Lai-Xi Wang
 

Lai-Xi Wang Ph.D.
Academic Title: Professor
Primary Appointment: Biochemistry and Molecular Biology
Secondary Appointments: Medical and Research Technology
lwang3@ihv.umaryland.edu
Location: 725 W. Lombard St., S-518
Phone: (410) 706-4982
Lab: (410) 706-4983

Personal History

Education
 
  • PhD, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
  • Postdoctoral Fellow, Johns Hopkins University
  • Postdoctoral Associate, Massachusetts Institute of Technology
Professional Experience
 
  • 2000-2005 Assistant Professor (Tenure Track), Institute of Human Virology, University of Maryland Biotechnology Institute (UMBI), University of Maryland
  • 2005-2009 Associate Professor (Tenured), Institute of Human Virology, UMBI and University of Maryland School of Medicine
  • 2007-2009 Associate Professor (Tenured), Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine
  • 2006-present Member, The Greenebaum Cancer Center, University of Maryland
  • 2009-present Professor (Tenured), Institute of Human Virology, University of Maryland School of Medicine
  • 2009-present Professor, Department of Medical Research & Technology, University of Maryland School of Medicine
  • 2009-present Professor (Tenured), Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine
Other activities and honors

  • 2008-2012, Chartered Member, NIH Synthetic and Biological Chemistry (SBCA) Study Section
  • 2005-2007, Ad Hoc Member, NIH Synthetic and Biological Chemistry (SBCA) Study Section
  • 2006-2008, Member, NIH BCMB Special Emphasis Panel
  • Member, Editorial Advisory Board, "Recent Patent Reviews on Anti-Infective Drug Discovery"
  • Executive Editor for special issues, Current Pharmaceutical Design (Bentham Science Publishers)
  • The 2004 Young Investigator Award in Carbohydrate Chemistry, The American Chemical Society

Research Interests

My research group is working in the areas of bioorganic chemistry, glycobiology, and immunology. The focus of our current research is centered on protein glycosylation, one of the most ubiquitous posttranslational modifications of proteins in eukaryotes. It is estimated that half of total human proteins are glycoproteins. Thus, glycosylation adds a new level of structural and functional diversity of proteins and expands the biological information of an otherwise concise human genome. We combine synthetic organic chemistry, structural biology, and immunology to understand how glycosylation affects a proteinâ?Ts structure and function. Three research projects are going on in my laboratory: 1) Development of chemoenzymatic methods for glycopeptide and glycoprotein synthesis; 2) Structural and mechanistic study of endoglycosidases and glycosynthases; and 3) chemical glycobiology of HIV with an emphasis on exploring the envelope glycoproteins for HIV vaccine design.

Publications

Selected Publications

Ochiai, H., Huang, W., Wei, Y., Wang, L. X., “Expeditious chemoenzymatic synthesis of homogeneous N-glycoproteins carrying defined oligosaccharide ligands”. Journal of American Chemical Society, 2008, 130, 13790-13803 (2008).

Huang, W., Ochiai, H., Zhang, X., Wang, L. X., “Introducing N-glycans into natural products through a chemoenzymatic approach”, Carbohydrate Research, in press (2008).

Wei, Y., Li, C., Huang, W., Li, B., Strome, S. E., Wang, L. X., “Glyco-engineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation”, Biochemistry, 47, 10294-10304 (2008).

Lin, W., Voskens, C.J., Zhang, X., Schindler, D.G., Wood, A., Burch, E., Wei, Y., Chen, L., Tian, G., Tamada, K., Wang, L.X., Schulze, D.H., Mann, D., Strome, S.E., “Fc dependent expression of CD137 on human NK cells: insights into "agonistic" effects of anti-CD137 monoclonal antibodies”, Blood, 112, 699-707 (2008).

Li, C., Huang, W., Wang, L. X., “Chemoenzymatic synthesis of N-linked neoglycoproteins through a chitinase-catalyzed transglycosylation”, Bioorganic & Medicinal Chemistry, 16, 8366-8372 (2008).

Li, B., Takegawa, K., Suzuki, T., Yamamoto, K., Wang, L. X., “Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-beta-N-acetylglucosaminidases”, Bioorganic & Medicinal Chemistry, 16, 4670-4675 (2008).

Wang, L. X., “Chemoenzymatic synthesis of glycopeptides and glycoproteins through endoglycosidase-catalyzed transglycosylation”, Carbohydrate Research, 343, 1509-22 (2008).

Umekawa, M., Huang, W., Li, B., Fujita, K., Ashida, H., Wang, L. X.,* Yamamoto, K.,* “Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities”, Journal of Biological Chemistry, 283, 4469-4479 (2008).

Li, X., Wang, L. X., Wang, X., Roseman, S., “The chitin catabolic cascade in the marine bacterium Vibrio Cholerae: Characterization of a unique chitin oligosaccharide deacetylase”. Glycobiology, 17, 1377-1387 (2007).

Li, H. G., Guan, Y., Szczepanska, A., Moreno-Vargas, A. J., Carmona, A. T., Robina, I., Lewis, G. K., Wang, L. X., "Synthesis and anti-HIV activity of trivalent CD4-mimetic miniproteins", Bioorganic & Medicinal Chemistry, 15, 4220-4228 (2007).

