I completed by medical and PhD training at Johns Hopkins University School of Medicine in 2002. I then completed my internal medicine residency training at Johns Hopkins Bayview Medical Center followed by Rheumatology Fellowship at Johns Hopkins, which ended in 2009. From 2009 to 2012 I was an IRTA post-doctoral fellow in the lab of Dr. Ethan Shevach in the Laboratory of Immunology at the NIAID, NIH. During that time I served for one year as a post-doc member of the NIH Immunology Interest Group. Currently, I am a faculty member in the Department of Medicine, Division of Rheumatology and Clinical Immunology. I have a joint appointment at the Baltimore VA, where I see patients in the VA arthritis clinic.
I completed my PhD (Johns Hopkins Graduate Program in Cellular and Molecular Medicine) under the mentorship of Dr. Mark Schlissel. My thesis forcused on the regulation of V(D)J rearrangement by chromatin accessibility. We showed that the basic structure of chromain, the nucleosome, can inhibit cutting of the recombination signal sequence by the RAG1/2 proteins. During my rheumatology fellowship, I switched my focus to the study of human regulatory T cells. I studied the affects of an inflammatory environment in systemic lupus erythemaosus (SLE) on Tregs and showed that Type I IFN can suppress normal Treg expansion. I then pursued additional training in Treg biology in the lab of Dr. Ethan Shevach at the NIAID. I have completed projects looking at the TCR repertoire of human Tregs with deep sequencing of the TCR CDR3 (manuscript in preparation). In addition, I have applied an improved method for identifying human Tregs, which combines Helios with FoxP3, to the study of Tregs in SLE (manuscript submitted). I currently have a 4 year Career Development Award from the VA to study human Tregs in patients with rheumatologic disease. We are hoping to continue to improve our understanding of the role Tregs have in controlling autoimmune disease. In addition, I have a separate project investigation new B-cell derived autoantigens in SLE.
Internal Medicine & Rheumatology (both board-certified)
Lab Techniques and Equipment
Cellular Immunology, Flow Cytometry, Tissue culture differentation of helper T cells, Isolation of lymphocytes with magnetic bead separation, Treg suppression assays, Western Blotting/Protein Biology with patient sera.
Equipment includes an AutoMacsPro from Miltenyi Biotec, standard western blot apparatus, Accuri flow cytometer (property of Dr. Sergei Atamas), and Flow cytometry antibodies; we also have access to rheumatology patient samples through an IRB-approved study in the VA/University of Maryland.
- Golding, A., Weickert, M. J., Tokeson, J. P., Garges, S., and Adhya, S.: A mutation defining ultrainduction of the Escherechia coli gal operon. J. Bacteriol. 173: 6294-6296, 1991.
- Gangi-Peterson, L., Peterson, S. N., Shapiro, L. H., Golding, A., Caricchio, R., Cohen, D. I., Margulies, D., and Cohen, P.: bca: an Activation-related B-cell gene. Molec. Immunol. 35: 55-63, 1998.
- Golding, A., Chandler, S., Ballestar, E., Wolffe, A. P., and Schlissel, M.: Nucleosome structure completely inhibits in vitro cleavage by the V(D)J recombinase. EMBO J. 18: 3712-3723, 1999.
- Cost, G. J., Golding, A., Schlissel, M. S., and Boeke, J. D.: Target DNA chromatinization modulates nicking by L1 endonuclease. Nucleic Acids Res. 29: 573-577, 2001.
- Golding, A., Haque, U. J., and Giles, J. T.: Rheumatoid arthritis and reproduction. Rheumatic Disease Clinics of North America-Pregnancy and Rheumatic Disease. 33: 319-343, 2007.
- Golding, A., Rosen, A., Petri, M., Ahkter, E., and Andrade, F.: Type I interferon regulates the dynamic balance between human effector and regulatory T cells implications for anti-viral and autoimmune responses. Immunology. 131(1):107-17, 2010.
- Golding, A., Illei, G., Hasni, S., and Shevach, E.: The Percentage of Foxp3+Helios+ T Regulatory Cells positively correlates with disease activity in Systemic Lupus Erythematosus; Submitted manuscript, pending review.