M.D., 1978-1985, Medicine & Biophysics, Russian State Medical University, Moscow
Ph.D., 1990-1994, Epidemiology & Immunology, Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow
Postdoctoral Fellowship, 1996-2002, Mayo Clinic, Department of Immunology
Svetlana Chapoval, MD, PhD, is Assistant Professor in the Department of Microbiology & Immunology, and the Center for Vascular and Inflammatory Diseases, and a member of Program in Oncology, Greenebaum Cancer Center. Dr. Chapovala's research focuses on the role of immune semaphorins, Sema4A and Sema4D, in the pathogenesis of allergic airway inflammation. The overall goal of her laboratory is to define the mechanism of immune semaphorin function in the lung and further manipulate Sema4-receptor interaction and signaling in order to develop novel therapies for asthma and other lung inflammatory diseases including cancer.
Lab Techniques and Equipment
Animal models of experimental asthma, transgenic and knockout mice, genotyping, ELISA, PCR, RT-qPCR, multicolor flow cytometry and cell sorting, IHC, TUNEL, EMSA, cell proliferation and inhibition of proliferation assays, cytokine CBA, ELISPOT, AHR measurements, bone marrow transfer,Western blot, Immunoblot, small molecule inhibitors, lung morphometry, DC migration assays, in vitro Th1 and Th2 cell polarization
Heller NM, Dasgupta P, Dorsey NJ, Chapoval SP, and Keegan AD. The Type I and Type II receptor complexes for IL-4 and IL-13 differentially regulate allergic lung inflammation. In : "Allergic Diseases - Highlight in the Clinic, Mechanims and Treatment". In-tech, 2012. Edited by Celso Pereira, ISBN 978-953-51-0227-4.
Chapoval SP. Flow cytometry-based analysis and sorting of lung dendritic cells. In: "Flow Cytometry - Recent Perspectives". In-tech. 2012. Edited by Ingrid Schmid, ISBN 978-953-51-0626-5.
Atamas SP, SP Chapoval, AD Keegan. 2013. Cytokines in chronic respiratory diseases. Faculty of 1000 Biology Reports, 5:3 (doi: 10.3410/B5-3).
Nkyimbeng-Takwi EH, K Shanks, Smith EP, A Iyer, MM Lipsky, LJ DeTolla, H Kikutani, AD Keegan, SP Chapoval. 2012. Neuroimmune semaphorin 4A downregulates the severity of allergic response. Mucosal Immunology. 5:409-419.
Dasgupta P, SP Chapoval, EP Smith, AD Keegan. 2011. Transfer of in vivo primed transgenic T cells supports allergic lung inflammation and FIZZ1 and Ym1 production in an IL-4RÎ± and STAT6 dependent manner. BMC Immunology. 12:60.
Smith EP, K Shanks, MM Lipsky, LJ DeTolla, AD Keegan, SP Chapoval. 2011. Expression of neuroimmune semaphorins 4A and 4D and their receptors in the lung is enhanced by allergen and vascular endothelial growth factor. BMC Immunology. 12:30.
Nkyimbeng-Takwi EH and SP Chapoval. 2011. Biology and function of neuroimmune semaphorins 4A and 4D. Immunologic research. 50:10-21.
Chapoval SP, P Dasgupta, EP Smith, LJ DeTolla, MM Lipsky, A Kelly-Welch, AD Keegan. 2011. STAT6 expression in multiple cell types mediates the cooperative development of allergic airway disease. J. Immunol. 186:2571-2583.
Gunsolly, C., Nicholson, J.D., Listwak, S.J., Ledee, D., Zelenka, P., Verthelyi, D., Chapoval, S., Keegan, A., Tonelli, L.H. (2010). Expression and regulation in the brain of the chemokine CCL27 gene locus. Journal of Neuroimmunology. 225(1-2):82-90. PMCID: PMC2924910
Chapoval, S.P., Dasgupta, P., Dorsey, N.J., Keegan, A.D. (2010). Regulation of the T helper cell Type 2 (Th2)/ T regulatory cell (Treg) balance by IL-4 and STAT6. Journal of Leukocyte Biology. 87:1011-1018. PMCID: PMC2872539
Chapoval, S.P., Lee, C.G., Tang, C., Keegan, A.D., Cohn, L., Bottomly, K., Elias, J.A. (2009). Lung vascular endothelial growth factor expression induces local myeloid dendritic cell maturation. Clinical Immunololgy. 132(3):371-384. PMCID: PMC2780370
Yamasaki, M., Kang, H.R., Homer, R.J., Chapoval, S.P., Cho, S.J., Lee, B.J., Elias, J.A., Lee, C.G. (2008). P21 regulates TGF-beta1-induced pulmonary responses via a TNF-alpha-signaling pathway. American Journal of Respiratory Cell and Molecular Biology. 38(3):346-353. PMCID: PMC2258454
Chapoval, S.P., Al-Garawi, A., Lora, J.M., Strickland, I., Ma, B., Lee, P.J., Homer, R.J., Ghosh, S., Coyle, A.J., Elias, J.A. (2007). Inhibition of NF-kappaB activation reduces the tissue effects of transgenic IL-13. Journal of Immunology. 179(10):7030-7041. PMID: 17982094
Chapoval SP, A Al-Garawi, JM Lora, I Strickland, B Ma, PJ Lee, RJ Homer, S Ghosh, AJ Coyle, JA Elias. 2007. Inhibition of NF-kB Activation Reduces the Tissue Effects of Transgenic IL-13. J. Immunol. Accepted for publication.
