- 1972-1976, B.S., Microbiology/Chemistry, University of Illinois, Urbana, IL
- 1976-1981, Ph.D., Immunology. Wayne State University, Detroit, MI
- 1981-1986, Post-doctoral Fellow, Immunogenetics, NIAID, NIH, Bethesda, MD
- 1986-1988, Research Scientist, Frederick Cancer Research Facility, Frederick, MD
- 1988-1992, Assistant Director, Cambridge Biotech Corp., Rockville, MD
- 1992-2000, Director of Research, Dexall Biomedical Labs, Gaithersburg, MD
- 2000-2005, Assistant Professor, Department of Pharmaceutical Sciences, UMB School of Pharmacy
- 2001-present, Graduate Faculty, UMB Graduate School
- 2005-2008, Assistant Professor, Department of Pediatrics, UMB School of Medicine
- 2008-present, Associate Professor, Department of Pediatrics, UMB School of Medicine
Our laboratory studies the interactions of microbial pathogens with the respiratory and gastrointestinal mucosal surfaces. Two model systems are currently being explored: (1) expression, structure, and function of MUC1 mucin, and (2) intestinal immunity against coccidiosis.
Expression, structure, and function of MUC1 mucin by respiratory and gastric epithelial cells. Mucin-1 (MUC1) belongs to a family of mucin glycoproteins that are divided into secreted and membrane-bound forms. MUC1 is a membrane mucin expressed by mucosal epithelial cells, and overexpressed by most carcinomas. Unlike other membrane mucins, the cytoplasmic region of MUC1 contains multiple sites of serine and tyrosine phosphorylation that are associated with a wide array of cellular processes, including the host response to pathogenic microorganisms, cell growth, cell differentiation, intercellular adhesion, and apoptosis. In collaboration with scientists at Temple University School of Medicine and the University of Maryland School of Medicine, we are currently investigating the role of MUC1 in: (1) Pseudomonas aeruginosa lung infection, (2) Helicobacter pylori gastric infection, (3) intracellular signal transduction, and (4) epithelial injury, repair, and restitution. Through these studies, we hope to glean new information relevant to understanding the pathogenesis of lung diseases such as cystic fibrosis and gastric disorders such as peptic ulcer disease and stomach cancer.
Intestinal immunity against coccidiosis. Coccidiosis is an intestinal disease caused by Eimeria spp. that impairs the feed utilization and growth of commercial poultry. Anti-coccidial antibiotics are currently used to control outbreaks of infection, but parasite drug resistance and regulatory restrictions on antibiotic use in livestock have spawned an interest in alternative strategies for disease control. In collaboration with scientists at the USDA, our laboratories are identifying immunogenic components of Eimeria with the goal of developing coccidiosis vaccines. In addition, we are isolating and characterizing cytokines and chemokines as vaccine adjuvants.
A third project in our laboratory is investigating the role of human endogenous retroviruses (HERVs) in the pathogenesis of acute onset schizophrenia and bipolar disease in collaboration with Dr. Robert H. Yolken, Johns Hopkins University School of Medicine. We have discovered that antibodies to exogenous simian retroviruses are present at significantly greater frequency in patients with schizophrenia or bipolar disorder compared with matched controls. We hypothesize that these antibodies are generated against endogenous retroviruses and can be detected by cross-reaction with exogenous viruses.
Current Grant Support:
Diagnostic Potential of Retrovirus Envelope Glycoproteins in Acute Onset Schizophrenia and Bipolar Disorder.
Stanley Medical Research Institute
Regulation of MUC1 Mucin During Airway Inflammation.
Most Recent Publications
Lee, K. W., Lillehoj, H. S., Jang, S. I., Pagès M., Bautista, D. A., Pope, C. R., Ritter, G. D., Lillehoj, E. P., Neumann, A. P., and Siragusa, G. R. 2012. Effects of in ovo vaccination and anticoccidials on the distribution of Eimeria spp. in poultry litter and serum antibody titers against coccidia in broiler chickens raised on the used litters. Res. Vet. Sci. 93:177-182.
Kyo, Y., Kato, K., Park, Y. S., Gajhate, S., Umehara, T., Lillehoj, E. P., Suzaki, H., and Kim, K. C. 2012. Anti-inflammatory role of MUC1 mucin during infection with nontypeable Haemophilus influenzae. Am. J. Respir. Cell Mol. Biol. 46:149-156.
