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Principles of Fluorescence Spectroscopy, Third Edition, J. R. Lakowicz

The third edition of this established classic text reference, Principles of Fluorescence Spectroscopy, will enhance upon the earlier editions' successes. Organized as a textbook for the student or the researcher needing to acquire the core competencies, Principles of Fluorescence Spectroscopy, Third Edition, will maintain the emphasis on basics, while updating the examples to include recent results from the literature. The third edition also includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes, and radiative decay engineering. This full-color textbook features the following:

  • Problem sets following every chapter
  • Glossaries of commonly used acronyms and mathematical symbols
  • Appendices containing a list of recommended books that expand on various specialized topics
  • Sections describing advanced topics will indicate as such, to allow these sections to be skipped in an introductory course, allowing the text to be used for classes of different levels
  • Includes CD-ROM of all figures in a low-res format, perfect for use in instruction and presentations

Principles of Fluorescence Spectroscopy, Third Edition, is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology, and medicine.

Fluorescence 1 and 2

Previous Editions:  

Plenum Press 1983, 233 Spring Str. New York, NY 10013 

Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. An introduction to the fundamental aspects of fluorescence spectroscopy, this volume fills the need for a basic text and reference source on the subject. The volume discusses all basic phenomena and their various biochemical applications. The presentation is kept as simple as practicable, and wherever extensive equations are used, a good deal of text is included to explain the origin and meaning of each expression.

Each chapter begins with the theoretical basis of each phenomenon of fluorescence, followed by examples that illustrate the use of the phenomenon in the study of biochemical problems. The volume contains a wealth of figures to help the neophyte grasp the concepts more easily. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, protein fluorescence, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included that illustrate the concepts described in each chapter. An additional chapter is devoted to the instrumentation used in fluorescence spectrometry-an especially valuable feature for those performing or contemplating fluorescence measurements. The volumes includes extensive references to the primary literature of fluorescence spectroscopy that will be useful for those who need to go on to more advanced study.

A lucid presentation of a methodology used extensively in biochemical, chemical, and medical research, Principles of Fluorescence Spectroscopy will be of interest to students and professionals in the fields of biophysics, biochemistry, analytical chemistry as well as in applied spectroscopy, molecular biology, and immunobiology.


The Journal of FluorescencePrinciples of Fluorescence

 The Journal of Fluorescence (JoF) captures the new and emerging trends in fluorescence spectroscopy and its applications. Today, the JoF is the major repository for fluorescence-based material in the world. During the last years the JoF has undertaken many changes, mostly in-line with the global changes occurring in electronic peer-reviewed publishing.



Plasmonics is an international forum for the publication of peer-reviewed leading-edge original articles that both advance and reports on free-metal electrons, plasmons. Topics covered include notable advances in the theory, physics, and applications of surface plasmons in metals, to the rapidly emerging areas of nanotechnology, biophotonics, sensing, biochemistry and medicine. Topics include the theory, synthesis and optical properties of noble metal nanostructures, patterned surfaces or materials, continuous or grated surfaces, devices, or wires for their applications.


Journal of Biomedical Optics 

Founding Editor: Joseph R. Lakowicz

Journal of Biomedical Optics publishes papers on the use of optical technology for biomedical research since 1996 and played a role in recent growth in the use of optics technology for biomedical research.   

Topics in Fluorescence Spectroscopy Series

Topics 1-5This book series describes current trends and emerging topics in fluorescence spectroscopy. Most notably, a volume entitled “Radiative Decay Engineering”, which is directly related to current CFS scientific interests. To date 10 books have been published in this edited volume series with contributions from many authors from around the world. The latest volume, Volume 11, “Glucose Sensing”, was recently published.

Previous Editions:  

Plenum Press, 1991-2007, 233 Spring Str. New York, NY 10013
Editor Joseph R. Lakowicz

Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of macromolecules, to probe the structure of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry.

Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial two volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy.

The first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3). Volume 4, reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. Volume 5 describe nonlinear and two-photon induced fluorescence. Additional volumes in this series will be published to reflect further advances in fluorescence spectroscopy and its many applications. I welcome your suggestions for future topics or volumes, offers to contribute chapters on specific topics, or comments on the present volumes.

Finally, I thank all the authors for their excellent contributions, and for their patience with the inevitable delays incurred in release of this volume.

Annual Reviews in Fluorescence

The Reviews in Fluorescence (RIF) serve as comprehensive collections of trends and emerging hot topics in the field of Fluorescence. The volumes summarize the year’s progress with authoritative analytical reviews, specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. The volumes allow detailed lengthy review articles, not practical to be published in journals.

Who’s Who in Fluorescence Annual Volume

The Who’s Who in Fluorescence (WWIF) serves as a comprehensive archive of fluorescence workers interests, contact details and specialty keywords. Entry into the volume is restricted and is dependent on academic achievements, such as publications etc, in the research areas of fluorescence and biomedical sensing. To date 4 paper-back yearly volumes have been published (2003-6). The 2006 volume features some 366 entries, from no fewer than 36 countries.