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Jian  Zhang
 

Jian Zhang Ph.D.

Academic Title: Assistant Professor
Primary Appointment: Biochemistry and Molecular Biology
jzhang@som.umaryland.edu
Location: IHV, N265
Phone: (410) 706-7500

Personal History:

Dr. Zhang received his Bachelor degree from Beijing Normal University of China, Master of Science from Institute of Chemistry Chinese Academy of Sciences, and PhD from Ibaraki University of Japan. He completed his postdoctoral researches at North Carolina State University with Dr. Marye Anne Fox and University of North Carolina at Chapel Hill with Dr. Royce W. Murray. In 2003, he joined the Center for Fluorescence Spectroscopy (CFS), University of Maryland School of Medicine as an assistant professor of Biochemistry and Molecular Biology Department.

He published more than 80 peer-reviewed papers, and funded as a principal investigator from the National Institutes of Health.

Research Interests:

Dr. Zhang's research focuses on understanding near-field interaction of fluorophore with metallic nanostructure or other photonic band-gap structure and using this approach to develop new fluorescence techniques for biological and medical applications. The basic idea of his research is using the physical principle and chemical tool for detecting biological target. For the near-field interaction approach, typically, a metal nanoparticle with a subwavelength size can create a local electromagnetic field nearby when irradiated by light. Once a fluorophore is placed in this local field within a near-field distance from the metal nanoparticle, the fluorophore can be regarded as a dipole to couple with the plasmon of metal nanoparticle leading to significant increases in its excitation or/and emission rates. Fluorescence from the fluorophore hence can be significantly enhanced to several to thousand folds. In addition, the near-field interaction can increase the photostability and reduce the photoblinking of fluorophore. On the basis of improved optical properties of fluorophore, novel luminescent metal nanoparticles can be developed to monitor single target molecule activities in cell by fluorescence cell imaging. Highly sensitive bioassays can be developed on the specially designed nanostructure substrates. Multiple-functional nanoparticles are being developed to complete the functions of isolation, identification, and delivery in one-step treatment. These results are being used for HIV- and cancer-associated diagnosis and treatment.


Publications:

  1. Fu, Y.; Zhang, J.; Nowaczyk, K.; Lakowicz J. R. Enhanced single molecule fluorescence and reduced observation volumes on nanoporous gold (NPG) films. Chemical Communication 2013, 48, 9726-9728
  2. Zhang, J.; Fu, Y.; Wang, Y.; Lakowicz, J. R. Luminescent properties of Eu(III) chelates on metal nanorods. Journal of Physical Chemistry C 2013, 117, 9372-9380.
  3. Fu, Y.; Zhang, J.; Joseph, Lakowicz, J. R. Largely enhanced single-molecule fluorescence in plasmonic nanogaps formed by hybrid silver nanostructures Langmuir 2013, 29, 2731-2738.
  4. Zhang, J.; Fu, Y.; Conroy, C. V.; Tang, Z.; Li, G.; Zhao, R. Y.; Wang, G. Fluorescence intensity and lifetime cell imaging with luminescent gold nanoclusters Journal of Physical Chemistry C 2012, 116, 26561-2656.
  5. Fu, Y.; Zhang, J.; Lakowicz, J. R. Photophysical behaviors of single fluorophores localized on zinc oxide nanostructures International. Journal of Molecular Sciences 2012, 13, 12100-12112.
  6. Fu, Y.; Zhang, J.; Lakowicz, J. R. Large enhancement of single molecule fluorescence by coupling to hollow silver nanoshells. Chemical Communications 2012, 48, 9726-9728.
  7. Zhang, J.; Fu, Y.; Mahdavi, F. Bimetallic nanoshells for broad band metal-enhanced fluorescence. Journal of Physical Chemistry C 2012, 116, 24224-24232.
  8. Zhang, J.; Fu, Y.; Xu, X.; Lakowicz, J. R. Target molecule imaging on tissue specimens by fluorescent metal nanoprobe. Journal of Biomedical Optics 2011, 16, 116004.
  9. Zhang, J.; Fu, Y.; Li, G.; Lakowicz, J. R.; Zhao, R. Y. Fluorescent metal nanoshell and CK19 detection on single cell image. Biochemical and Biophysical Research Communications 2011, 413, 53-57.
  10. Zhang, J.; Fu, Y.; Lakowicz, J. R. Fluorescent metal nanoshells: lifetime-tunable molecular probes. Journal of Physical Chemistry C 2011, 115, 7255-7260.
  11. Fu, Y.; Zhang, J.; Lakowicz, J. R. Metallic-nanostructure-enhanced fluorescence of single flavin cofactor and single flavoenzyme molecules. Journal of Physical Chemistry C 2011, 15, 7202-7208.
  12. Zhang, J.; Fu, Y.; Li, G.; Zhao, R. Y.; Lakowicz, J. R. Direct observation of chemokine receptors on T-lymphocyte cell surfaces using fluorescent metal nanoprobes 2: Approximation of CCR5 populations. Biochemical and Biophysical Research Communications 2011, 407, 63-67.
  13. Zhang, J.; Fu, Y.; Li, G.; Zhao, R. Y.; Lakowicz, J. R. Detection of CXCR4 receptors on cell surface using fluorescent metal nanoshell. Journal of Biomedical Optics 2011, 16, 016011.
  14. Zhang, J.; Fu, Y.; Li, G.; Nowaczyk, K.; Zhao, R. Y.; Lakowicz, J. R. Direct observation to chemokine receptor 5 on T-lymphocyte cell surface using fluorescent metal nanoprobes. Biochemical and Biophysical Research Communications 2010, 400, 111-116.
  15. Yu, L.; Todd, N. W.; Xing, L.; Xie, Y.; Zhang, H.; Liu, Z.; Fang, H.; Zhang, J.; Katz, R. L.; Jiang, F. Early detection of lung adenocarcinoma in sputum by a panel of microRNA markers. International Journal of Cancer, 2010, 127, 2870-2878.
  16. Zhang, J.; Fu, Y.; Mei, Y.; Jiang F.; Lakowicz, J. R. Fluorescent metal nanoshell probe to detect single miRNA in lung cancer cell. Analytical Chemistry 2010, 82, 4464-4471.
  17. Zhang, J.; Fu, Y.; Jiang F.; Lakowicz, J. R. Metal nanoshell - capsule for light-driven release of small molecule. Journal of Physical Chemistry C 2010, 114, 7653-7659.
  18. Fu, Y.; Zhang, J.; Lakowicz, J. R. Plasmon-enhanced fluorescence from single fluorophores end-linked to gold nanorods. Journal of the American Chemical Society 2010, 132, 5540-5541.