Ph.D., University of Toronto, 2006
Our research is focused on mechanisms of signaling transduction and transcriptional regulation by ubiquitin ligase and histone demethylase in prostate cancer. We use a variety of approaches including genetic mouse models, traditional cell biology and biochemical techniques to establish the importance of ubiquitin ligase Siah2 and histone demethylase JMJD1A in the development, progression and therapeutic resistance of prostate cancer. The significance of our findings in cell culture and mouse models is routinely verified by analyzing tissue samples from patients with prostate cancer. We hope these basic research will allow us to design better approaches for treatment of advanced prostate cancer in the future.
Lab Techniques and Equipment:
Cell culture, transfection, lentiviral transduction, PCR cloning and mutagenesis, profiling array, real-time PCR, western blotting, immunoprecipitation, luciferase reporter assay, ChIP assay, transgenic prostate cancer model, orthotopic prostate tumor model, immunohistochemistry, confocal microscopy.