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Hervé  Tettelin

Hervé Tettelin Ph.D.

Academic Title: Associate Professor
Primary Appointment: Microbiology and Immunology
Additional Title(s): Associate Professor, Institute for Genome Sciences
Location: Biopark II, 629
Phone: (410) 706-6764
Fax: (410) 706-1482
Lab: (410) 706-6775

Personal History:

I received the degree of Industrial Engineer in Biotechnology from the Institut Supérieur Industriel de la Province de Liège, Belgium, and my B.S. and Ph.D. in Applied Natural Sciences from the Université Catholique de Louvain, Belgium. I was a postdoctoral fellow and a faculty member at the Institute for Genomic Research (TIGR) / J. Craig Venter Institute (JCVI) in Rockville, MD (1997-2007). I am currently affiliated with the Institute for Genome Sciences (IGS).

I have been in the genomics field for over 20 years, since the beginning of my B.Sc. in 1991. Over these two decades I have led the genome sequencing and analysis of many organisms, starting with eukaryotic chromosomes then shifting towards bacterial pathogens (over 300 genomes). I was the lead author on the first published genomes of Neisseria meningitidis , Streptococcus pneumoniae , and Streptococcus agalactiae. In collaboration with the group of Dr. Rino Rappuoli at Novartis Vaccines and Diagnostics (former Chiron Corporation), I pioneered the fields of reverse vaccinology and pan-genomics. The former makes use of genomics to identify novel protein candidates for vaccine development. The latter is the characterization of the genome content of an entire species using multiple strains. I have led recent developments of the Sybil software package that is aimed at the comparative genomic analysis of closely related bacterial genomes to understand their lifecycle, characterize their diversity, and identify novel candidates for vaccine development. In addition to genome sequencing, I have conducted many studies of bacterial diversity and transcriptional profiling using DNA microarrays and RNA-Seq.

Research Interests:

My general interests are the use of genomics, comparative genomics, and functional genomics to understand bacterial diversity and virulence, study host-pathogen interactions, and identify vaccine candidates and drug targets to cure disease.


Schoen C., Tettelin H., Parkhill J., and Frosch M. (2009) Genome flexibility in Neisseria meningitidis. Vaccine 27 supplement 2, B103-B111. Epub 2009 June 24.

Ishmael N., Dunning Hotopp J.C., Ioannidis P., Biber S., Sakamoto J., Siozios S., Nene V., Werren J., Bourtzis K., Bordenstein S.R., Tettelin H. (2009) Extensive Genomic Diversity of Closely Related Wolbachia Strains. Microbiology 155, 2211-2222. Epub 2009 Apr 23.

Bordenstein S.R., Paraskevopoulos C., Dunning Hotopp J.C., Sapountzis P., Lo N., Bandi C., Tettelin H., Werren J.H., and Bourtzis K. (2009) Parasitism and mutualism in Wolbachia: what the phylogenomic trees can and can not say. Mol. Biol. Evol. 26, 231-241. Epub 2008 Oct. 28.

Tettelin H. and Feldblyum T. (2009) Bacterial genome sequencing. In Methods in Molecular Biology 551: Molecular Epidemiology of Microorganisms, pp. 231-247, Caugant D.A. Ed., Humana Press Inc. (Clifton, NJ, USA).

Tettelin H. (2009) The bacterial pan-genome and reverse vaccinology. In Genome Dynamics 6: Microbial Pathogenomics, pp. 35-47, de Reuse H. and Bereswill S. Eds, Karger (Basel, Switzerland). DOI: 10.1159/000235761.

Kilian M., Poulsen K., Blomqvist T., Håvarstein L.S., Bek-Thomsen M., Tettelin H., and Sørensen U.B.S. (2008) Evolution of Streptococcus pneumoniae and its close commensal relatives. PLoS ONE 3(7), e2683, doi:10.1371/journal.pone.0002683. 

Tettelin H., Riley D., Cattuto C., and Medini D. (2008) Comparative genomics: the bacterial pan-genome. Curr. Opin. Microbiol. 11: 472-477 (doi:10.1016/j.mib.2008.09.006).

Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., and Holmes, D.S. (2008) Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications. BMC Genomics 9, 597, doi:10.1186/1471-2164-9-597. PMCID: PMC2621215.