Wang, J., Li, H., Zou, G., Wang, L. X., "Novel template-assembled oligosaccharide clusters as epitope mimics for HIV-neutralizing antibody 2G12. Design, synthesis, and antibody binding study", Organic & Biomolecular Chemistry, 5, 1529-1540 (2007).

Wang, L. X., "Toward oligosaccharide- and glycopeptide-based HIV vaccines" Current Opinion in Drug Discovery and Development, 9, 194-206 (2006).

Li, B., Song, H., Hauser, S., Wang, L. X. "A highly efficient chemoenzymatic approach toward glycoprotein synthesis", Organic Letters, 8, 3081-3084 (2006).

Zeng, Y., Wang, J., Li, B., Hauser, S., Li, H., Wang, L. X. "Glycopeptide synthesis through endoglycosidase-catalyzed oligosaccharide transfer of sugar oxazolines. Probing substrate structural requirement." Chemistry. A European Journal, 12, 3355-3364 (2006).

Ni. J., Song, H., Wang, Y., Stamatos, N., Wang, L. X. "Toward a carbohydrate-based HIV-1 vaccine: Synthesis and immunological studies of oligomannose-containing glycoconjugates". Bioconjugate Chemistry, 17, 493-500 (2006).

Li, H, Li, B., Song, H., Breydo, L., Baskakov, I. V., Wang, L. X. "Chemoenzymatic synthesis of HIV-1 V3 domain glycopeptides carrying two N-Glycans and effects of glycosylation on the peptide's domain". Journal of Organic Chemistry, 70, 9990-9996 (2005)

Li, B., Zeng, Y., Hauser, S., Song, H., Wang, L. X. "Highly efficient endoglycosidase-catalyzed synthesis of glycopeptides using oligosaccharide oxazolines as donor substrates". Journal of the American Chemical Society, 127, 9692-9693 (2005).

Wang, J., Le, N., Heredia, A., Song, H., Redfield, R., Wang, L. X. "Modification and structure-activity relationship of a small molecule HIV-1 inhibitor targeting the viral envelope glycoprotein gp120". Organic & Biomolecular Chemistry, 3, 1781-1786 (2005).

Stamatos, N. M., Liang, F., Nan, X., Landry, K., Cross, A. S., Wang, L. X. & Pshezhetsky, A. V. "Endogeneous sialidase activity of human monocytes is up-regulated during cellular differentiation into macrophages". FEBS Journal, 272, 2545-2556 (2005).

Wang, L. X., Song, H., Liu, S., Lu, H., Jiang, S., Ni, J., Li, H. "Chemoenzymatic synthesis of HIV-1 glycopeptides. Effects of glycosylation on the antiviral activity and alpha-helix bundle-forming ability of gp41 peptide C34". ChemBioChem, 6, 1068-1074 (2005).

Hauser, S., Song, H., Li, H., Wang, L. X. "A novel fluorescence-based assay for the transglycosylation activity of endo-beta-N-acetyl-glucosaminidases". Biochemical & Biophysical Research Communications, 328, 580-585 (2005).

Li, H., Singh, S., Song, H., Zeng, Y., Wang, L. X. "Chemoenzymatic synthesis of CD52 glycoprotein carrying native N-glycans". Bioorganic and Medicinal Chemistry Letters, 15, 895-898 (2005).

Wang, L. X., Heredia, A., Song, H., Zhang, Z., Yu, B., Davis, C. & Redfield, R. "Resveratrol glucuronides as the metabolites of resveratrol in humans: characterization, synthesis, and anti-HIV activities". Journal of Pharmaceutical Sciences, 93, 2448-2457 (2004).

Li, H., Song, H., Heredia, A., Le, N., Redfield, R., Lewis, G. K., & Wang, L. X. "Synthetic bivalent CD4-mimetic miniproteins show enhanced anti-HIV activity over the monovalent miniprotein". Bioconjugate Chemistry, 15, 783-789 (2004).

Ni, J., Powell, R., Baskakov, I. V., DeVico, A., Lewis, G. K. & Wang, L. X. "Synthesis, conformation, and immunogenicity of monosaccharide-centered multivalent HIV-1 gp41 peptides containing the sequence of DP178". Bioorganic & Medicinal Chemistry, 12, 3141-3148 (2004).

Naicker, K. P., Li, H., Heredia, A., Song, H., & Wang, L. X. Design and synthesis of Gal-conjugated peptide T20 as novel antiviral agent for HIV-immunotargeting. Organic & Biomolecular Chemistry, 2, 660-664 (2004).

Wang, L. X., Ni, J., Singh, S. & Li, H. "Binding of high-mannose type oligosaccharides and synthetic oligomannose clusters to human antibody 2G12. Implications for HIV-1 vaccine design". Chemistry & Biology, 11, 127-134 (2004).

Li, H. & Wang, L. X. "Design and synthesis of a cholic acid-based oligomannose cluster as the epitope mimic for human HIV-neutralizing antibody 2G12". Organic & Biomolecular Chemistry, 2, 483-488 (2004).




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