The Biopolymer Laboratory
Department of Microbiology/Immunology
Phone 410-706-8553  Fax 410-706-0287
E-mail:  biopolym@umaryland.edu

Biopolymer-Lab Sequence No.                            

DNA SEQUENCING ORDER FORM

Principal Investigator:                                                                   Date:                         
Department and School:                                                                                                   
E-Mail Address:                                                                                                                 
Name of Requestor: (if other than P.I.)                                                                          
Phone:                             Account to be charged:                                                            
Signature of P.I. or authorized person:                                                                          

** TEMPLATES BUST BE AT 0.2µg/µl IN WATER **
*** PRIMERS (IF SUBMITTED) MUST BE 1µM ***

  RUN SPECIFIED PRIMERS ONLY
  COMPLETE SINGLE STRANDED SEQUENCE
  COMPLETE DOUBLE STRANDED SEQUENCE

Template (check one box) (260/280 data required):
  Single Stranded (e.g. M13)            Plasmid (ds)            PCR

Template Name:                                                                                                                
Insert (or fragment) size:                                                                                                   
Cloning vector:                                            Cloning site:                                                 
Volume (µl)                      A260                       A280                           Dilution               

Please make sure that all tubes are clearly marked with name and date!

Primer (check one box)
  Universal Forward           Universal Reverse
  SP6                 T7           T3
  Custom Primer Name (submitted at 1µM)                                                           
  Custom Primer to be synthesized (please attach Primer Order Form)

Charges

_____

runs at $25/run _______
_____ primers at $20/primer _______
_____ centricon  $10 each sample _______
_____ OD (if not provided) $2 each _______

Total

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