Lee, S. H., Lillehoj, S. H., Jang, S. I., Lee, K. W., Baldwin, C., Tompkins, D., Wagner, B., del Cacho, E., Lillehoj, E. P., and Hong, Y. H. 2012. Development and characterization of mouse monoclonal antibodies reactive with chicken CD83. Vet. Immunol. Immunopathol. 145:527-533.
Kato, K., Lillehoj, E. P., Park, Y. S., Umehara, T., Hoffman, N. E., Muniswamy, M., and Kim, K. C. 2012. Membrane-tethered MUC1 Mucin is phosphorylated by EGFR in airway epithelial cells and associates with TLR5 to inhibit recruitment of MyD88. J. Immunol. 188:2014-2022.
Lillehoj, E. P., Hyun, S. W., Feng, C., Zhang, L., Liu, A., Guang, W., Nguyen, C., Luzina, I. G., Atamas, S. P., Passaniti, A., Twaddell, W. S., Puché, A. C., Wang, L. X., Cross, A. S., and Goldblum, S. E. 2012. NEU1 sialidase expressed in human airway epithelia regulates epidermal growth reactor receptor (EGFR) and MUC1 signaling. J. Biol. Chem. 287:8214-8231.
Park, Y. S., Lillehoj, E. P., Kato, K., Park, C. S., and Kim, K. C. 2012. PPARγ inhibits airway epithelial cell inflammatory response through a MUC1-dependent mechanism. Am. J. Physiol. Lung Cell. Mol. Physiol. 302:L679-L687.
Lee, K. W., Hong, Y. H., Lee, S. H., Jang, S. I., Park, M. S., Bautista, D. A., Ritter, G. D., Jeong W., Jeoung, H. Y., An, D. J., Lillehoj, E. P., and Lillehoj, H. S. 2012. Effects of anticoccidial and antibiotic growth promoter programs on broiler performance and immune status. Res. Vet. Sci. 93:721-728.
del Cacho, E., Gallego, M., Lee, S. H., Lillehoj, H. S., Quilez, J., Lillehoj, E. P., and Sánchez-Acedoa, C. 2012. Induction of protective immunity against Eimeria tenella, Eimeria maxima, and Eimeria acervulina infections using DC-derived exosomes. Infect. Immun. 80:1909-1916.
Kim, D. K., Lillehoj, H. S., Lee, S. H., Lillehoj, E. P., and Bravo, D. 2012. Improved resistance to Eimeria acervulina infection in chickens due to dietary supplementation with garlic metabolites. Br. J. Nutr. 13:1-13.
Cross, A. S., Hyun, S. W., Miranda-Ribera, A., Feng, C., Liu, A., Nguyen, C., Zhang, L., Luzina, I. G., Atamas, S. P., Twaddell, W. S., Guang, W., Lillehoj, E. P., Puché, A. C., Huang, W., Wang, L. X., Passaniti, A., and Goldblum, S. E. 2012. NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia. NEU1 restrains endothelial cell migration whereas NEU3 does not. J. Biol. Chem. 287:15966-15980.
Dickerson, F., Lillehoj, E. P., Stallings, C., Wiley, M., Origoni, A., Vaughan, C., Khushalani, S., Sabunciyan, S., and Yolken, R. 2012. Antibodies to retroviruses in recent onset psychosis and multi-episode schizophrenia. Schizophr. Res. 136:128-136.
Lee, K. W., Lillehoj, H. S., Jang, S. I., Li, G. X., Bautista, D. A., Phillips, K., Ritter, G. D., Lillehoj, E. P., and Siragusa, G. R. 2012. Effects of coccidiosis control programs on antibody levels against selected pathogens and serum nitric oxide levels in broiler chickens. J. Appl. Poultr. Res. 20:143-152.
Guang, W., Twaddell, W. S., and Lillehoj, E. P. 2012. Molecular interactions between MUC1 epithelial mucin, β-catenin, and CagA proteins. Front. Immunol. 3:105.
