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David A Rasko
 

David A Rasko Ph.D.

Academic Title: Associate Professor
Primary Appointment: Microbiology and Immunology
Additional Title(s): Associate Professor, Institute for Genome Sciences
drasko@som.umaryland.edu
Location: BioPark II, 801 W. Baltimore St., Suite 619
Phone: (410) 706-6774
Fax: (410) 706-1482

Personal History:

  • B.Sc., 1995, Specialization in Microbiology, with Honors, University of Alberta, Canada
  • Ph.D., 1995-2000, Medical Microbiology and Immunology, University of Alberta, Canada (Advisors : Dr. Glen Armstrong and Dr. Diane Taylor)
  • Post-Doctoral Fellow, 2000-2001, University of Maryland School of Medicine, Department of Microbiology and Immunology, Baltimore, Maryland (Mentor : Harry L.T. Mobley)
  • GenBank Biologist, 2001-2003, National Center for Biotechnology Information, Bethesda, Maryland
  • Staff Scientist/Collaborative Investigator, 2003-2005, The Institute for Genomic Research (TIGR), Rockville, Maryland
  • Research Assistant Professor, 2005-2008, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas
  • Assistant Professor, 2008-present, Institute for Genome Sciences at the University of Maryland School of Medicine
  • Associate Professor, 2013-present, Institute for Genome Sciences at the University of Maryland School of Medicine

Research Interests:

My research focuses on utilizing comparative genomics of closely related isolates to examine pathogen evolution, with a focus on plasmids and other mobile elements; identification of new virulence factors and signaling cascades in the hope of developing novel therapeutics; and examining the functional aspects of these novel features using modern molecular biology tools animal models. My work utilizes pathogens such as the Bacillus cereus group, Escherichia coli and Shigella.


Publications:

See all publications in PubMed.

Selected From 84 Publications:

  1. J.W. Sahl and D.A. Rasko. 2012. Analysis of the global transcriptional profiles of enterotoxigenic Escherichia coli (ETEC) isolate E24377A. Infection and Immunity. 80(3):1232-42.
  2. T.H. Hazen, J.W. Sahl, J.C. Redman, C.R. Morris, S.C. Daugherty, M.C. Chibucos, N.A. Sengamalay, C.M. Fraser-Liggett, H. Steinsland, T.S. Whittam, B. Whittam, S.D. Manning, D.A Rasko. 2012. Draft genome sequences of the Diarrheagenic Escherichia coli collection. Journal of Bacteriology. 194(11):3026-7.
  3. J.W. Sahl, M.N. Matalka and D.A. Rasko. 2012. Phylomark: a tool to find conserved, phylogenetic markers from whole-genome alignments.  Applied and Environmental Microbiology. 78(14):4884-92.
  4. S. Koren, M.C. Schatz, B.P. Walenz, J. Martin, J. Howard, G. Ganapathy, Z. Wang, D.A. Rasko, W.R. McCombie, E.D. Jarvis and A.M. Phillippy. Hybrid error correction and de novo assembly of single-molecule sequencing reads. Nature Methods. Published online July 1, 2012. doi: 10.1038/nbt.2280
  5. S.R. SteyertR, J.W. Sahl, C.M. Fraser, L.D. Teel, F. Scheutz and D.A. Rasko. 2012. Comparative genomics and stx phage characterization of LEE-negative Shiga toxin-producing Escherichia coli. Front Cell Infect Microbiol. 2012;2:133.
  6. D.A. Rasko. 2012. Clostridium difficile healthcare-associated epidemics. Nat Genet. 2012 Dec 16;45(1):6-7.
  7. R. Kansal R, D.A. Rasko, J.W. Sahl, G.P. Munson, K. Roy, Q. Luo, A. Sheikh, K.J. Kuhne and J.M. Fleckenstein. 2013. Transcriptional modulation of Enterotoxigenic Escherichia coli virulence genes in response to epithelial cell interactions. Infect Immun. 81(1):259-70.
  8. J.W. Sahl, H. Steinsland, J.C. Redman, S.V. Angiuoli, J.P. Nataro, H. Sommerfelt and D.A. Rasko. 2011. A comparative genomic analysis of diverse clonal types of enterotoxigenic Escherichia coli reveals pathovar-specific conservation. Infection and Immunity. 79(2):950-60.
  9. J.W. Sahl, A.L. Lloyd, J.C. Redman, T.A. Cebula, D.P. Wood, H.L.T. Mobley and D.A. Rasko. 2011. Genomic characterization of asymptomatic Escherichia coli isolated from the neobladder. Microbiology. 157:1088-1102.
  10. D.A. Rasko, P. Worsham, T.G. Abshire, S.T. Stanley, J.D. Bannan, M.R. Wilson, R.J. Langham, R.S. Decker, L. Jiang, A.M. Phillipy, S.L. Salzberg, M. Pop, M.N. VanErt, L.J. Kenefic, P.S. Keim, C.M. Fraser-Liggett, J. Ravel. 2011. Microbial forensic applications of comparative genome analysis: Identification of Bacillus anthracis genetic markers in the Amerithrax investigation. Proceedings of the National Academy of Sciences. 108(12):5027-32.
  11. J.W. Sahl, J.K. Johnson, A.D. Harris, A.M. Phillippy, W.W. Hsiao, K.A. Thom, and D.A. Rasko. 2011. Genomic comparison of multi-drug resistant invasive and colonizing Acinetobacter baumannii isolated from diverse human body sites reveals genomic plasticity. BMC Genomics. 4;12:291.
  12. D.A. Rasko, D.R. Webster, J.W. Sahl, A. Bashir, N. Boisen, F. Scheutz, E.E. Paxinos, R.Sebra, C.-S. Chin, D. Iliopoulos, A. Klammer, P. Peluso, L. Lee, A.O. Kislyuk, J. Bullard, A. Kasarskis, S. Wang, J. Eid, D. Rank, J.C. Redman, S.R. Steyert, J. Frimodt-Møller, C. Struve, A.M. Petersen, K.A. Krogfelt, J.P. Nataro, E.E. Schadt, M.K. Waldor. 2011. Origins of the E. coli strain causing a large outbreak of HUS in Germany. New England Journal of Medicine. 365(8):709-17.
  13. JM Fleckenstein, PR Hardwidge, GP Munson, DA Rasko, H Sommerfelt and H Steinsland. 2010. Molecular mechanisms of enterotoxigenic Escherichia coli infection. Microbes and Infection. 12(2):89-98.
  14. D.A. Rasko and V. Sperandio. 2010. Anti-virulence strategies to combat bacteria-mediated disease. Nature Reviews Drug Discovery. 9(2):117-28.
  15. K.A. Thom, W.W. Hsiao, A.D. Harris, O.C. Stine, D.A. Rasko and J.K. Johnson. 2010. Patients with Acinetobacter baumannii bloodstream infections are colonized in the gastrointestinal tract with identical strains. American Journal of Infection Control. 38(9):751-3.
  16. J. Ravel, L. Jiang, S.T. Stanley, M.R. Wilson, R.S. Decker, T.D. Read, P. Worhsam, P.S. Keim, S.L. Salzberg, C.M. Fraser-Liggett and D.A. Rasko. 2009. The complete genome sequence of Bacillus anthracis Ames “Ancestor”. Journal of Bacteriology. 191(1):445-6.
