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Dan  H Schulze
 

Dan H Schulze Ph.D.

Academic Title: Associate Professor
Primary Appointment: Microbiology and Immunology
Secondary Appointments: Orthopaedics, Otorhinolaryngology-Head & Neck Surgery, Veterinary
dschulze@umnet.umaryland.edu
Location: Bio Park 3-031
Phone: (410) 706-3813
Phone: (410) 706-5180
Fax: (410) 706-2129

Research Interests:

My laboratory has developed an interest in molecular characterization of membrane transport molecules that regulate calcium (Ca) in cells.   Specifically, we initially studied the sodium/calcium (Na/Ca) exchanger in mammalian species, and more recently molecules related to the Na/Ca exchanger proteins in Drosophila and bacteria. We initially discovered that alternative splicing for the Na/Ca exchanger RNA produces tissue-specific exchanger proteins that differ in the regulation of function in various cell types. We are studying the mechanism that provides specificity for RNA splicing and we are also studying how these alternatively spliced forms of the protein alter NCX function in expression systems. We additionally demonstrated that NCX1 is regulated by protein kinase A and that the NCX1 transporter is part of a macromolecular signaling complex in cardiomyocytes. We are currently studying the components of the signaling complex. We are also studying the NCX complex in other tissues. Additionally, the laboratory is interested in the evolutionary aspects of the Na/Ca exchanger and related Ca ion transporters. Study of different transport molecules for this gene family will provide insight into structure and function of ion transporters in general.

Publications:

Schulze DH, Mughal M, Lederer WJ, Ruknudin AM. Sodium/calcium exchanger (NCX1) macromolecular complex. J. Biol Chem. 278:28549-28555, 2003.

Wei, S.K., A. Ruknudin, S.U. Hanlon, J.M. McCurley, D.H. Schulze, and M.C Haigney. Protein Kinase A Hyperphosphorylation Increases Basal Current but Decreases {beta}-Adrenergic Responsiveness of the Sarcolemmal Na+-Ca2+ Exchanger in Failing Pig Myocytes. Circ. Res 92(8):897-903 2003.

Schulze, D.H., S.K. Polumuri, T. Gille and A. Ruknudin. Functional Regulation of Alternatively Spliced Na+/Ca2+ Exchanger (NCX1) Alternatively Spliced Isofoms. Ann. NY Acad. Sci. 976:187-196, 2002.

Polumuri, S.K., A.M. Ruknudin and D.H. Schulze. RNaseH and its effects on PCR. BioTechniques 32:1224-1225, 2002.

Schulze, D.H., S.K. Polumuri, T. Gille and A.M. Ruknudin. Functional regulation of alternatively spliced Na+/Ca2+ exchanger (NCX1) isoforms. NY Acad. Sci.976:187-196, 2002.

Polumuri, S.K., A.M. Ruknudin, M. M. McCarthy, T.S. Perrot-Sinal and D.H. Schulze. Determining the relative levels of transcripts for the multigene family. NY Acad. Sci. 976:60-63, 2002.

Ruknudin, A., S. He, W.J. Lederer and D. H. Schulze. Functional differences between cardiac and renal isoforms of the Na+/Ca2+ exchanger, NCX1. J. Physiology 529:599-610, 2000.

He, S., A. Ruknudin, L.L. Bambrick, W.J. Lederer and D.H. Schulze. Isoform specific regulation of the Na+/Ca2+ exchanger in rat astrocytes and neurons by PKA. J. Neurosci. 18:4833-4841, 1998.