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Luigi  Buonaguro
 

Luigi Buonaguro M.D.

Academic Title: Adjunct Associate Professor
Primary Appointment: Medicine
Additional Title(s): Senior Investigator, Mol. Biol. and Viral Oncogenesis, National Cancer Institute, Naples, Italy
lbuonaguro@ihv.umaryland.edu
Location: Istituto Nazionale Tumori "Fond. G. Pascale," Naples, Italy

Personal History:

Education

1988: Medical Doctor cum laude, Medical School, University of Naples, Italy.

1992: Board-Certified, Microbiology and Virology, Medical School, University of Naples, Italy.

Post Graduate Education and Training

1989: Internship at the Lab of Viral Oncology, National Cancer Institute "Fond. G. Pascale", Naples, Italy.

1990: Fellowship of the Italian Association on Cancer Research (A.I.R.C.) at the Lab. of Tumor Cell Biology, Natl. Cancer Inst., Natl. Institutes of Health, Bethesda, Maryland.

1991-1992: Fogarty Fellowship at the Lab. of Tumor Cell Biology, Natl. Cancer Inst., Natl. Institutes of Health, Bethesda, Maryland

1992-1993: Fellowship at the Lab of Viral Oncology, National Cancer Institute "Fond. G. Pascale", Naples, Italy.

1996: Visiting Scientist within the "International Exchange Program" of the National Research Council (CNR) at the Institute of Microbiology of the Regensburg University, Regensburg, Germany, (Director: Prof. Hans Wolf)

2004-2005: Visiting Scientist at the Institute of Human Virology, University of Maryland, Baltimore, Maryland (Director: Prof. R.C. Gallo)

2006 (Nov): Visiting Scientist at the Institute of Human Virology, University of Maryland, Baltimore, Maryland (Director Prof. R.C. Gallo).

Honors and Awards

1995-today: External expert of the "UNAIDS Network for isolation and characterization of HIV-1"

Research Interests:

1. Phylogenetic studies of HIV-1 isolates from Italy and endemic regions as preliminary studies for vaccine development.
2. Development of vaccine strategies, in particular anti-HIV-1 vaccine based on Virus-Like Particle (VLP) concept for B and non-B subtype variants.
3. Evaluation of immune-genomic patterns in response to different vaccine strategies.
4. Role of adjuvants in directing the immune response in vaccination protocols.

Publications:

Selected Publications

Buonaguro, L., Barillari, G., Chang, H.K., Bohan, C.A, Kao, V., Morgan, R., Gallo, R.C. and Ensoli, B.: Effects of the HIV-1 Tat protein on the expression of inflammatory cytokines. J. Virol. 66: 7156-7167, 1992.

Barillari, G., Buonaguro, L., Fiorelli, V., Hoffman, J., Michaels, F., Gallo, R.C. and Ensoli, B.: Effects of cytokines from activated immune cells on vascular cell growth and HIV-1 gene expression. J. Immunol. 149: 3727-3734, 1992.

Bohan, C.A., Kashanchi, F., Ensoli, B., Buonaguro, L., Boris-Lawrie, K. and Brady, J.: Analysis of Tat transactivation of Human Immunodeficiency Virus transcription in vitro. Genes Expression 2:391-407, 1992.

Ensoli, B., Buonaguro, L., Barillari, G., Fiorelli, V., Gendelman, R., Morgan, R.A., Wingfield, P. and Gallo, R.C.: Release, uptake and effects of extracellular HIV-1 Tat protein on cell growth nad viral transactivation. J. Virol. 67: 277-287, 1993.

Buonaguro, L., Buonaguro, F.M., Giraldo, G. and Ensoli, B.: The human immunodeficiency virus type 1 Tat protein transactivates Tumor Necrosis Factor ÃY gene expression through a TAR-like structure J. Virol. 68: 2677-2682, 1994.

Buonaguro, L., Greco, D., Tornesello, M.L., Rago, M., Zaccarelli, M., Curcio, F., Castello, G., Sguazzo, N., Pipolo, G., Beth-Giraldo, E., Buonaguro, F.M. and Giraldo, G.: Analysis of HIV-1 env Gene V3 Loop Sequence in a Southern Italian Cohort of Intravenous Drug Users. AIDS 8: 268-269, 1994.

Buonaguro, L., Del Gaudio, E., Monaco, M., Greco, D., Corti, P., Beth-Giraldo, E., Buonaguro, F.M., Giraldo, G. and the Italian-Ugandan Cooperation AIDS Program: Heteroduplex Mobility Assay and Phylogenetic Analysis of V3 Region Sequences of HIV-1 Isolates from Gulu-Northern Uganda. J. Virol. 69: 7971-7981, 1995.

Tornesello, M.L., Buonaguro, F.M., Meglio, A., Buonaguro, L., Beth-Giraldo, E., Giraldo, G.: Sequence variations and viral genomic state of Human papillomavirus type 16 in penile carcinomas from Ugandan patients. J. General Virology 78: 2199-2208, 1997.

