; 276:19160 19165.
A. Rabsztyn, P.H.R. Green, I. Berti, A. Fasano, J.A. Perman, K. Horvath. Macroamylasemia in patients with active celiac disease. Am J Gastroenterol 2001;96:1096-1100.
A. Fasano. Nitric oxide and intestinal water and electrolyte transport: in, out, or both? J. Pediatr Gastroenterol Nutr 2001;32:527 8.
A. Fasano. Intestinal zonulin: open Sesame! Gut 2001;49:159 62.
E. Bucci, T.L. Watts, H.E. Kwansa A. Fasano, B.A. Matheson, A. Rebel, R.C. Koehler. Cell free Hemoglobin, Oxygen Off Load and Vasoconstriction. Anasthesiol Intensivmed Notfallmed Schmerzther 2001;36:S123 4.
Fabiani E, Catassi C and the International Working Group on Eu-tTG (*includes A. Fasano). The serum IgA class anti-tissue transglutaminase antibodies in the diagnosis and follow-up of coeliac disease. Results of an international multi-centre study. Eur J Gastroenterol Hepatol 2001;13: 659-665.
M.M. Pietzak, C. Catassi, S. Drago, F. Fornaroli, and A. Fasano. Celiac Disease: Going Against the Grains. Nutr Clin prac 2001;16:335-344.
S. Drago, M Di Pierro, C. Catassi, A. Fasano. Recent developments in the pathogenesis, diagnosis and treatment of celiac disease. Expert Opin Ther Patents 2002;12:45-51.
M. Rossi, F. Maurano, D. Luongo, A. Fasano, S. Uzzau, S. Auricchio, R. Troncone. Zonula occludens toxin (Zot) interferes with the induction of nasal tolerance to gliadin. Immunol Let, 2002;1:217 221.
C Catassi, A Fasano. New developments in childhood celiac disease. Curr Gastroenterol Rep 2002;4:238 243.
A. Fasano. Toxins and the gut: role in human diseases. Gut, 50 Suppl 3:III9 III14, 2002.
A. Alaedini, PH Green, HW Sander, AP Hays, ET Gamboa, A Fasano, M Sonnenberg, LD Lewis, N Latov. Ganglioside reactive antibodies in the neuropathy associated with celiac disease. J Neuroimmunol 2002, 127: 145-148.
RM Vigneulle, S. Rao, A. Fasano, TJ MacVittie. Structural and functional alterations of the gastrointestinal tract following radiation induced injury in the rhesus monkey. Dig Dis Sci 2002;47:1480-91.
G. Davidson, G. Barnes, D. Bass, M. Cohen, A. Fasano, O. Fontaine, S. Guandalini. Infectious diarrhea in children: working group report of the first world congress of pediatric gastroenterology, hepatology, and nutrition. J Ped Gastroenterol Nutr 2002; 35 (Suppl 2):S143 50.
R. El Asmar, P. Panigrahi, P. Bamford, I. Berti,T. Not, G.V. Coppa, C. Catassi, A. Fasano. Host-Dependent Activation of the Zonulin System is Involved in the Impairment of the Gut Barrier Function Following Bacterial Colonization. Gastroenterology 2002;123:1607-1615.
C. Catassi, F. Fornaroli, A. Fasano. Celiac disease: From basic immunology to bedside practice. Clin Appl Immunol Rev 2002;.3:61-71.
E. Bucci, T.L. Watts, H.E. Kwansa, A. Fasano. Different efficacy in vitro of hemoglobin based oxygen carriers and red cells. Adv Exp Med Biol 2003;510:89-92.
M.G. Clemente, S. De Virgiliis, J. S. Kang, R. Macatagney, M.P. Musu, M.R. DiPierro, S. Drago, M. Congia, A. Fasano. Early effects of gliadin on enterocyte intracellular signalling invovled in intestinal barrier function. Gut 2003;52:218-223.
A. Fasano. European and North American populations should be screened for coeliac disease. Gut 2003;52:168-169.
N.N. Salama, A. Fasano, R. Lu, N.D. Eddington. Effect of the biologically active fragment of zonula occludens toxin, DeltaG, on the intestinal paracellular transport and oral absorption of mannitol. Int J Pharm 2003;251:113 121.
C.S. Karyekar, A. Fasano, S. Raje, R. Lu, T.C. Dowling, N.D. Eddington. Zonula occludens toxin increases the permeability of molecular weight markers and
chemotherapeutic agents across the bovine brain microvessel endothelial cells. J
Pharm Sci 2003;92:414 423.
