Cell Line Authentication
Increasingly, verification of cell lines is required by funding entities and
professional journals prior to acceptance of research papers and grant proposals.
The Biopolymer/Genomics Core Facility offers human cell line authentication
and detection of cell line cross-contamination using short tandem repeat (STR). STR profiling is a rapid, reproducible, and standardized PCR-based method for the authentication of human cell lines. DNA profiling includes all the standard American Type Culture Collection (ATCC) markers for cell line authentication. Our laboratory uses GenePrint® 5X Mouse Primer Pair Mix is designed to be used as a sensitive marker that specifically detects the presence of mouse (Mus musculus) DNA while simultaneously providing detection of about 1% fraction of mouse contaminant in a human cell line when using extracted DNA . The GenePrint® 10 System allows co-amplification and three-color detection of nine human loci, including the ASN-0002 loci (TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317 and D5S818) as well as D21S11. These loci collectively provide a genetic profile with a random match probability of 1
in 2.92 × 109.
Investigators provide the facility with a cell pellet or DNA; our facility will perform the extraction if a cell pellet was provided. The testing process includes PCR reaction, detection, and analysis to provide the investigator with an allele table. If a pair of samples is provided for comparison, an interpretation of the result will be issued.
- Extracted DNA - 10-1000 μl at a minimum of 10 ng/μl
- Cell Pellet
- STR Allele Report
- Results in 3-7 business days depending on sample volume
- $85 per sample for single reactions
- $100.00 per sample for DNA extraction and detection
For more information or questions, please contact April Batts 410-706-3339 or firstname.lastname@example.org