The goal of the Target Validation & Screening (TVS) group is to provide investigators access to automated liquid handling equipment, sensitive high throughput detectors, and diverse collections of screening libraries to facilitate the identification of therapeutic targets and screen for chemical perturbagens as a step towards developing new drugs and therapies. The TVS program provides the expertise in cell-based or in vitro assay development and will assist the adaptation of an investigator’s existing assay into higher throughput formats. The TVS can train users on the operation of the automated liquid handling workstations and the multimode microplate readers, or the TVS personnel can be contracted to perform all aspects of a screening project if necessary. The TVS program occupies modern laboratory space in the Institute for Biosciences and Biotechnology Research (IBBR), a three-building complex of research laboratories at the University of Maryland College Park, Shady Grove in Rockville, MD and additional space at the University of Maryland School of Medicine in Baltimore, MD. It shares the facilities, equipment, and expertise with the other six programs in the Center for Biomolecular Therapeutics (CBT). The TVS group has dedicated tissue culture facilities and is capable of maintaining cell lines, creating new genetically altered lines to overexpress, knockdown via shRNA/siRNA, or knockout genes via targeted homologous recombination. The program regularly performs molecular biology techniques (RNA/DNA purification, RT-PCR, cloning, stable and transient gene expression/knockdown in mammalian cells) for characterization of cells lines. It works closely with the Protein Production & Biophysics (PPB) program to make recombinant proteins for in vitro assays and in assay development using biochemical techniques (FPLC, recombinant protein expression, chemical labeling, SDS-PAGE).