Research Images:Human mammary epithelial cells (MCF10A) attaching to extracellular matrix. Cells are stained fluorescently for actin (red), microtubules (green) and DNA (blue). Dynamic rearrangement of the actin cytoskeleton is essential for both cell spreading and survival.
In vivo imaging of transplanted breast tumor cell lines in a single mouse. Real-time tracking and measurement of cells is possible for those tumors that grow (MEK/Bcl2) as well as those that lie dormant (EpH4 or Bcl2). At these early time points, none of the tumors are palpable, but clear differences can still be detected by bioluminescence after injection of luciferin.
Lab Techniques and Equipment: