|
Personal History
M.S.: Biophysics; Univerzita Pavla Jozefa Šafárika, Slovakia
Ph.D.: Membrane physiology, Calcium metabolism; Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Slovak Republic
Postdoctoral: Brain ischemia; Experimental Brain Research Lab, Lund University, Sweden
Research Interests
My research is focused on the fundamental mechanisms leading to neuronal degeneration and brain damage due to acute and chronic neurodegenerative disorders. Our studies are centered on processes leading to mitochondrial dysfunction in the brain during neurological disease. Our research is focused on characterization of different mitochondrial population in brain and the determination of their role in mechanisms leading to brain damage. To examine the role of different mitochondrial proteins in mechanisms leading to cells death we generate transgenic animals where proteins of interest are expressed and targeted to mitochondria or knockdown in cell-type specific manner.
Publications
Kristian T, Bernardi P, Siesjo BK (2001) Acidosis promotes the permeability transition in energized mitochondria: Implications for reperfusion injury. J Neurotrauma,18:1059-1074. Li P-A, Kristian T, Ouyang YB, Siesjo BK: Reperfusion damage in the brain. In Cerebrovascular Disease: Momentum at the End of the Second Millennium, Choi D, Dacey R, Hsu C, Powers W, Eds. New York, Futura Publishing Company, 2001
Yoshimoto T, Kristian T, Hu B-R, Ouyang Y-B, Siesjo BK (2002) Effect of NXY-059 on secondary mitochondrial dysfunction after transient focal ischemia; comparison with cyclosporin A. Brain Res 932: 99-109.
Kristian T, Weatherby TM, Bates TE, Fiskum G (2002) Heterogeneity of the calcium-induced permeability transition in isolated non-synaptic brain mitochondria. J of Neurochem. 83:1207-1308.
Bambrik L. , Kristian T., Fiskum G. (2004) Astrocyte mitochondrial mechanisms of ischemic brain injury and neuroprotection. Neurochem Res 29: 601-609.
Kristian T (2004) Metabolic stages, mitochondria, and calcium in hypoxic/ischemic brain damage. Cell Calcium 36: 221-233.
Kristian T, Fiskum G (2004) A Fluorescence-Based Technique for Screening Compounds that Protect Against Damage to Brain Mitochondria. Brain Res Protocols 13:176-182.
Schuh RA, Kristian T, Fiskum G (2005) Calcium-dependent dephosphorylation of brain mitochondrial calcium/cAMP response element binding protein (CREB). J Neurochem 92(2): 388-394.
Kristian T, Hopkins IB, McKenna MC, Fiskum G (2006). Isolation of mitochondria with high respiratory control from primary cultures of neurons and astrocytes using nitrogen cavitation. J Neurosci Methods 152:136-143.
Chandrasekaran K, Hazelton JL, Wang Y, Fiskum G, Kristian T (2006) Neuron-specific conditional expression of a mitochondrially-targeted fluorescent protein in mice. J Neuroscience, 26:13123-13127.
Mehrabian Z, Chandrasekaran K, Kristian T, Fiskum G, Kalvakolanu DB (2007) The interferon b and retinoioc acid induced cell death regulator GRIM-19 are upregulated during focal cerebral ischemia. J Interferon Cytokine Res. 27(5): 383-92.
Kristian T, Pivovarova NB, Fiskum G, Andrews SB (2007): Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. J Neurochem. 102(4):1346-56.
Soane L, Kahraman S, Kristian T, Fiskum G (2007). Mechanisms of impaired mitochondrial energy metabolism in acute and chronic neurodegenerative disorders. J Neurosci Res. 85(15):3407-3415.