Chang, H.K., Samaniego, F., Nair, B.C., Buonaguro, L., and Ensoli, B.: HIV-1 Tat protein exits from cells via a leaderless secretory pathway and binds to extracellular matrix-associated heparan sulfate proteoglycans through its basic region. AIDS 11: 1421-1431, 1997.

Buonaguro, L., Buonaguro, F.M., Russo, F., Tornesello, M.L., Beth-Giraldo, E., Wagner, R., Wolf, H., Giraldo, G.: A novel gp120 sequence from an HIV-1 isolate of the A clade identified in North Uganda. AIDS Res. Hum. Retroviruses 14: 1287-1289, 1998.

Tornesello, M.L., Buonaguro, F.M., Buonaguro, L., Salatiello, I., Beth-Giraldo, E., Giraldo, G.: Identification and functional analysis of sequence rearrangements in the long control region of human papillomavirus type 16 Af-1 variants isolated from Ugandan penile carcinomas. Journal of General Virology, 81: 2969-2982, 2000.

Buonaguro, F.M., Tornesello, M.L., Salatiello, I., Okong, P., Buonaguro, L., Beth-Giraldo, E., Biryahwaho, B., Sempala, S.D.K., Giraldo, G: The Uganda Study on HPV variants and genital cancers. J. Clin. Virol., 19: 31-41, 2000.

Buonaguro, L., Buonaguro, F.M., Tornesello, M.L., Mantas, D., Beth-Giraldo, E., Wagner, Michelson, S., Prevost, M.-C., Wolf, H., Giraldo, G.: High efficient production of Pr55gag Virus-like Particles expressing multiple HIV-1 epitopes, including a gp120 protein derived from an Ugandan HIV-1 isolate of subtype A. Antiviral Research, 49: 35-47, 2001

Buonaguro, L., Racioppi, L., Tornesello, M.L., Arra, C., Visciano, M.L., Biryahwaho, B., Sempala, S.D.K., Giraldo, G. and Buonaguro, F.M.: Induction of neutralizing antibodies and CTLs in Balb/c mice immunized with virus-like particles presenting a gp120 molecule from a HIV-1 isolate of clade A (HIV-VLPAs). Antiviral Research, 54: 189-201, 2002.

Buonaguro, L., Tagliamonte, M., Tornesello, M.L., Ciuffreda, D., Capiluppi, B., Lopalco, L., Lazzarin, A., Tambussi, G. and Buonaguro, F.M.: Molecular and phylogenetic characterization of HIV variants in Italian primari HIV infections (PHI): identification of non-B subtype variants. J. of Biological Regulators and Homeostatic Agents, 16: 44-48, 2002.

Tornesello, M.L., Duraturo, M.L., Salatiello, I., Buonaguro, L., Losito, S., Botti, G., Stellato, G., Greggi, S., Piccoli, R., Pilotti, E., Stefanon, B., De Palo, G., Franceschi, S. and Buonaguro, F.M.: Analysis of human papillomavirus type16 variants in Italian women with cervical intraepithelial neoplasia and cervical cancer. J. Med. Virology, 74: 117-126, 2004.

Buonaguro, L., Tagliamonte, M., Tornesello, M.L., Ciuffreda, D., Capiluppi, B., Lopalco, L., Lazzarin, A., Tambussi, G. and Buonaguro, F.M.: Screening of HIV-1 isolates by reverse Heteroduplex Mobility Assay and identification of non-B subtypes in Italy. J. AIDS, 37: 1295-1306, 2004.

Buonaguro, L., Tagliamonte, M., Tornesello, M.L. and Buonaguro, F.M.: Evaluation of a modified version of Heteroduplex Mobility Assay for rapid screening of HIV-1 isolates in epidemics characterized by mono/dual clade predominance. J. Virol. Methods, 124: 123-134, 2005.

Tornesello, M.L., Waddell, K.M., Duraturo, M.L., Biryahwaho, B., Downing R., Lucas S.B., Giani U., Buonaguro, L., and Buonaguro, F.M.: TP53 codon 72 polymorphism and risk of conjunctival squamous cell carcinoma in Uganda. Cancer Detect Prev. 29: 501-508, 2005.

Buonaguro, L., Visciano, M.L., Tornesello, M.L., Tagliamonte, M., Biryahwaho, B. and Buonaguro, F.M.: Induction of systemic and mucosal cross-clade neutralizing Antibodies in Balb/c mice immunized with HIV-1 clade A Virus-Like Particles administered by different routes of inoculation. J. Virol., 79: 7059-7067, 2005.

Arica, E., E. Wang, M.L. Tornesello, M. Tagliamonte, G.K. Lewis, F.M. Marincola, F.M. Buonaguro and L. Buonaguro.: Immature Monocyte Derived Dendritic Cells gene expression profile in response to Virus-Like Particles stimulation. J. Transl. Medicine 3:45, 2005.