  17. N.C. Reading, D.A. Rasko, A.G. Torres, V. Sperandio. 2009. The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesis. Proceedings of the National Academy of Sciences. 106(14):5889-94
  18. D.A. Rasko, M.J. Rosovitz, G.S.A. Myers, E.F. Mongodin, W.F. Fricke, P. Gajer, J. Crabtree, V. Sperandio and J. Ravel. 2008. The pan-genome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates. Journal of Bacteriology. 2008 Oct;190(20):6881-93.
  19. D.A. Rasko, C.G. Moreira, D.R. Li, N.C. Reading, J.M. Ritchie, M.K. Waldor, N. Williams, R. Taussig, S. Wei, M. Roth, D.T. Hughes, J.F. Huntley, M.W. Fina, J.R. Falck and V. Sperandio. 2008. Targeting QseC signaling and virulence for antibiotic development. Science. 2008 Aug 22;321(5892):1078-80.
  20. J.F. Huntley, P.G. Conley, D.A. Rasko, K.E. Hagman, M.A. Apicella, M.V. Norgard. 2008. Native outer membrane proteins protect mice against pulmonary challenge with virulent Type A Francisella tularensis. Infection and Immunity 2008 Aug;76(8):3664-71.
  21. M.M. Kendall*, D.A. Rasko* and V. Sperandio. 2007. Global effects of the cell-to-cell signaling molecules autoinducer-2, autoinducer-3, and epinephrine in a luxS mutant of enterohemorrhagic Escherichia coli. Infection and Immunity. 75(10):4875-84. (* Equal contribution to the study)
  22. D.A. Rasko*, C.D. Esteban* and V. Sperandio. 2007. Development of novel plasmid vectors and a promoter trap system in Francisella tularensis compatible with the pFLN10 based plasmids. Plasmid. 58(2):159-66 (* Equal contribution to the study)
  23. M. Eppinger, M.J. Rosovitz, W.F. Fricke, D.A. Rasko, G. Korkorina, M.-L. Fayolle, L.E. Linder, E. Carniel and J. Ravel. 2007. Genome sequence of Yersinia pseudotuberculosis IP31758, the causative agent of Far East Scarlet Like Fever (FESLF): Linking genome plasticity to pathogenicity. PLOS Genetics. 3(8):e142.
  24. A.L. Lloyd, D.A. Rasko and H.L.T. Mobley. 2007. Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli. Journal of Bacteriology. 189:2532-46.
  25. T.J. Welch, W.F. Fricke, P. McDermott, D. White, M.-L. Rosso, D.A. Rasko, M.K. Mammel, M. Eppinger, M.J. Rosovitz, D. Wagner, L. Rahalison, J.E. LeClerc, J.M. Hinshaw, L.E. Linder, T.A. Cebula, E. Carniel and J. Ravel. 2007. Multiple antimicrobial resistance in plague: PLOS One. 2:e309.
  26. D.A. Rasko, M.J. Rosovitz, O.A. Okstad, D.E. Fouts, L. Jiang, R.Z. Cer, A.B. Kolsta¸, S.R. Gill, and J. Ravel. 2007. Complete sequence analysis of novel plasmids from emetic and periodontal Bacillus cereus isolates reveals a common evolutionary history among the B. cereus group plasmids including B. anthracis pXO1. Journal of Bacteriology. 189:52-64.
  27. G.S.A. Myers, D.A. Rasko, J.K. Cheung, J. Ravel, R. Seshadri, R.T. DeBoy, Q. Ren, J. Varga, M.M. Awad, L.M. Brinkac, S.C. Daugherty, D.H. Haft, R.J. Dodson, R. Madupu, W.C. Nelson, M.J. Rosovitz, S.A. Sullivan, H. Khouri, G.I. Dimitrov, K.L. Watkins, S. Mulligan, J. Benton, D. Radune, D.J. Fisher, H.S. Atkins, T. Hiscox, B.H. Jost, S. J. Billington, J.G. Songer, B.A. McClane, R.W. Titball, J.I. Rood, S.B. Melville, and I.T. Paulsen. 2006. Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens. Genome Research. 16(8):1031-40.
  28. D.A. Rasko, G.S. Myers, J. Ravel. 2005. Visualization of comparative genomic analyses by BLAST score ratio. BMC Bioinformatics. 6:2
  29. D.A. Rasko, M. R. Altherr, C. S. Han and J. Ravel. 2005. Genomics of the Bacillus cereus group of organisms. FEMS Microbiology Reviews. 29(2):303-329.
  30. A.R. Hoffmaster*, J. Ravel*, D.A. Rasko*, G.D. Chapman, M. Chute, C.K. Marston, B.K. De, C.T. Sacchi, C. Fitzgerald, L.W. Mayer, M.C.J. Maiden, F.G. Priest, M. Barker, L. Jiang, R.Z. Cer, J. Rilstone, S.N. Peterson, R.S. Weyant, D.R. Galloway, T.D. Read, T. Popovic and C.M. Fraser. 2004. Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax. Proceedings of the National Academy of Sciences. 101(22): 8449-8454 (*Equal contributions to the publication)
  31. D.A. Rasko, J. Ravel, O.A. Okstad, E. Helgasson, N. Tourasse, R.Z. Cer, L. Jiang, K.A. Shores, D. Fouts, S.V. Angiuoli, J.F. Kolonay, W.C. Nelson, A.B. Kolsto¸, C.M. Fraser, T.D. Read. 2004. The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1. Nucleic Acids Research. 32(3):977-988