Dr. Trisler is a developmental neuroscientist. He has studied molecular mechanisms for proper neuronal wiring and synapse formation during development of the Central Nervous System. He has identified molecules that define topographic maps of cell position in the embryonic visual system during synapse formation. His current research is in regenerative medicine, entailing the use of embryonic neural stem cells and adult haematopoietic stem cells for cell replacement therapy and neuroprotective gene delivery to the CNS in mouse models of Parkinson’s Disease, Multiple Sclerosis and Amyotrophic Lateral Sclerosis (ALS). The Medical School holds patents on his method to grow pure populations of stem cells from adult human, mouse and rat bone marrow and the use of those stem cells for replacement therapy and gene delivery. Stem cells derived by his method were found to become cells with the morphology and molecular phenotype of neurons, astrocytes and oligodendrocytes when transplanted into adult mouse brain. Stem cells grown from adult mouse bone marrow and engineered to express either Interferon-ß or Brain Derived Neurotrophic Factor were found to improve clinical scores of Experimental Autoimmune Encephalomyelitis mice, a mouse model of Multiple Sclerosis. The goal of his research is to use a patient’s own stem cells for cell replacement and gene therapy.
Goolsby, J., Marty, M.., Heletz, D., Chiappeli, J., Tashko, G., Yarnell, D., Fishman, P., Dhib-Jalbut, S., Bever, C., Pessac, B. & Trisler, D. 2003. Hematopoietic progenitors express neural genes. Proc. Natl. Acad. Sci., USA 100: 14926-14931.
Makar, T., Trisler, D., Eglitis, M., Mouradian, M. & Dhib-Jalbut, S. 2004. Brain-derived neurotrophic factor (BDNF) gene delivery into the CNS using bone marrow cells as vehicles in mice. Neurosci. Letts. 356: 215-219.