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Lai-Xi  Wang
 

Lai-Xi Wang Ph.D.

Academic Title: Professor
Primary Appointment: Biochemistry and Molecular Biology
Secondary Appointments: Medical and Research Technology
LWang@som.umaryland.edu
Location: 725 W. Lombard St., S-518
Phone: (410) 706-4982
Lab: (410) 706-4983

Personal History:

Education
 
  • PhD, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
  • Postdoctoral Fellow, Johns Hopkins University
  • Postdoctoral Associate, Massachusetts Institute of Technology
Professional Experience
 
  • 2000-2005 Assistant Professor (Tenure Track), Institute of Human Virology, University of Maryland Biotechnology Institute (UMBI), University of Maryland
  • 2005-2009 Associate Professor (Tenured), Institute of Human Virology, UMBI and University of Maryland School of Medicine
  • 2007-2009 Associate Professor (Tenured), Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine
  • 2006-present Member, The Greenebaum Cancer Center, University of Maryland
  • 2009-present Professor (Tenured), Institute of Human Virology, University of Maryland School of Medicine
  • 2009-present Professor, Department of Medical Research & Technology, University of Maryland School of Medicine
  • 2009-present Professor (Tenured), Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine
Other activities and honors

  • 2008-2012, Chartered Member, NIH Synthetic and Biological Chemistry (SBCA) Study Section
  • 2005-2007, Ad Hoc Member, NIH Synthetic and Biological Chemistry (SBCA) Study Section
  • 2006-2008, Member, NIH BCMB Special Emphasis Panel
  • Member, Editorial Advisory Board, "Recent Patent Reviews on Anti-Infective Drug Discovery"
  • Executive Editor for special issues, Current Pharmaceutical Design (Bentham Science Publishers)
  • The 2004 Young Investigator Award in Carbohydrate Chemistry, The American Chemical Societ
  • 2009 Inducted to Johns Hopkins University Society of Scholars
  • Member of the Editorial Board, “Carbohydrate Research”

Research Interests:

My research group is working in the areas of bioorganic chemistry, glycobiology, and immunology. The focus of our current research is centered on protein glycosylation, one of the most ubiquitous posttranslational modifications of proteins in eukaryotes. It is estimated that half of total human proteins are glycoproteins. Thus, glycosylation adds a new level of structural and functional diversity of proteins and expands the biological information of an otherwise concise human genome. We combine synthetic organic chemistry, structural biology, and immunology to understand how glycosylation affects a protein's structure and function. Three research projects are going on in my laboratory: 1) Development of chemoenzymatic methods for glycopeptide and glycoprotein synthesis; 2) Structural and mechanistic study of endoglycosidases and glycosynthases; and 3) chemical glycobiology of HIV with an emphasis on exploring the envelope glycoproteins for HIV vaccine design.

Publications:

Selected Publications

Huang, W., Yang, Q., Umekawa, M., Yamamoto, K., Wang, L. X.*, “Arthrobacter endo-beta-N-acetylglucosaminidase shows transglycosylation activity on complex type N-glycan oxazolines. One-pot conversion of ribonuclease B to sialylated ribonuclease C”. ChemBioChem, in press (2010).

Brooks, C. L., Schietinger, A., Borisova, S. N., Kufer, P., Okon, M., Hirama, T., MacKenzie, C. R., Wang, L. X., Schreiber, H., Evans, S. V., “Antibody recognition of a novel tumor-specific glycopeptide antigen”, Proc. Natl. Acad. Sci. USA, 107, 10056-10061 (2010).

Yang, Q., Li, C., Wei, Y., Huang, W., Wang, L. X.*, “Expression, glycoform characterization, and antibody-binding of HIV-1 V3 glycopeptide domain fused with human IgG1-Fc”, Bioconjugate Chem., 21, 875-883 (2010).

Schwarz, F., Huang, W., Li, C., Schulz, B. L., Lizak, C., Palumbo, A., Numao, S., Neri, D., Aebi, M.*, Wang, L. X.*, “A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation”, Nat. Chem. Biol., 6, 264-266 (2010).

Umekawa, M., Li, C., Higashiyama, T., Huang, W., Ashida, H., Yamamoto, K.*, Wang, L. X.*, “Efficient glycosynthase mutant derived from Mucor hiemalis endo-beta-N-acetylglucosaminidase capable of transferring oligosaccharide from both sugar oxazoline and natural N-glycan”, J. Biol. Chem., 281, 511-521 (2010).

