By Multi-Photon Excitation (MPE) we mean the simultaneous absorption of two or more long wavelength photons followed by fluorescence emission from the lowest excited state.
The goal of this CFS long-term project is to study the basic principles and biochemical applications of MPE.MPE offers a localized excitation in fluorescence microscopy and an enhanced photoselection in spectroscopy.
We applied MPE to study:
- Spectroscopic properties and images of stained DNA
- Tyrosine, Tryptophon and Proteins
- DPH-labeled membranes
- Detection of anti-cancer drugs in blood
- Ru and Re metal ligand complexes
We also demonstrated a two-color two-photon excitation with synchronized UV and NIR beams. Recently we used MPE to observe a UV fluorescence from saturated hydrocarbons and cholesterol analogues. We also applied MPE in High Throughput Screening (HTS).