A. Fasano, I. Berti, T. Gerarduzzi, T. Not, R.B. Colletti, S. Drago, Y. Elitsur, P.H.R. Green, S. Guandalini, I. Hill, M. Pietzak, A. Ventura, M. Thorpe, D. Kryszak, F. Fornaroli, S.S. Wasserman, J.A. Murray, K. Horvath. Prevalence of celiac disease in at-risk and not-at-risk groups in the United States: a large multicenter study. Arch Int Med 2003;163:286-292.
M. Marinaro, A. Fasano, M.T. De Magistris. Zonula Occludens Toxin acts as an adjuvant through different mucosal routes and induces protective immune responses. Infect. Immun. 2003;71:1897-1902.
A. Fasano. Bioterrorism and Biological Warfare: not only a respiratory affair. J. Ped. Gastroenterol Nutr. 2003;36:305-306.
Fornaroli F, Drago S, Di Pierro MR, Catassi C, Fasano A. Celiac disease. A world in exploration. Minerva Pediatr 2003;55:23-31.
A. Fasano. Celiac Disease – How to Handle a Clinical Chameleon. N Engl J Med 2003: 348;2568-70.
Neri E, Not T, Horvath K, Kryszak D, Drago S, Di Pierro MR, Tommasini A, Ventura A, Fasano A. Human tissue transglutaminase ELISA and an old study: a revision of the blood donor screening study for coeliac disease in the USA. Scand J Gastroenterol. 2004;2:195-7.
N. N. Salama, A. Fasano, M. Thakar, and N. D. Eddington. The Effect of ¿G On the Transport and Oral Absorption of Macromolecules. J. Pharm Sci. 2004;93:1310-9.
A. Fasano, J.P. Nataro. Intestinal epithelial tight junctions as targets for enteric bacteria-derived toxins. Adv Drug Deliv Rev. 2004;56:795-807.
E. Salazar-Lindo, S. Allen, D.R. Brewster, E.J. Elliott, A. Fasano, A.I. Phillips, I.R. Sanderson, P.I. Tarr, Latin American Society for Pediatric Gastroenterology, Hepatology and Nutrition. Intestinal infections and environmental enteropathy: working group report of the second world congress of pediatric gastroenterology, hepatology, and nutrition. J Pediatr Gastroenterol Nutr. 2004:39:S662-9.
T.C. Johnson, B. Diamond, L. Memeo, H. Negulescu, Z. Hovhanissyan, V. Verkarre, H. Rotterdam, A. Fasano, S. Caillat-Zucman, E. Grosdidier, R. Winchester, C. Cellier, B. Jabri, P.H. Green. Relationship of HLA-DQ8 and severity of celiac disease: comparison of New York and Parisian cohorts. Clin Gastroenterol Hepatol;2004;2:888-94.
M.G. Clemente, M.P. Musu, R. Troncone, U. Volta, M. Congia, C. Ciacci, E. Neri, T. Not, G. Maggiore, P. Strisciuglio, G.R. Corazza, G. Gasbarrini, L. Cicotto, G. Sole, A. Fasano, S. De Virgiliis. Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study. Am J of Gastroenterol 2004;99:1551-6.
C. Catassi and A. Fasano. Celiac disease as a cause of growth retardation in childhood. Curr Opin Peds 2004;16:445-9.
A. Fasano and J.P. Nataro. Intestinal epithelial tight junctions as targets for enteric bacteria-derived toxins. Adv Drug Deliv Rev. 2004;56:795-807.
F. Katouzian, D. Sblattero, T. Not, A. Tommasini, E. Giusto, D. Meiacco, M. Stebel, R. Marzari, A. Fasano,A. Ventura. Dual sugar gut-permeability testing on blood drop in animal models. Clin Chim Acta. 2005;352:191-7.
I.D. Hill, M.H. Dirks, G.S. Liptak, R.B. Colletti, A. Fasano, S. Guandalini, E.J. Hoffenberg, K. Horvath, J.A. Murray, M. Pivor, E.G. Seidman. Guideline for the Diagnosis and Treatment of Celiac Disease in Children: Recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition. J Pediatr Gastroenterol Nutr. 2005;40:1-19.
T. Watts, I. Berti, A. Sapone, T. Gerarduzzi, T. Not, R. Zielke, A. Fasano. Role of the intestinal tight junction modulator zonulin in the pathogenesis of type I diabetes in BB diabetic prone rats. Proc Natl Acad Sci USA 2005;102:2916-21.
A. Fasano. Clinical presentation of celiac disease in the pediatric population. Gastroenterology 2005;128(4 Suppl 1):S68-73.
