Microarrays

The Facility now offers Microarray analysis using an Affymetrix platform which includes the model 3000 scanner capable of reading features as small as 3 microns. This state of the art system accommodates any of the current high-density Affymetrix arrays, including Affymetrix's 100K mapping arrays which can be used to genotype over 100,000 SNPs in a single chip. To help maintain a high level of quality control, the Facility will QC all RNA's to be used in this service. We also maintain the instrumentation necessary to print and analyze our own custom oligonucleotide arrays, quite useful for small, directed array analysis. For custom spotted microarrays, the facility has a Cartesian microarray printer and for scanning spotted slides there are two microarray readers (or scanners), one with 4 lasers (GSI luminomics 5000) and one with 2 lasers (Axon 4000) both with 5 micron resolution. The facility has recently purchased an Applied Biosystems 7900HT high throughput real-time PCR machine for validation of gene expression data obtained from microarray experiments.