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Rao N. Jaladanki

Rao N. Jaladanki Ph.D.

Academic Title: Associate Professor
Primary Appointment: Surgery
Location: VAMC, 10 N. Greene St., 3C-124
Phone: (410) 605-7808
Fax: (410) 605-7949

Personal History:

Dr. Rao N. Jaladanki, born in India, completed his Ph.D degree in Zoology (Endocrinology) at Sri Venkateswara University, Tirupati, AP, India in 1992. His postdoctoral fellowship was awarded by the Rockefeller Foundation in 1993, worked as a Postdoctoral Fellow at Southern Illinois University, Carbondale, IL (Dr. Andrzej Bartke’s lab), and in 1995 he moved to University of Maryland School of Medicine, Baltimore (Dr. Feng’s lab). In 1997, Dr. Jaladanki started his faculty position as a Research Associate with Dr. Jian-Ying Wang, Department of Surgery.  In 2015, he was promoted to the Associate Professor of Surgery. Since 2010, he is also affiliated with Department of Veterans Affairs, Biomedical Laboratory Research and Development program as a "Research Health Scientist".

Research Interests:

  • Gastrointestinal (GI) Mucosal Homeostasis
  • Cellular and molecular mechanisms of GI mucosal healing
  • Cellular signaling mechanisms in the maintenance of gut epithelial integrity
  • Epithelial cell renewal, growth arrest, and apoptosis
  • microRNAs, Lnc RNAs, RNA binding proteins and post-transcriptional regulation during GI epithelial cell growth and differentiation
  • TRPC1/STIM1/Caveolin involvement during Ca2+-mediated mucosal restitution
  • Cytoskeleton and intercellular junctions in epithelial cells

Lab Techniques and Equipment:

  • Gut mucosal injury models in vitro and in vivo
  • Small GTP binding proteins, GPCRs during wound healing
  • Measurements of paracellular permeability and barrier functions
  • microRNAs regulation on gut mucosal integrity
  • RNA-pull down assays
  • Polysome profile analysis
  • Transient (adenoviral system) and stable gene transfection
  • Promoter deletion, exchange, and point mutations and reporter gene assays
  • Fluorescence measurement of intracellular Ca2+ concentration
  • miRNA profiles and RNA Binding Proteins

Grant Support:

Department of Veteran Affairs Merit Review Award


(Selected from 95 articles published as Jaladanki N. Rao)

  1. Phatak P, Byrnes K, Mansour D, Liu L, Li R, Rao JN, Turner DJ, Wang J-Y and Donahue JM. Overexpression of miR-214-3p in esophageal squamous cancer cells enhances sensitivity to cisplatin by targeting survivin directly and indirectly through CUG-BP1. Oncogene 2015 (In Press).
  2. Zou T, Rao JN, Liu L, Xiao L, Chung HK, Li Y, Chen G, Gorospe M, and Wang J-Y. JunD enhances miR-29b levels transcriptionally and post-transcriptionally to inhibit proliferation of intestinal epithelial cells. Am J Physiol Cell Physiol308: C813-C824, 2015.
  3. Rathor N, Zhuang R, Wang JY, Donahue JM, Turner DJ and Rao JN. Src-mediated Caveolin-1 phosphorylation regulates intestinal epithelial restitution by altering Ca2+ influx after wounding. Am J Physiol Gastrointest & Liver Physiol  306: G650-G658, 2014.
  4. Rathor N, Chung HK, Wang SR, Wang J-Y, Turner DJ and Rao JN. Caveolin-1 enhances rapid mucosal restitution by activating TRPC1-mediated Ca2+ signaling.  Physiol Rep  2: e12193, 2014.
  5. Zhuang R, Rao JN, Zou T, Liu L, Xiao L, Cao S, Hansraj NZ, Gorospe M, Wang JY. miR-195 competes with HuR to modulate stim1 mRNA stability and regulate cell migration. Nucleic Acids Res 41: 7905-7919, 2013.
  6. Yu T, Rao JN, Zou T, Liu L, Xiao L, Ouyang M, Cao S, Gorospe M and Wang JY. Competitive binding of CUGBP1 and HuR to Occludin mRNA controls its translation and modulates epithelial barrier function. Mol Biol Cell 24: 85-99, 2013.
  7. Keledjian KM, Marasa BS, Wang JY, and Rao JN. Induced PDK1 kinase activity suppresses apoptosis in intestinal epithelial cells by activating Akt signaling following polyamine depletion. Int J Clin Exp Med 5: 221-228, 2012.
  8. Timmons J, Chang ET, Wang J-Y and Rao JN. Polyamines and gut mucosal homeostasis. J Gastrointest Digest Sys S7:001, 2012.
  9. Rao JN, Rathor N, Zhuang R, Zou T, Liu L, Xiao L, Turner DJ, and Wang JY. Polyamines regulate intestinal epithelial restitution through TRPC1-mediated Ca2+ signaling by differentially modulating STIM1 and STIM2. Am J Physiol Cell Physiol 303: C308-C317, 2012.
  10. Rathor N, Wang SR, Chang ET, and Rao JN. Differentiated intestinal epithelial cells express high levels of TGF-b receptors and exhibit increased sensitivity to growth inhibition. Int J Clin Exp Med 4: 299-308, 2011.
  11. Rao JN and Wang JY. Regulation of Gastrointestinal Mucosal Growth. Text book-ebook Series. Integrated Systems Physiology: From Molecule to function to disease#15 (eds) Neil D. Granger and Joey P. Granger, Morgan & Claypool Life sciences pp.1-103, 2011.
  12. Rao JN, Rathor N, Zou T, Liu L, Xiao L, Yu TX, Cui YH, and Wang JY. STIM1 translocation to the plasma membrane enhances intestinal epithelial restitution by inducing TRPC1-medidated Ca2+ signaling after wounding. Am J Physiol Cell Physiol 299: C579-C588, 2010.
  13. Zou T, Rao JN, Liu L, Xiao L, Yu TX, Jiang P, Gorospe M, and Wang JY. Polyamines regulate the stability of JunD mRNA by modulating the competitive binding of its 3’-untranslated region to HuR and AUF1. Mol Cell Biol 30: 5021-5032, 2010.
  14. Rao JN, Liu SV, Zou T, Liu L, Xiao L, Zhang X, Bellavance E, Yuan JXJ. and Wang JY. Rac1 promotes intestinal epithelial restitution by increasing Ca2+ influx through interaction with Phospholipase C-g1 after wounding. Am J Physiol Cell Physiol 295: C1499-C1509, 2008.
  15. Rao JN, Liu L, Zou T, Marasa BS, Boneva D, Wang SR, Malone DL, Turner DJ, and Wang JY. Polyamines are required for phospholipase-Cg1 expression promoting intestinal epithelial restitution after wounding. Am J Physiol Gastrointest & Liver Physiol 292: G335-G343, 2007.