Huang, W., Wang, D., Yamada, M., Wang, L. X.*, “Chemoenzymatic synthesis and lectin array characterization of a class of N-glycan clusters”, J. Am. Chem. Soc., 131, 17963-17971 (2009).

Huang, W., Li, C., Li, B., Umekawa, M., Yamamoto, K., Zhang, X., Wang, L. X.*, “Novel glycosynthases enable a highly efficient chemo-enzymatic synthesis of N-glycoproteins carrying intact natural N-glycans”, J. Am. Chem. Soc., 131, 2214-2223 (2009).

Zhu, X. Y., Holtz, B., Wang, Y., Wang, L. X., Orndorff, P. E., Guo, A., “Quantitative glycomics from fluidic glycan microarrays”, J. Am. Chem. Soc., 131, 13646-13650 (2009).

Wang, L. X.*, “Expanding the repertoire of glycosynthases”, Chem. Biol., 16, 1026-1027 (2009).

Newsom-Davis, T. E., Wang, D., Steinman, L., Chen, P. F. T., Wang, L. X., Simon, A. K., Screaton, G. R., “Enhanced immune recognition of cryptic glycan markers in human tumours”, Cancer Research., 69, 2018-25 (2009).

Wang, L. X.*, Huang, W., “Enzymatic transglycosylation for glycoconjugate synthesis”, Current Opinion in Chemical Biology, 13, 592–600 (2009).

Huang, W., Groothuys S., Heredia A., Kuijpers B.H., Rutjes F.P., van Delft F.L.*, Wang, L. X.*, “Enzymatic glycosylation of triazole-linked GlcNAc/Glc-peptides: Synthesis, stability and anti-HIV activity of triazole-linked HIV-1 gp41 glycopeptide C34 analogues”, ChemBioChem, 10, 1234-1242 (2009).

Ochiai, H., Huang, W., Wang, L. X.*, “Endo-beta-N-acetylglucosaminidase catalyzed polymerization of Glc-beta-(1→4)-GlcNAc oxazoline: A revisit to the enzymatic transglycosylation”, Carbohydr. Res., 344, 592-8 (2009).

Luallen, R. J., Fu, H., Agrawal-Gamse, C., Mboudjeka, I., Huang, W., Lee, F. H., Wang, L. X., Doms, R. W., Geng, Y., “A yeast glycoprotein shows high affinity binding to the broadly neutralizing HIV antibody 2G12 and effectively disrupts gp120 interactions with 2G12 and DC-SIGN”, J. Virol., 83, 4861-70 (2009).

Yin, J., Li, L., Shaw, N., Li, Y., Song, J. K., Zhang, W., Xia, C., Zhang, R., Joachimiak, A., Wang, L. X., Liu, Z. J., Wang, P. G., “Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A”, PLoS ONE, 4(3):e4658 (2009).

Lin, W., Voskens, C.J., Zhang, X., Schindler, D.G., Wood, A., Burch, E., Wei, Y., Chen, L., Tian, G., Tamada, K., Wang, L. X.,Schulze, D.H., Mann, D., Strome, S.E., “Fc dependent expression of CD137 on human NK cells: insights into "agonistic" effects of anti-CD137 monoclonal antibodies”, Blood, 112, 699-707 (2008).

Huang, W., Ochiai, H., Zhang, X., Wang, L. X.*, “Introducing N-glycans into natural products through a chemoenzymatic approach”, Carbohydr. Res., 343, 2903-2913 (2008).

“Glyco-engineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation”, Biochemistry, 47, 10294-10304 (2008).

Ochiai, H., Huang, W., Wei, Y., Wang, L. X.*, “Expeditious chemoenzymatic synthesis of homogeneous N-glycoproteins carrying defined oligosaccharide ligands”. J. Am. Chem. Soc., 130, 13790-13803 (2008).

Li, C., Huang, W., Wang, L. X.*, “Chemoenzymatic synthesis of N-linked neoglycoproteins through a chitinase-catalyzed transglycosylation”, Bioorg. Med. Chem., 16, 8366-8372 (2008).

Li, B., Takegawa, K., Suzuki, T., Yamamoto, K., Wang, L. X.*, “Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-beta-N-acetylglucosaminidases”, Bioorg. Med. Chem., 16, 4670-4675 (2008).

