|
|
Lindsay W Black
Ph.D.
|
| Academic Title:
Professor |
| Primary Appointment:
Biochemistry and Molecular Biology |
|
lblack@umaryland.edu |
| Location:
108 N. Greene St.,
408 |
| Phone:
(410) 706-3510 |
|
Personal History
Education:
• B.S.- Biochemistry, The University of Chicago (Honors, Phi Beta Kappa)
• Ph.D. - Biochemistry, Department of Biochemistry, Stanford University, School of Medicine
• Post Doctoral - Institute of Molecular Biology, University of Geneva, Switzerland
• Post Doctoral Fellow - Jane Coffin Childs Foundation Swiss National Science Foundation
Professional Experience:
• 1971-1973 - Asst. Prof., Dept. Biol. Chem., School of Medicine, University of Maryland.
• 1974 - Visiting Scientist, Microbiology Dept. Biozentrum Basel University, Basel, Switzerland
• 1973-1982 - Assoc. Prof., Biol. Chemistry, School of Medicine, University of Maryland
• 1983-present - Professor, Dept. of Biochemistry & Mol. Biology, School of Medicine, University of Maryland.
Research Interests
Viral nucleic acid packaging is highly conserved among phages and viruses. Phage assembly by DNA translocation into an empty procapsid is driven by an ATP powered “packasome” motor constituted of a packaging enzyme and a dodecameric portal ring vertex of the procapsid. Recently we have shown that a favored portal rotary motor mechanism does not apply(1). Additionally we provide evidence by efficient packaging of short dye labeled model DNA substrates in a defined two component system evidence for a linear torsional compression motor mechanism (2).
Phage exclusion and anti-exclusion mechanisms: A novel glucose modified restriction gmrS/gmrD enzyme that targets glycosyl-hydroxymethylcytosine modified phage DNAs has been isolated from pathogenic E. coli CT596(3). In response T-even phages have evolved capsid-targeted internal protein enzyme inhibitors injected into the host with the DNA to shield it (4). Analysis of the structures of these inhibitors reveals an evolutionary pathway that has elaborated a surprisingly diverse and specifically fitted set of coevolving attack and defense proteins.
(1) Baumann RG, Mullaney J, Black LW. (2006) Portal fusion protein constraints on function in DNA packaging of bacteriophage T4.Mol Microbiol. 61:16-32.
(2) Sabanayagam C, Oram M, Lakowicz JR, Black LW (2007) Viral DNA packaging studied by fluorescence correlation spectroscopy, Biophysical Journal, 93(4), 17-19.
(3) Bair CL, Black LW (2007). A Type IV modification dependent restriction nuclease that targets glucosylated hydroxymethyl cytosine modified DNAs. J. Mol. Biol. 366, 768.
(4) Bair CL, Rifat D, Black LW (2007). Exclusion of glucosyl-hydroxymethylcytosine DNA containing bacteriophages is overcome by the injected protein inhibitor IPI*. J. Mol. Biol. 366, 779
Laboratory Personnel:
Julienne Mullaney, Research Associate
Mark Oram, Research Associate
Publications
Area 1 of Research
Rao, VB, Black, LW (2005) DNA packaging in bacteriophage T4. in Viral Packaging Machines, (ed. Catalano C), Kluwer Academic/Plenum.
Fokine A, Leiman PG, Shneider MM, Ahvazi B, Boeshans KM, Steven AC, Black LW, Mesyanzhinov VV, and Rossmann MG (2005), Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry. PNAS in press.
Richard G. Baumann and Lindsay W. Black (2003) Isolation and Characterization of T4 Bacteriophage gp17 Terminase, a Large Subunit Multimer with Enhanced ATPase Activity. J. Biol. Chem. 278, 4618-4627.
Black, L.W., (1989). DNA Packaging in dsDNA Bacteriophages. Annu. Rev. Microbiol. 43: 267-292
Area 2 of Research
Naglis Malys, Dau-Yin Chang, R.G. Baumann, Dongmei Xie, and Lindsay W. Black* (2002)A bipartite bacteriophage T4 SOC and HOC randomized peptide display library: detection and analysis of phage T4 terminase (gp17) and late sigma factor (gp55) interaction. J. Mol. Biol. 319, 289-304.
Mullaney, J.M., Thompson, R.B., Gryczynski, Z.K. and Black, L.W. (2000). Green fluorescent protein as a probe of rotational mobility within bacteriophage T4. Journal of Virological Methods 88, 35-40.
Area 3 of Research
Catherine Bair, Dalin Rifat, and Lindsay Black (2005) Evolution of the Myoviridae internal protein I locus genes in response to host type IV modification dependent restriction enzymes, Phage/Virus Assembly, Colorado, 2005.
Faculty members:
Click here to update your contact information and create a profile.
|