SOM Faculty Profile : Michael S Donnenberg

Michael S Donnenberg

Michael S Donnenberg M.D.

Academic Title: Professor

Primary Appointment: Medicine

Secondary Appointments: Microbiology and Immunology

MDONNENB@umaryland.edu

Location: HSF2 S403D

Phone: (410) 706-7560

Fax: (410) 706-8700

Research Interests

Our laboratory is primarily interested in interactions between pathogenic Escherichia coli and host cells. We use combined molecular biology, cell biology and biochemical approaches to gain insight into the processes by which pathogenic E. coli cause disease. The goal is to identify and characterize bacterial factors that are involved in interactions with host cells and to identify cellular mechanisms triggered by interactions with bacteria. Our initial approach, to identify, clone and mutate bacterial genes involved in pathogenesis has led to the characterization of large gene clusters and pathogenicity islands required for adherence, host cell damage, and avoidance of host immune responses. This has led to our current efforts to delineate the functions and cellular targets of the protein products of these genes. It is anticipated that these studies will impact not only our understanding and approach to infectious diseases, but also our appreciation for normal and abnormal cellular physiology.

Publications

Klapproth JMA, Scaletsky ICA, McNamara BP, Lai L-C, Malstrom C, James SP, Donnenberg MS. A Large Toxin from Pathogenic Escherichia coli Strains that Inhibits Lymphocyte Activation. Infect Immun, 2000; 68:2148-2155.

Anantha RP, Stone KD, Donnenberg MS. The Effects of bfp Mutations on the Biogenesis of Functional Enteropathogenic Escherichia coli Type IV Pili. J Bacteriol, 2000; 182:2498-2506.

Tacket CO, Sztein MB, Losonsky G, Abe A, Finlay BB, McNamara BP, Fantry G, James SP, Nataro JP, Levine MM, Donnenberg MS. The Role of EspB in Human Enteropathogenic Escherichia coli (EPEC) Infection. Infect Immun, 2000; 68:3689-3695.

Blank TE, Zhong H, Bell AL, Whittam TS, Donnenberg MS. Molecular Variation among Type IV Pilin (bfpA) Genes from Diverse Enteropathogenic Escherichia coli Strains. Infect Immun, 2000; 68:7028-7038.

McNamara BP, Koutsouris A, O’Connell CB, Nougayréde J-P, Donnenberg MS*, Hecht G*. Translocated EspF Protein from Enteropathogenic Escherichia coli (EPEC) Disrupts Host Intestinal Barrier Function. J Clin Invest, 2001; 107:621-629. *contributed equally.

Crane JK, McNamara BP, Donnenberg MS. Role of EspF in Host Cell Death Induced by Enteropathogenic Escherichia coli. Cell Microbiol, 2001; 3:197-211.

Bahrani-Mougeot FK, Buckles EL, Lockatell CV, Hebel JR, Johnson DE, Tang CM, Donnenberg MS. Type-1 fimbriae and Extracellular Polysaccharides are Preeminent Uropathogenic Escherichia coli Virulence Determinants in the Murine Urinary Tract. Mol Microbiol, 2002; 45:1079-1093. PM: 12180926

Welch RA, Burland V, Plunkett III G, Redford P, Roesch P, Rasko D, Buckles EL, Liou S-R, Boutin A, Hackett J, Stroud D, Mayhew GF, Rose DJ, Zhou S, Schwartz, DS, Perna NT, Mobley HLT, Donnenberg MS, Blattner FR. Extensive Mosaic Structure Revealed by the Complete Genome Sequence of Uropathogenic Escherichia coli. Proc Natl Acad Sci, USA, 2002; 99:17020-17024. PM: 12471157

Crowther LJ, Anantha RP, Donnenberg MS. The Inner Membrane Subassembly of the Enteropathogenic Escherichia coli Bundle-Forming Pilus Machine. Mol Microbiol, 2004;51:67-79. PM:15049811

O'Connell CB, Creasey EA, Knutton S, Elliott S, Crowther LJ, Luo W, Albert MJ, Kaper JB, Frankel G, Donnenberg MS. SepL, a Protein Required for Enteropathogenic Escherichia coli type III Translocation, Interacts with Secretion Component SepD. Mol Microbiol, 2004; 52:1613-1625. PM: 15186412

Nougayrède JP, Donnenberg MS. Enteropathogenic Escherichia coli EspF is Targeted to Mitochondria and is Required to Initiate the Mitochondrial Death Pathway. Cell Microbiol, 2004; 6: 1097-1111. PM: 15469437

Crowther LJ, Craig L, Tainer JA, Donnenberg MS. The ATPase Activity of BfpD is Greatly Enhanced by Zinc and Allosteric Interaction with Other Bfp Proteins. J Biol Chem, 2005; 280: 24839 – 24848. PM: 15866879

Ramboarina S, Fernandes PJ, Daniell S, Islam S, Simpson P, Frankel G, Booy F, Donnenberg MS, Matthews S. Structure of the Bundle-Forming Pilus from Enteropathogenic Escherichia coli. J.Biol.Chem, 2005; 280:40252-40260. PM: 16172128

Luo W and Donnenberg MS. Analysis of the Function of Enteropathogenic Escherichia coli EspB by Random Mutagenesis. Infect Immun, 2006 74:810-820.

Links of Interest

The Donnenberg Lab

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