Bek-Thomsen M., Tettelin H., Hance I., Nelson K.E., and Kilian M. (2008) Population diversity and dynamics of Streptococcus mitis, Streptococcus oralis and Streptococcus infantis in the upper respiratory tract of adults determined by a non-culture strategy. Infect. Immun., 76, 1889-1896. Epub 2008 Mar 3.

Jiang S.-M., Ishmael N., Dunning Hotopp J., Puliti M., Tissi 3., Kumar n., Cieslewicz M.J., Tettelin H., and Wessels M.R. (2008) Variation in the group B Streptococcus CsrRS regulon and effects on pathogenicity. J. Bacteriol. 190, 1956-1965. Epub 2008 Jan. 18.

Masignani V., Tettelin H., and Rappuoli R. (2007) Vaccines against infectious diseases: a biotechnology-driven evolution. In Genomic and Personalized Medicine, Willard H.F. and Ginsburg G.S. Eds, Elsevier/Academic Press (San Diego, CA, USA), vol. 1; pp: 562-570.

Raghavan N., Tettelin H., Miller A., Hostetler J., Tallon L., and Knight M. (2007) Nimbus (BgI): An active non-LTR retrotransposon of the Schistosoma mansoni snail host Biomphalaria glabrata. Int. J. Parasitol. 37, 1307-1318. Epub 2007 Apr. 19.

Dunning Hotopp J.C., Clark M.E., Oliveira D.C.S.G., Foster J.M., Fischer P., Mun~oz Torres M.C., Giebel J.D., Kumar N., Ishmael N., Wang S., Ingram J., Nene R.V., Shepard J., Tomkins J., Richards S., Spiro D.J., Ghedin E., Slatko B.E., Tettelin H.*, and Werren J. H.* [*These authors have contributed equally to this work] (2007) "Widespread lateral gene transfer from intracellular bacteria to multicellular eukaryotes." Science 317, 1753-1756. Epub 2007 Aug. 30.

Tettelin H., Rappuoli R., and Fraser-Liggett C.M. (2007) Using genomics to identify novel vaccine candidates against pathogens. In The Jordan Report, Accelerated Development of Vaccines 2007, U.S. Department of Health and Human Services, pp. 37-42.

Myers G.S.A., Parker D., Al-Hasani K., Kennan R.M., Seemann T., Ren Q., Badger J.H., Selengut J.D., DeBoy R.T., Tettelin H., Boyce J.D., McCarl V.P., Han X., Nelson W., Madupu R., Mohamoud Y., Holley T., Fedorova N., Khouri H., Bottomley S.P, Whittington R.J., Adler B., Songer J.G., Rood J.I., and Paulsen I.T. (2007) Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus. Nat. Biotechnol. 25, 569-575.

Lanie J.A., Ng W-L, Kazmierczak K.M., Andrzejewski T.M., Davidsen T.M., Wayne K.J., Tettelin H., Glass J.I., and Winkler M. (2006) Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6. J. Bacteriol. 189, 38-51.

Tettelin H., Medini D., Donati C., and Masignani V. (2006) Towards a universal group B Streptococcus vaccine using multi-strain genome analysis. Expert Rev. Vaccines 5, 687-694.

Dunning Hotopp J.C., Grifantini R., Kumar N., Tzeng Y.L., Fouts D., Frigimelica E., Draghi M., Giuliani M.M., Rappuoli R., Stephens D.S., Grandi G., and Tettelin H. (2006) Comparative genomics of Neisseria meningitidis: core genome, islands of horizontal transfer and pathogen specific genes. Microbiology 152, 3733-3749. Featured in Microbiology Today

Savard J., Tautz D., Richards S., Weinstock G.M., Gibbs R.A., Werren J.H., Tettelin H., and Lercher M.J. (2006) Phylogenomic analysis reveals bees and wasps (Hymenoptera) at the base of the radiation of holometabolous insects. Genome Res. 16, 1334-1338.

Baldo L., Dunning Hotopp J.C., Jolley K.A., Bordenstein S.R., Biber S.A., Choudhury R.R., Hayashi C., Maiden M.C.J., Tettelin H., and Werren J.H. (2006) A multilocus sequence typing system for the endosymbiont Wolbachia. Appl. Environ. Microbiol. 72, 7098-7110.

