I am a physical therapist by professional training. I worked as a clinician and physical therapy instructor from 1998-2002. I came to the University of Maryland School of Medicine in the Fall of 2002 to do a Ph.D. in Physical Rehabilitation Science. On completion of my Ph.D. in the Spring of 2008, I joined the Department of Physiology as a post doctoral fellow, and became a research associate in 2011.
My doctoral and post-doctoral work was aimed at developing a better understanding of how healthy and dystrophic muscles recover from contraction-induced skeletal muscle injuries. Towards the end of my doctoral training, I began to focus on muscle diseases linked to dysferlin, which is mutated in patients with LGMD2B and Miyoshi myopathy. My work suggests that the protein dysferlin, which was intially thought to repair damaged plasma membranes in skeletal muscle fibers, might actually play a more critical role in maintaining the integrity of internal membrane systems in skeletal muscle. My current research is focused on understanding the events that lead to myofiber death in the absence dysferlin by studying the response of muscle to in vivo injury.
Clinical Speciality:Exercise Physiology and Neuromuscular Disorders
Lab Techniques and Equipment:In vivo measurment of contractile function in skeletal muscle; In vivo protocols for experimental induction of muscle injury; Immunohistochemistry; Immunoblotting; Membrane fractionation and proteomics studies; Flow cytometry
Lack of correlation between outcomes of membrane repair assay and correction of dystrophic changes in experimental therapeutic strategy in dysferlinopathy. Lostal W, Bartoli M, Roudaut C, Bourg N, Krahn M, Pryadkina M, Borel P, Suel L, Roche JA, Stockholm D, Bloch RJ, Levy N, Bashir R, Richard I. PLoS One. 2012 (In Press). PMCID: PMC3362551
Distinct Effects of Contraction-induced Injury In Vivo on Four Different Murine Models of Dysferlinopathy. Roche JA, Ru LW, Bloch RJ. J Biomed Biotechnol. 2012;2012:134031. Epub 2012 Feb 6. PMCID: PMC3303924
Physiological and histological changes in skeletal muscle following in vivo gene transfer by electroporation. Roche JA, Ford-Speelman DL, Ru LW, Densmore AL, Roche R, Reed PW, Bloch RJ. Am J Physiol Cell Physiol. 2011 Nov;301(5):C1239-50. Epub 2011 Aug 10. PMCID: PMC3213910
Unmasking potential intracellular roles for dysferlin through improved immunolabeling methods. Roche JA, Ru LW, O'Neill AM, Resneck WG, Lovering RM, Bloch RJ. J Histochem Cytochem. J Histochem Cytochem. 2011 Nov;59(11):964-75. PMCID: PMC3261626
An in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery. Lovering RM, Roche JA, Goodall MH, Clark BB, McMillan A. J Vis Exp. 2011 May 11;(51).
Extensive mononuclear infiltration and myogenesis characterize the recovery of dysferlin-null skeletal muscle from contraction-induced injuries. Roche JA, Lovering RM, Roche R, Ru LW, Reed PW, Bloch RJ. Am J Physiol Cell Physiol. 2010 Feb;298(2):C298-312. PMCID: PMC2822489
Genetic manipulation of dysferlin expression in skeletal muscle: Novel insights into muscular dystrophy. Millay PD, Maillet M, Roche JA, Sargent MA, McNally EM, Bloch RJ, Molkentin JD. Am J Pathol. 2009 Nov;175(5):1817-23. Epub 2009 Oct 15. PMCID: PMC2774048
The rhoGEF Domain of Obscurin Activates rhoA Signaling in Skeletal Muscle. Ford-Speelman DL, Roche JA, Bowman AL, Bloch RJ. Mol Biol Cell. 2009 Sep;20(17):3905-17. Epub 2009 Jul 15. PMCID: PMC2735489
Gait analysis of locomotory impairment in rats before and after neuromuscular injury. Tang W, Lovering RM, Roche JA, Bloch RJ, Neerchal NK, Tasch U. J Neurosci Methods. Neurosci Methods. 2009 Jul 30;181(2):249-56. Epub 2009 May 9. PMCID: PMC3526839
Phospholipid-Induced In Vivo Particle Migration to Enhance Pulmonary Deposition. Ganguly S, Moolchandani V, Roche JA, Shapiro PS, Somaraju S, Eddington ND, Dalby RN. J Aerosol Med Pulm Drug Deliv. 2008 Dec;21(4):343-50.
Impaired recovery of dysferlin-null skeletal muscle after contraction-induced injury in vivo. Roche JA, Lovering RM, Bloch RJ. Neuroreport. 2008 Oct 29;19(16):1579-84. PMCID: PMC2662728
Identification of skeletal muscle mutations in tail snips from neonatal mice using immunohistochemistry. Lovering RM, O'Neill A, Roche JA, Bloch RJ. Biotechniques. 2007 Jun;42(6):702, 704.
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