Microbiology and Immunology
Postdoctoral, Program in Oncology
1998: BS (Microbiology, Biochemistry & food and nutrition), Nagarjuna University, Guntur, India
2000: MS (Biotechnology), Hyderabad Central University, Hyderabad, India
2006: Ph.D (Biochemistry),Hyderabad Central University, Hyderabad, India
Regulation of novel IFN-induced Cell death-activating genes, Autophagy, Apoptosis and ER stress
Lab Techniques and Equipment:
The researcher has employed all standard molecular biological techniques including cloning, transfection, mutational analysis, gene expression analysis, promoter assays, expression of recombinant proteins, protein:protein interactions, autophagy & apoptosis assays, DNA binding assays such as ChIP assays, RNAi, reconsitution of gene expression in mammalian cells following RNAI, lentiviral gene expression systems, knockout cell lines and kncokout mice, induction of tumor carcinogenesis in mice.
Gade, P., Ramachandran, G., Maachani, UB., Rizzo, MA., Okada, T., Prywes, R., Cross, AS., Mori, K., and Kalvakolanu, DV (2012). An IFN-gamma-stimulated ATF6-C/EBP-beta-signaling pathway critical for the expression of Death Associated Protein Kinase 1 and induction of autophagy. Proc Natl Acad Sci U S A 109: 10316-10321.
Gade, P., Roy, S. K., Li, H., Nallar, S. C., and Kalvakolanu, D. V. 2008. Critical role for transcription factor C/EBP-beta in regulating the expression of death-associated protein kinase 1. Mol Cell Biol 28:2528-48.
Gade, P, Singh, A. K., Roy, S. K., Reddy, S. P., and Kalvakolanu, D. V. 2009. Down-regulation of the transcriptional mediator subunit Med1 contributes to the loss of expression of metastasis-associated dapk1 in human cancers and cancer cells. Int J Cancer 125:1566-74.
Gade, P, and Kalvakolanu, D. V. 2010. Chromatin immuno-precipitation (ChIP) Assay as tool for analyzing transcription factor activity. Methods in Molecular Biology 809:85-104.