Dr. Peiying Yu obtained her degree in Medicine from the University of Beijing Medical University in China. She had excellent research training at the Columbia University and New York State Psychiatric Institute where she was involved in the development and characterization of mouse monoclonal antibodies against serotonin receptors found in rat brain, specifically in the raphe nuclei.
Dr. Yu’s research interest centers on dopamine D1 receptor (D1R) regulation of signal transduction in lipid rafts in renal epithelial cells by elucidating the regulatory mechanisms involved in how these receptors control the oxidant and antioxidant systems to maintain homeostasis. Dr. Yu was first to demonstrate the mechanisms by which the D1R is regulated in LRs in human embryonic kidney (HEK-293) cells heterologously expressing and in rat renal proximal tubule cells (RPTCs). Dr. Yu has intensively studied how the various NAPDH oxidase subunits behave in lipid and non-lipid rafts in RPTCs and described the salient differences in their activity between human and rodent renal proximal tubule cells. She was first to describe that lipid rafts maintain NADPH oxidase in an inactive state in human RPTCs, in contrast to rodent RPTCs where NADPH oxidase is basally active in the lipid rafts. Lipid rafts serve as signaling platforms that spatially concentrate receptors and signaling molecules in order to facilitate and augment their interaction, thereby enhancing the efficiency and specificity of the signal transduction. At present, Dr. Yu is working on several aspects of D1-like dopamine receptor signaling to compare and contrast the mechanisms by which the D1R and D5R differentially regulate the various adenylyl cyclases, protein kinase C (PKC), and small G proteins in lipid vs. non-lipid rafts of human renal epithelial cells.
Dr. Yu has published over 30 peer-reviewed papers, including an invited methods paper on detailing the various protocols for the extraction of lipid rafts from different cell lines. Dr. Yu has expertise on immunohistochemistry, immunofluorescence, and confocal microscopy and remains to be a well-liked mentor to many post-doctoral fellows and new faculty.
Education and Training
- 1965 – 1970: University of Beijing Medical University - B.S. in Medicine
- 1970 – 1978: Anhui Medical University - Resident training
- 1982 – 1983: University of Beijing Medical University - (advanced study)
- 1986 – 1989: Columbia University and New York State Psychiatric Institute
Yu P, Villar VA, Jose PA. Methods for the study of dopamine receptors within lipid rafts of kidney cells. Methods Mol Biol. 2013;964:15-24.
Yu P, Han W, Villar VA, Li H, Arnaldo FB, Concepcion GP, Felder RA, Quinn MT, Jose PA. Dopamine D1 receptor-mediated inhibition of NADPH oxidase activity in human kidney cells occurs via protein kinase A-protein kinase C cross talk. Free Radic Biol Med. 2011 Apr 1;50(7):832-40.
Li H, Yu P, Sun Y, Felder RA, Periasamy A, Jose PA. Actin cytoskeleton-dependent Rab GTPase-regulated angiotensin type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy. J Biomed Opt. 2010 Sep-Oct;15(5):056003.
Li H, Han W, Villar VA, Keever LB, Lu Q, Hopfer U, Quinn MT, Felder RA, Jose PA, Yu P. D1-like receptors regulate NADPH oxidase activity and subunit expression in lipid raft microdomains of renal proximal tubule cells. Hypertension. 2009 Jun;53(6):1054-61.
Li H, Armando I, Yu P, Escano C, Mueller SC, Asico L, Pascua A, Lu Q, Wang X, Villar VA, Jones JE, Wang Z, Periasamy A, Lau YS, Soares-da-Silva P, Creswell K, Guillemette G, Sibley DR, Eisner G, Gildea JJ, Felder RA, Jose PA. Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitin-proteasome pathway in mice and human cells. J Clin Invest. 2008 Jun;118(6):2180-9.
Han W, Li H, Villar VA, Pascua AM, Dajani MI, Wang X, Natarajan A, Quinn MT, Felder RA, Jose PA, Yu P. Lipid rafts keep NADPH oxidase in the inactive state in human renal proximal tubule cells. Hypertension. 2008 Feb;51(2):481-7.
Yu P, Asico LD, Luo Y, Andrews P, Eisner GM, Hopfer U, Felder RA, Jose PA. D1 dopamine receptor hyperphosphorylation in renal proximal tubules in hypertension. Kidney Int. 2006 Sep;70(6):1072-9.
Yang Z, Asico LD, Yu P, Wang Z, Jones JE, Bai RK, Sibley DR, Felder RA, Jose PA. D5 dopamine receptor regulation of phospholipase D. Am J Physiol Heart Circ Physiol. 2005 Jan;288(1):H55-61.
Yu P, Yang Z, Jones JE, Wang Z, Owens SA, Mueller SC, Felder RA, Jose PA. D1 dopamine receptor signaling involves caveolin-2 in HEK-293 cells. Kidney Int. 2004 Dec;66(6):2167-80.
Felder RA, Sanada H, Xu J, Yu PY, Wang Z, Watanabe H, Asico LD, Wang W, Zheng S, Yamaguchi I, Williams SM, Gainer J, Brown NJ, Hazen-Martin D, Wong LJ, Robillard JE, Carey RM, Eisner GM, Jose PA. G protein-coupled receptor kinase 4 gene variants in human essential hypertension. Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3872-7.
Yu P, Asico LD, Eisner GM, Hopfer U, Felder RA, Jose PA. Renal protein phosphatase 2A activity and spontaneous hypertension in rats. Hypertension. 2000 Dec;36(6):1053-8.