Research Interests:The principle area of my research has been the study of protozoan parasites and how they interact with their respective hosts. Currently, I have research projects underway to investigate several aspects of the liver stage of Plasmodial parasites. The liver has been demonstrated to be a target for the host's immune response, yet the exact antigenic targets have not been clearly defined. I am interested in identifying antigens that may be respon-sible for the induction of a protective immune response. Additionally, the mechanisms that are utilized to protect the host are being studied. Both Plasmodium yoelii and Plasmodium falciparum are being used to study these molecular and immunological events that accompany infection of hepatocytes.
Gaywee J., J.B. Sacci Jr., S. Radulovic, M.S. Beier, A.F. Azad. 2003. Subcellular localization of rickettsial invasion protein, InvA. Am. J. Trop. Med. Hyg. 68:92-96.
Sacci Jr., J.B., A.F. Azad. 2002. Gene expression analysis during liver stage development of Plasmodium. Int. J. Parasitol. 32(13):1551-1557.
Florens, L., M.P. Washburn, J.D. Raine, R.M. Anthony, M. Grainger, J.D. Haynes, J.K. Moch, N. Muster, J.B. Sacci, D.L. Tabb, A.A.Witney, D. Wolters, Y, Wu, M.J. Gardner, A.A. Holder, R.E. Sinden, J.R. Yates, D.J. Carucci. 2002. A proteomic view of the Plasmodium falciparum life cycle. Nature. 419(6906):520-526.
Sacci Jr., J.B. 2002. Inhibition of liver-stage development assay. Methods Mol. Med. 72:517-20.
Sacci Jr., J.B. 2002. Hepatocyte perfusion, isolation, and culture. Methods Mol. Med. 72:503-505.
Sacci Jr., J.B. 2002. Hepatic portal branch inoculation. Methods Mol. Med. 72:499-501.
Hoffman, S.L., L.M. Goh, T.C. Luke, I. Schneider, T.P. Le, D.L. Doolan, J. Sacci, P. de La Vega, M. Dowler, C. Paul, D.M. Gordon, J.A. Stoute, L.W. Church, M. Sedegah, D.G. Heppner, W.R. Ballou, T.L. Richie. 2002. Protection of Humans against Malaria by Immunization with Radiation-Attenuated Plasmodium falciparum Sporozoites. J. Infect. Dis. 185(8):1155-1164.
Rathore D., J.B. Sacci, P. de la Vega, T.F. McCutchan. 2002. Binding and invasion of liver cells by Plasmodium falciparum sporozoites. Essential involvement of the amino terminus of circumsporozoite protein. J. Biol. Chem. 277(9):7092-7098.
Sacci Jr., J.B., J.C. Aguiar, A.O. Lau, S.L. Hoffman. 2002. Laser capture microdissection and molecular analysis of Plasmodium yoelii liver-stage parasites. Mol. Biochem. Parasitol. 119:285-289.
Kolodny N., S. Kitov, M.A. Vassell, V.L. Miller, L.A. Ware, K. Fegeding, P. De La Vega, J.B. Sacci Jr, D.E. Lanar. 2001. Two-step chromatographic purification of recombinant Plasmodium falciparum circumsporozoite protein from Escherichia coli. J Chromatogr B, 762(1):77-86.
Aguiar, J.C., R.C. Hedstrom, W.O. Rogers, Y. Charoenvit, J. B. Sacci, D.E. Lanar, V.F. Majam, R.R. Stout, S.L. Hoffman. 2001. Enhancement of the immune response in rabbits to a malaria DNA vaccine by immunization with a needle-free jet device. Vaccine 20:275-280.
Joshi, M.B., A.A. Gam, R.A. Boykins, S. Kumar, J. Sacci, S.L. Hoffman, H.L. Nakhasi, R.T. Kenney. 2001. Immunogenicity of well-characterized synthetic Plasmodium falciparum multiple antigen peptide conjugates. Infect. & Immun. 69:4884-4890.
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