Tornesello, M.L., Duraturo, M.L., Waddell, K.M., Biryahwaho, B., Downing R., Balinandi, S., Lucas S.B., Buonaguro, L., and Buonaguro, F.M.: Evaluating the role of human papillomaviruses in conjunctival neoplasia. Br J Cancer. 94: 446-449, 2006.

Buonaguro, L., Tornesello, M.L., Tagliamonte, M., Gallo, R.C., Wang, L.X., Kamin-Lewis, R., Abdelwahab, S., Lewis, G.K. and Buonaguro, F.M.: Baculovirus-derived Human Immunodeficiency virus type 1 Virus-Like Particles activate dendritic cells and induce ex vivo T-cell responses. J. Virol., 80: 9134-9142, 2006.
Tagliamonte, M., Vidal, N., Tornesello, M.L., Peeters, M., Buonaguro, F.M., and Buonaguro, L.: Genetic and phylogenetic characterization of structural genes from non-B HIV-1 subtypes in Italy. AIDS Res. Human Retroviruses, 22: 1045-1051, 2006.

Naderi, H.R., Tagliamonte, M., Tornesello, M.L., Ciccozzi, M., Rezza, G., Faridh, R., Buonaguro, F.M. and Buonaguro, L.: Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-Iran. Infectious Agents and Cancer, 1: 4, 2006.

Tornesello, M.L., Duraturo, M.L., Botti, G., Greggi, S., Piccoli, R., De Palo, G., Montella, M., Buonaguro, L., Buonaguro, F.M., Italian HPV Working Group: Prevalence of alpha-papillomavirus genotypes in cervical squamous intraepithelial lesions and invasive cervical carcinoma in the Italian population. J. Med. Virol., 78: 1663-1672, 2006

Tornesello, M.L., Duraturo, M.L, Buonaguro, L., Vallefuoco, G., Piccoli, R., Palmieri, S., and Buonaguro, F.M.: Prevalence of human papillomavirus genotypes and their variants in high risk West Africa women immigrants in South Italy. Infectious Agents and Cancer, 2: 1, 2007.

Buonaguro, L., Tagliamonte, M., Tornesello, M.L., and Buonaguro, F.M.: Evolution of the HIV-1 V3 region in the Italian epidemic. New Microbiol, 30: 1-11, 2007.

Buonaguro, L., Tagliamonte, M., Tornesello, M.L., and Buonaguro, F.M.: Genetic and phylogenetic evolution of HIV-1 in a low subtype heterogeneity epidemic: the Italian example. Retrovirology, 4: 34, 2007.

Buonaguro, L., Devito, C., Tornesello, M.L., Schroeder, U., Wahren, B., Hinkula, J., and Buonaguro, F.M.: DNA-VLP prime-boost intra-nasal immunization induces cellular and humoral anti-HIV-1 systemic and mucosal immunity with cross-clade neutralizing activity. Vaccine, 25: 5968-77, 2007.

Buonaguro, L., Tornesello, M.L., and Buonaguro, F.M.: HIV-1 subtype distribution in the worldwide epidemic: pathogenetic and therapeutic implications. J. Virol, 81:10209-19, 2007.

Tagliamonte, M., Naderi, H.R., Tornesello, M.L., Farid, R., Buonaguro, F.M., and Buonaguro L.: HIV Type 1 Subtype A Epidemic in Injecting Drug User (IDU) Communities in Iran. AIDS Res Hum Retroviruses, 23: 1569-1574, 2007.

Tornesello, M.L., Duraturo, M.L., Losito, S., Botti, G., Pilotti, S., Stefanon, B., De Palo, G., Gallo, A., Buonaguro, L., and Buonaguro, F.M.: Human papillomavirus genotypes and HPV16 variants in penile carcinoma. Int J Cancer, 122: 132-137, 2008.

Buonaguro, L., Monaco, A., Arica, E., Wang, E., Tornesello, M.L., Lewis, G.K., Marincola, F.M. and Buonaguro F.M.: Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation. BMC Bioinformatics 2008, 9(Suppl 2):S5

Tornesello, M.L., Duraturo, M.L., Sansone, M., Piccoli, R., Buonaguro, L., Buonaguro, F.M.: Human papillomavirus genotypes and HPV16 variants in Human Immunodeficiency Virus (HIV) positive Italian women. J Gen Virology 2008 Jun; [In Press]

Tornesello, M.L., Duraturo, M.L., Losito, S., Botti, G., Pilotti, S., Stefanon, B., De Palo, G., Gallo, A., Buonaguro, L., and Buonaguro, F.M.: Analysis of TP53 codon 72 polymorphism in HPV-positive and HPV-negative penile carcinoma. Cancer Letters 2008 [Accepted]


Links of Interest:

Institute of Human Virology