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Personal History
Dr. Rao N. Jaladanki, born in India, completed his Master’s degree and earned Ph.D degree in Biology at Sri Venkateswara University, Tirupati, Andhra Pradesh, India in 1992. His postdoctoral fellowship was awarded by the Rockefeller Foundation in 1993 and worked as a Postdoctoral Fellow at Southern Illinois University, Carbondale, IL (Dr. Andrzej Bartke’s lab), and in 1995 he moved to University of Maryland School of Medicine, Baltimore (Dr. Feng’s lab). In 1997, Dr. Jaladanki started his career as a Research Associate with Dr. Jian-Ying Wang, Department of Surgery. In 2004, he was promoted to the position of Assistant Professor of Surgery.
Research Interests
• Gastrointestinal (GI) Mucosal Homeostasis
• Cellular and molecular mechanisms of GI mucosal healing
• Cellular signaling mechanisms in the maintenance of gut epithelial integrity
• Epithelial cell renewal, growth arrest, and apoptosis
microRNAs
• RNA binding proteins and post-transcriptional regulation during GI epithelial cell growth
• TRPC/STIM1/Caveolin involvement during Ca2+-mediated mucosal restitution
• Cytoskeleton and intercellular junctions in epithelial cells
Lab Techniques and Equipment
• Mucosal injury models in vivo as well as in vitro;
• Epithelial restitution in vitro;
• Measurements of paracellular permeability and barrier functions;
• Nuclear run-on transcription assays and gel shift assays;
• RNA-pull down assays;
• Polysome profile analysis;
• Transient (adenoviral system) and stable gene transfection;
• Promoter deletion, exchange, and point mutations and reporter gene assays;
• Fluorescence measurement of intracellular Ca2+ concentration
• miRNA profiles and RNA Binding Proteins
Grant Support:
Department of Veteran Affairs Merit Review Program
Publications
Recent Publications (Articles published as Jaladanki N. Rao)
Keledjian K, Marasa BS, Wang J-Y, and Rao JN. Induced PDK1 kinase activity suppresses apoptosis in intestinal epithelial cells by activating Akt signaling following polyamine depletion. Int J Clin Exp Med 2012 (In Press).
Timmons J, Chang ET, Wang J-Y and Rao JN. Polyamines and gut mucosal homeostasis. J Gastrointest Digest Sys S7:001, 2012.
Rao JN, Rathor N, Zhuang R, Zou T, Liu L, Xiao L, Turner DJ, and Wang J-Y. Polyamines regulate intestinal epithelial restitution through TRPC1-medidated Ca2+ signaling by differentially modulating STIM1 and STIM2. Am J Physiol Cell Physiol 2012 (In Press).
Zou T, Rao JN, Liu L, Xiao L, Cui YH, Jiang Z, Ouyang M, Donahue JM, and Wang J-Y. Polyamines inhibit the assembly of stress granules in normal intestinal epithelial cells regulating apoptosis. Am J Physiol Cell Physiol, 2012 (In press).
Liu L, Rao JN, Zou T, Xiao L, Smith A, Zhuang R, Turner DJ, and Wang J-Y. Activation of Wnt3a signaling stimulates intestinal epithelial repair by promoting c-Myc-regulated gene expression. Am J Physiol Cell Physiol, 302: C277-C285, 2012.
Cui YH, Xiao L, Rao JN, Zou T, Liu L, Chen Y, Turner DJ, Gorospe M and Wang J-Y. miR-503 represses CUGBP1 translation by recruiting CUGBP1 mRNA to P-bodies. Mol Biol Cell 23: 151-162, 2012.
Rao JN and Wang J-Y. Regulation of Gastrointestinal Mucosal Growth. Text book-ebook Series. Integrated Systems Physiology: From Molecule to function to disease (eds) Neil D. Granger and Joey P. Granger, Morgan & Claypool Life sciences, pp.1-103, 2011.
Xiao L, Cui YH, Rao JN, Zou T, Liu L, Smith A, Turner DJ, Gorospe M and Wang J-Y. Regulation of Cyclin-Dependent Kinase 4 Translation through CUG-Binding Protein 1 and microRNA-222 by Polyamines. Mol Biol Cell 22: 3055-3069, 2011.
Yu T, Wang PY, Rao JN, Zou T Liu L, Xiao L, Gorospe M and Wang J-Y. ChK2-dependent HuR phosphorylation regulates occludin mRNA translation and epithelial barrier function. Nucleic Acids Res 39: 8472-8487, 2011.
Rathor N, Wang SR, Chang ET, and Rao JN. Differentiated intestinal epithelial cells express high levels of TGF-Beta receptors and exhibit increased sensitivity to growth inhibition. Int J Clin Exp Med 4: 299-308, 2011.
Rao JN, Rathor N, Zou T, Liu L, Xiao L, Yu TX, Cui YH, and Wang J-Y. STIM1 translocation to the plasma membrane enhances intestinal epithelial restitution by inducing TRPC1-medidated Ca2+ signaling after wounding. Am J Physiol Cell Physiol 299: C579-C588, 2010.
Zou T, Rao JN, Liu L, Xiao L, Yu TX, Ping J, Gorospe M, and Wang J-Y. Polyamines regulate the stability of JunD mRNA by modulating the competitive binding of its 3’-untranslated region to HuR and AUF1. Mol Cell Biol 30: 5021-5032, 2010.
Rao JN, Liu SV, Zou T, Liu L, Xiao L, Zhang X, Bellavance E, Yuan J, X-J and Wang J-Y. Rac1 promotes intestinal epithelial restitution by increasing Ca2+ influx through interaction with Phospholipase C-¿1 after wounding. Am J Physiol Cell Physiol, 295: C1499-C1509, 2008.
Rao JN, Liu L, Zou T, Marasa BS, Boneva D, Wang SR, Malone DL, Turner DJ and Wang J-Y. Polyamines are required for phospholipase-C¿1 expression promoting intestinal epithelial restitution after wounding. Am J Physiol Gastrointest Liver Physiol 292: G335-G343, 2007.
Rao JN, Platoshyn O, Golovina VA, Liu L, Zou T, Marasa BS, Turner DJ, Yuan J. X-J, and Wang J-Y. TRPC1 functions as store-operated Ca2+ channels in intestinal epithelial cells and regulates early mucosal restitution after wounding. Am J Physiol Gastrointest Liver Physiol 290:G782-G792, 2006.