Kim, D. K., Lillehoj, H. S., Lee, S. H., Dominowski, P., Yancey, R. J., and Lillehoj, E. P. 2012. Effects of novel vaccine/adjuvant complexes on the protective immunity against Eimeria acervulina and transcriptome profiles. Avian Dis. 56:97-109.
Lee, K. W., Lillehoj, H. S., Park, M. S., Jang, S. I., Ritter, G. D., Hong, Y. H., Jeong, W., Jeoung, H. Y., An, D. J., and Lillehoj, E. P. 2012. Clostridium perfringens alpha-toxin and NetB toxin antibodies and their possible role in protection against necrotic enteritis and gangrenous dermatitis in broiler chickens. Avian Dis. 56:230-233.
Umehara, T., Park, Y. S., Kato, K., Lillehoj, E. P., Kawauchi, H., and Kim, K. C. 2012. Prevention of lung injury by Muc1 mucin in a mouse model of repetitive Pseudomonas aeruginosa infection. Inflamm. Res. 61:1013-1020.
Jang, S. I., Lillehoj, H. S., Lee, S. H., Lee, K. W., Lillehoj, E. P., Hong, Y. H., An, D. J., Jeong, W., Chun, J. E., Bertrand, F., Dupuis, L., Deville, S., and Ben Arous, J. 2012. Vaccination with Clostridium perfringens recombinant proteins in combination with MontanideTM ISA 71 VG adjuvant increases protection against experimental necrotic enteritis in commercial broiler chickens. Vaccine 30:5401-5406.
Park, Y. S., Guang, W., Blanchard, T. G., Kim, K. C., and Lillehoj, E. P. 2012. Suppression of IL-8 production in gastric epithelial cells by MUC1 mucin and peroxisome proliferator-associated receptor-γ. Am. J. Physiol. Gastrointest. Liver Physiol. 303:G765-G774.
Lee, S. H., Lillehoj, H. S., Jang, S. I., Lee, K. W., Kim, D. K., Lillehoj, E. P., Yancey, R. J., and Dominowski, P. J. 2012. Evaluation of novel adjuvant Eimeria profilin complex on intestinal host immune responses against live E. acervulina challenge infection. Avian Dis. 56:402-405.
Kim, D. K., Lillehoj, H. S., Lee, K. W., Jang. S. I., Neumann, A. P., Siragusa, G. R., Lillehoj, E. P., and Hong, Y. H. 2012. Genome-wide differential gene expression profiles in broiler chickens with gangrenous dermatitis. Avian Dis. 56:670-679.
Lillehoj, E. P., Kato, K., Lu, W., and Kim, K. C. 2013. Cellular and molecular biology of airway mucins. Int. Rev. Cell Mol. Biol. 303:139-202.
Jang, S. I., Kim, D. K., Lillehoj, H. S., Lee, S. H., Lee, K. W., Bertrand, F., Dupuis, L., Deville, S., Ben Arous, J., and Lillehoj, E. P. 2013. Evaluation of the ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis. PLoS One 8: e59786.
Lee, S. H., Lillehoj, H. S., Jang, S. I., Lillehoj, E. P., Min, W., and Bravo, D. M. 2013. Dietary supplementation of young broiler chickens with Capsicum and turmeric oleoresins increases resistance to necrotic enteritis. Brit. J. Nutr., in press.
Lee, K. W., Lillehoj, H. S., Jang, S. I., Lee, S. H., Bautista, D. A., Ritter, G. D., Lillehoj, E. P., and Siragusa, G. R. 2013. Comparison of live Eimeria vaccination with in-feed salinomycin on growth and immune status in broiler chickens. Res. Vet. Sci., in press.
Lee, S. H., Lillehoj, H. S., Jang, S. I., Jeong, M., Tuo, W., Murphy, C. A., Hong, Y. H., and Lillehoj, E. P. 2013. Parasiticidal activity of a novel synthetic peptide from the core α-helical region of NK-lysin. Vet. Parasitol., in press.
Jang, S. I., Lillehoj, H. S., Lee, S. H., Lee, K. W., Lillehoj, E. P., Hong, Y. H., An, D. J., Jeoung, H. Y., and Chun, J. E. 2013. Relative disease susceptibility and clostridial toxin antibody responses in three commercial broiler lines coinfected with Clostridium perfringens and Eimeria maxima using an experimental model of necrotic enteritis. Avian Dis., in press.
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