C.E. Semrad, A. Fasano, A. Picarelli, L. Sabbatella, K. Sullivan. Seeing the light with celiac. Clin Immunol. 2005;115:236-9.
A. Fasano and C. Catassi. Coeliac disease in children. Best Pract. Res. Clin. Gastroenterol. 2005;19:467-78.
E. Smecuol, E. Sugai, S. Niveloni, H. Vazquez, S. Pedreira, R. Mazure, M.L. Moreno, M. Label, E. Maurino, A. Fasano, J. Meddings, J.C. Bai. Permeability, zonulin production, and enteropathy in dermatitis herpetiformis. Clin. Gastroenterol. Hepatol. 2005;3:335-41.
A. Fasano and T. Shea-Donohue. Mechanisms of Disease: the role of intestinal barrier function in the pathogenesis of gastrointestinal autoimmune diseases. Nat. Clin. Prac. Gastro. & Hepatol. 2005;2:416-22.
C.R. Taddei, A. Fasano, A.J. Ferreira, L.R. Trabulsi M.B. Martinez. Secreted autotransporter toxin produced by a diffusely adhering Escherichia coli strain causes intestinal damage in animal model assays. FEMS Microbiol Lett. 2005;250:263-9.
S. Gadewar. A. Fasano. Current concepts in the evaluation, diagnosis and management of acute infectious diarrhea. Curr Opin Pharmacol. 2005;5:559-65.
D. Menon, C.S. Karyekar A. Fasano, R. Lu, N.D. Eddington. Enhancement of brain distribution of anticancer agents using DeltaG, the 12kDa active fragment of ZOT. Int. J Pharm. 2005;306:122-31.
S. Gadewar, A. Fasano. Celiac disease: is the atypical really typical? Summary of the recent national institutes of health consensus conference and latest advances. Curr Gastroenterol Rep. 2005;7:455-61.
M.D. Angelis, C.G. Rizzello, A. Fasano, M.G. Clemente, C.D. De Simone, M. Silano, M.D. Vincenzi, I Losito, M Gobbetti. VSL#3 probiotic preparation has the capacity to hydrolyze gliadin polypeptides responsible for Celiac Sprue probiotics and gluten intolerance. Biochim Biophys Acta. 2006;1762:80-93.
A.E. Kalaydjian, W. Eaton, N Cascella, A. Fasano. The gluten connection: The association between schizophrenia and celiac disease. Acta Psychiatr. Scand 2006;113:82-90.
S. Drago, R. El Asmar, M. DiPierro, A. Tripathi, M. Clemente, A. Sapone, M. Thakar, G. Iacono, A. Carroccio, C. D’Agate, T. Not, L. Zampini, C. Catassi, and A. Fasano. Gliadin, Zonulin, And Gut Permeability: Effects on Celiac and Non-Celiac Intestinal Mucosa and Intestinal Cell Lines. Scan. J Gastroenterol. 2006;41:408-419.
K. E. Thomas, A. Sapone, A. Fasano, and S.N. Vogel. Gliadin Stimulation of Murine Macrophage Inflammatory Gene Expression and Intestinal Permeability Are MyD88-Dependent: Role of the Innate Immune Response in Celiac Disease. J Immunol 2006;176:2512-2521.\
Fasano A, Ghandehari H. Challenges in pediatric drug delivery: the case of vaccines. Adv Drug Deliv Rev. 2006; 58:1-3.
Salama NN, Eddington ND, Fasano A. Tight junction modulation and its relationship to drug delivery. Adv Drug Deliv Rev. 2006;58:15-28.
Gelfond D, Fasano A. Celiac disease in the pediatric population. Pediatr Ann 2006;35:275-79.
Motlekar NA, Fasano A, Wachtel MS, Youan BB. Zonula occludens toxin synthetic peptide derivative AT1002 enhances in vitro and in vivo intestinal absorption of low molecular weight heparin. J Drug Target. 2006;14:321-9.
A. Sapone, L. de Magistris, M. Pietzak, M.G. Clemente, A. Tripathi, F. Cucca, R. Lampis, D. Kryszak, M. Carteni, M. Generoso, D. Iafusco, F. Prisco, F. Laghi, G. Riegler, R. Carratu, D. Counts and A. Fasano. Zonulin up-regulation is associated to increase in gut permeability in subjects with Type 1 diabetes and their relatives. Diabetes 2006;55:1443-9.
D. Branski, A. Fasano, R. Troncone. Latest developments in the pathogenesis and treatment of celiac disease. J Pediatr. 2006;149:295-300.
Fasano A. Systemic autoimmune disorders in celiac disease. Curr Opin Gastroenterol. 2006;22:674-9.
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