Bhandari V, Choo-Wing R, Chapoval SP, Lee CG, Tang C, Kim YK, Ma B, Baluk P, Lin MI, McDonald DM, Homer RJ, Sessa WC, Elias JA. Essential role of nitric oxide in VEGF-induced, asthma-like angiogenic, inflammatory, mucus, and physiologic responses in the lung. Proc Natl Acad Sci U S A. 2006;103:11021-6.
Ma B, CG Lee, S Chapoval, W Liu, A Coyle, J Lora, D Picarella, RJ Homer, JA Elias. Role of CCR5 in gamma-interferon-induced and cigarette smoke-induced emphysema. J. Clin. Invest. 2005; 115:3460-3472.
Xue A, SP Chapoval, ES Finn, VR Chowdhary, EV Marietta, TA Gaffey, AC Pursifull, CS David. HLA-DQ8 is a predisposing molecule for detergent enzyme subtilisin BPN-induced hypersensitivity. Clin. Immunol. 2005;117:302-315.
Lee CG, H Link, P Baluk, RJ Homer, S Chapoval, V Bhandari, MJ Kang, L Cohn, YK Kim, DM McDonald, and JA Elias. Vascular endothelial growth factor (VEGF) induces remodeling and enhances Th2-mediated sensitization and inflammation in the lung. Nature Med. 2004; 10:1095-1103.
Lee CG, SJ Cho, MJ Kang, SP Chapoval, PJ Lee, PW Noble, T Yehualaeshet, B Lu, RA Flavell, J Milbrandt, RJ Homer, JA Elias. Early growth response gene 1-mediated apoptosis is essential for transforming growth factor beta1-induced pulmonary fibrosis. 2004; J. Exp. Med. 200:377-389.
Das P, S Chapoval, V Howard, CS David and TE Golde. Immune response against Aï¢1-42 in HLA class II transgenic mice: implications for Aï¢1-42 immune-mediated therapies. Neurobiol. Aging. 2003; 24: 969-976.
Chapoval SP and CS David. Identification of antigenic epitopes on human allergens: Studies with HLA transgenic mice. Environ. Health Perspect. 2003; 111:245-250.
Chapoval SP and CS David. CD28 costimulation is critical for experimental allergic asthma in HLA-DQ8 transgenic mice. Clin. Immunol. 2003; 106:83-94.
Chapoval SP, EV Marietta, MK Smart, K Iijima, AI Chapoval, AG Andrews, and CS David. Allergic inflammatory response to short ragweed extract in HLA-DQ transgenic mice lacking CD4 gene. J. Immunol. 2002; 168:890-899.
Papouchado BG, SP Chapoval, EV Marietta, CR Weiler, CS David. Cockroach allergen-induced eosinophilic airway inflammation in HLA-DQ/human CD4+ transgenic mice. J. Immunol. 2001; 167: 4627-4634.
Chapoval SP, EV Marietta, MK Smart, CS David. Requirements for allergen-induced airway inflammation and hyperreactivity in CD4-deficient and CD4-sufficient human leukocyte antigen-DQ transgenic mice. J. Allergy Clin. Immunol. 2001; 108:764-771.
Krco CJ, J Harders, S Chapoval, and CS David. Immune response of HLA-DQ transgenic mice to house dust mite allergen p2: Identification of HLA-DQ restricted minimal epitopes and critical residues. Clin. Immunol. 2000; 97:154-161.
Papouchado BG, SP Chapoval, EV Marietta, CR Weiler, CS David. HLA-DQ/human CD4-restricted immune response to cockroach allergens in transgenic mice. Tissue Antigens. 2000; 55:303-311.
Chapoval SP, GH Nabozny, EV Marietta, EL Raymond, CJ Krco, AG Andrews, and CS David. Short ragweed allergen induces eosinophilic lung disease in HLA-DQ transgenic mice. J. Clin. Invest. 1999; 103:1707-1717.
Chapoval SP, T Neeno, CJ Krco, E Marietta, J Harders, and CS David. HLA-DQ6 and HLA-DQ8 transgenic mice respond to ragweed allergens and recognize distinct set of epitopes on short and giant ragweed group 5 antigens. J. Immunol. 1998. 161: 2032-2037.