Wang, L. X.*, “Chemoenzymatic synthesis of glycopeptides and glycoproteins through endoglycosidase-catalyzed transglycosylation”, Carbohydr. Res., 343, 1509-22 (2008).

Umekawa, M., Huang, W., Li, B., Fujita, K., Ashida, H., Wang, L. X.*, Yamamoto, K.*, “Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities”, J. Biol. Chem., 283, 4469-4479 (2008).

Li, X., Wang, L. X., Wang, X., Roseman, S., “The chitin catabolic cascade in the marine bacterium Vibrio Cholerae: Characterization of a unique chitin oligosaccharide deacetylase”, Glycobiology, 17, 1377-1387 (2007).

Li, H. G., Guan, Y., Szczepanska, A., Moreno-Vargas, A. J., Carmona, A. T., Robina, I., Lewis, G. K., Wang, L. X.*, “Synthesis and anti-HIV activity of trivalent CD4-mimetic miniproteins”, Bioorg. Med. Chem, 15, 4220-4228 (2007).

Wang, J., Li, H., Zou, G., Wang, L. X.*, “Novel template-assembled oligosaccharide clusters as epitope mimics for HIV-neutralizing antibody 2G12. Design, synthesis, and antibody binding study”, Org. Biomol. Chem., 5, 1529-1540 (2007).

Wang, L. X.*, “Toward oligosaccharide- and glycopeptide-based HIV vaccines” Current Opinion in Drug Discovery and Development, 9, 194-206 (2006).

Li, B., Song, H., Hauser, S., Wang, L. X.*, “A highly efficient chemoenzymatic approach toward glycoprotein synthesis”, Org. Lett., 8, 3081-3084 (2006)

Zeng, Y., Wang, J., Li, B., Hauser, S., Li, H., Wang, L. X.*, “Glycopeptide synthesis through endoglycosidase-catalyzed oligosaccharide transfer of sugar oxazolines. Probing substrate structural requirement. Chem. Eur. J., 12, 3355-3364 (2006).

Ni. J., Song, H., Wang, Y., Stamatos, N., Wang, L. X.*, “Toward a carbohydrate-based HIV-1 vaccine: Synthesis and immunological studies of oligomannose-containing glycoconjugates”. Bioconjugate Chem., 17, 493-500 (2006).

Li, H, Li, B., Song, H., Breydo, L., Baskakov, I. V., Wang, L. X.*, “Chemoenzymatic synthesis of HIV-1 V3 domain glycopeptides carrying two N-glycans and effects of glycosylation on the peptide’s domain”. J. Org. Chem., 70, 9990-9996 (2005).

Wang, J., Le, N., Heredia, A., Song, H., Redfield, R., Wang, L. X.*, “Modification and structure-activity relationship of a small molecule HIV-1 inhibitor targeting the viral envelope glycoprotein gp120”. Org. Biomol. Chem., 3, 1781-1786 (2005).

Stamatos, N. M., Liang, F., Nan, X., Landry, K., Cross, A. S., Wang, L. X., & Pshezhetsky, A. V. “Endogeneous sialidase activity of human monocytes is up-regulated during cellular differentiation into macrophages”. FEBS Journal, 272, 2545-2556 (2005).

Wang, L. X.*, Song, H., Liu, S., Lu, H., Jiang, S., Ni, J., Li, H. “Chemoenzymatic synthesis of HIV-1 glycopeptides. Effects of glycosylation on the antiviral activity and alpha-helix bundle-forming ability of gp41 peptide C34”. ChemBioChem, 6, 1068-1074 (2005).

Hauser, S., Song, H., Li, H., Wang, L. X.*, “A novel fluorescence-based assay for the transglycosylation activity of endo-beta-N-acetyl-glucosaminidases”. Biochem. Biophys. Res. Commun., 328, 580-585 (2005).

Li, H., Singh, S., Song, H., Zeng, Y., Wang, L. X.*, “Chemoenzymatic synthesis of CD52 glycoprotein carrying native N-glycans”. Bioorg. Med. Chem. Lett., 15, 895-898 (2005).

Li, B., Zeng, Y., Hauser, S., Song, H., Wang, L. X.*, “Highly efficient endoglycosidase-catalyzed synthesis of glycopeptides using oligosaccharide oxazolines as donor substrates”. J. Am. Chem. Soc., 127, 9692-9693 (2005).