Dr. Rao N. Jaladanki, born in India, completed his Master’s degree and earned Ph.D degree in Biology at Sri Venkateswara University, Tirupati, Andhra Pradesh, India in 1992. His postdoctoral fellowship was awarded by the Rockefeller Foundation in 1993 and worked as a Postdoctoral Fellow at Southern Illinois University, Carbondale, IL (Dr. Andrzej Bartke’s lab), and in 1995 he moved to University of Maryland School of Medicine, Baltimore (Dr. Feng’s lab). In 1997, Dr. Jaladanki started his career as a Research Associate with Dr. Jian-Ying Wang, Department of Surgery. In 2004, he was promoted to the position of Assistant Professor of Surgery.
- Gastrointestinal (GI) Mucosal Homeostasis
- Cellular and molecular mechanisms of GI mucosal healing
- Cellular signaling mechanisms in the maintenance of gut epithelial integrity
- Epithelial cell renewal, growth arrest, and apoptosis microRNAs
- RNA binding proteins and post-transcriptional regulation during GI epithelial cell growth
- TRPC/STIM1/Caveolin involvement during Ca2+-mediated mucosal restitution
- Cytoskeleton and intercellular junctions in epithelial cells
Lab Techniques and Equipment:
- Mucosal injury models in vivo as well as in vitro;
- Epithelial restitution in vitro;
- Measurements of paracellular permeability and barrier functions;
- Nuclear run-on transcription assays and gel shift assays;
- RNA-pull down assays;
- Polysome profile analysis;
- Transient (adenoviral system) and stable gene transfection;
- Promoter deletion, exchange, and point mutations and reporter gene assays;
- Fluorescence measurement of intracellular Ca2+ concentration
- miRNA profiles and RNA Binding Proteins
Department of Veteran Affairs Merit Review Program
(Selected from 85 articles published as Jaladanki N. Rao)
- Rathor N, Zhuang R, Wang JY, Donahue JM, Turner DJ and Rao JN. Src-mediated Caveolin-1 phosphorylation regulates intestinal epithelial restitution by altering Ca2+ influx after wounding. Am J Physiol Gastrointest & Liver Physiol 306: G650-G658, 2014.
- Yang H, Rao JN, and Wang J-Y. Posttranscriptional regulation of tight junctions and epithelial barrier function by RNA-binding proteins and microRNAs. Tissue Barriers 2:e28320, 2014.
- Rao JN. Estrogens and Gastroparesis: A Clinical Relevance. Dig Dis Sci, 6: 1449-1451, 2013.
- Zhuang R, Rao JN, Zou T, Liu L, Xiao L, Cao S, Hansraj NZ, Gorospe M, Wang JY. miR-195 competes with HuR to modulate stim1 mRNA stability and regulate cell migration. Nucleic Acids Res 41: 7905-7919, 2013.
- Yu T, Rao JN, Zou T, Liu L, Xiao L, Ouyang M, Cao S, Gorospe M and Wang JY. Competitive binding of CUGBP1 and HuR to Occludin mRNA controls its translation and modulates epithelial barrier function. Mol Biol Cell 24: 85-99, 2013.
- Xiao L, Rao JN, Zou T, Liu L, Cao S, Martindale JL, Su W, Chung HK, Gorospe M and Wang J-Y. miR-29b Represses Intestinal Mucosal Growth by Inhibiting Translation of Cyclin-dependent Kinase 2. Mol Biol Cell, 24: 3038-3046, 2013.
- Keledjian KM, Marasa BS, Wang JY, and Rao JN. Induced PDK1 kinase activity suppresses apoptosis in intestinal epithelial cells by activating Akt signaling following polyamine depletion. Int J Clin Exp Med 5: 221-228, 2012.
- Timmons J, Chang ET, Wang J-Y and Rao JN. Polyamines and gut mucosal homeostasis. J Gastrointest Digest Sys S7:001, 2012.
- Rao JN, Rathor N, Zhuang R, Zou T, Liu L, Xiao L, Turner DJ, and Wang JY. Polyamines regulate intestinal epithelial restitution through TRPC1-mediated Ca2+ signaling by differentially modulating STIM1 and STIM2. Am J Physiol Cell Physiol 303: C308-C317, 2012.
- Rathor N, Wang SR, Chang ET, and Rao JN. Differentiated intestinal epithelial cells express high levels of TGF-b receptors and exhibit increased sensitivity to growth inhibition. Int J Clin Exp Med 4: 299-308, 2011.
- Rao JN and Wang JY. Regulation of Gastrointestinal Mucosal Growth. Text book-ebook Series. Integrated Systems Physiology: From Molecule to function to disease#15 (eds) Neil D. Granger and Joey P. Granger, Morgan & Claypool Life sciences pp.1-103, 2011.
- Rao JN, Rathor N, Zou T, Liu L, Xiao L, Yu TX, Cui YH, and Wang JY. STIM1 translocation to the plasma membrane enhances intestinal epithelial restitution by inducing TRPC1-medidated Ca2+ signaling after wounding. Am J Physiol Cell Physiol 299: C579-C588, 2010.
- Zou T, Rao JN, Liu L, Xiao L, Yu TX, Jiang P, Gorospe M, and Wang JY. Polyamines regulate the stability of JunD mRNA by modulating the competitive binding of its 3’-untranslated region to HuR and AUF1. Mol Cell Biol 30: 5021-5032, 2010.
- Rao JN, Liu SV, Zou T, Liu L, Xiao L, Zhang X, Bellavance E, Yuan JXJ. and Wang JY. Rac1 promotes intestinal epithelial restitution by increasing Ca2+ influx through interaction with Phospholipase C-g1 after wounding. Am J Physiol Cell Physiol 295: C1499-C1509, 2008.
- Rao JN, Liu L, Zou T, Marasa BS, Boneva D, Wang SR, Malone DL, Turner DJ, and Wang JY. Polyamines are required for phospholipase-Cg1 expression promoting intestinal epithelial restitution after wounding. Am J Physiol Gastrointest & Liver Physiol 292: G335-G343, 2007.
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