Ramaswamy S.V., Ferrieri P., Madoff L.C., Flores A.E., Kumar N., Tettelin H., and Paoletti L.C. (2006) Identification of novel cps locus polymorphisms in nontypeable group B Streptococcus. J. Med. Microbiol. 55, 775-783.

Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M.R., Khouri H., Zhang C., Hua N., Lin Q., Ohashi N., Zhi N., Nelson W., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., and Tettelin H. (2006) Comparative genomics of emerging human ehrlichiosis agents. PLoS Genet. 2, e21. Featured in Nature Reviews Microbiology

Medini D., Donati C., Tettelin H., Masignani V., and Rappuoli R. (2005) The microbial pan-genome. Curr. Opin. Genet. Dev. 15, 589-594.

Tettelin H., Masignani M., Cieslewicz M.J., Donati C., Medini D., Ward N.L., Angiuoli S.V., Crabtree J., Jones A., Durkin A.S., DeBoy R.T., Davidsen T.M., Mora M., Scarselli M., Margarit y Ros I., Peterson J.D., Hauser C.R., Sundaram J.P., Nelson W.C., Madupu R., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sullivan S.A., Daugherty S.C., Haft D.H., Selengut J., Gwinn M.L., Zhou L., Zafar N., Khouri H., Radune D., Dimitrov G., Watkins K., O'Connor K.J.B., Smith S., Utterback T.R., White O., Rubens C.E., Grandi G., Madoff L.C., Kasper D.L., Telford J.L., Wessels M.R., Rappuoli R., and Fraser C.M. (2005) Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial "pan-genome." Proc. Natl. Acad. Sci. USA 102, 13950-13955. Erratum in: Proc. Natl. Acad. Sci. USA 102, 16530. Featured in Nature Reviews Microbiology. Featured in The Scientist.

Maione D., Margarit I., Rinaudo C.D., Masignani V., Mora M., Scarselli M., Tettelin H., Brettoni C., Iacobini E.T., Rosini R., D'Agostino N., Miorin L., Buccato S., Mariani M., Galli G., Nogarotto R., Nardi Dei G., Vegni F., Fraser C.M., Mancuso G., Teti G., Madoff L.C., Paoletti L.C., Rappuoli R., Kasper D.L., Telford J.L., and Grandi G. (2005) Identification of a universal Group B Streptococcus vaccine by multiple genome screen. Science 309, 148-150. Featured in The Scientist.

Park I.K., Hsu A.P., Tettelin H., Shallom S., Drake S.K., Ding L., Wu U.I., Adamo N., Prevots D.R., Olivier K.N., Holland S.M., Sampaio E.P., and Zelazny A.M. (2015) Clonal diversification and changes in lipid traits and colony morphology in Mycobacterium abscessus clinical isolates. J. Clin. Microbiol. 3, 3438-3447. doi: 10.1128/JCM.02015-15.

Szymanski E.P., Leung J.M., Fowler C.J., Haney C., Hsu A.P., Chen F., Duggal P., Oler A.J., McCormack R., Podack E., Drummond R.A., Lionakis M.S., Browne S.K., Prevots D.R., Knowles M., Cutting G., Liu X., Devine S.E., Fraser C.M., Tettelin H., Olivier K.N., Holland S.M. (2015) Pulmonary nontuberculous mycobacterial infection: a multisystem multigenic disease. Am. J. Respir. Crit. Care Med. 192, 618-628 doi: 10.1164/rccm.201502-0387OC.

Tettelin H., Chancey S., Mitchell T., Denapaite D., Schähle Y., Rieger M., and Hakenbeck R. (2015) Genomics, genetic variation, and regions of differences. In Streptococcus pneumoniae: Molecular Mechanisms of Host-Pathogen Interactions, pp. 81-107, Brown J., Hammerschmidt S, and Orihuela C. Eds, Academic Press (San Diego, CA, USA).

Le Breton Y., Belew A.T., Valdes K.M., Islam E., Curry P., Tettelin H., Shirtliff M.E., El-Sayed N.M., and McIver K.S. (2015) Essential genes in the core genome of the human pathogen Streptococcus pyogenes. Sci. Rep. 5, 9838. doi: 10.1038/srep09838.

Rosini R., Campisi E., De Chiara M., Tettelin H., Rinaudo D., Toniolo C., Metruccio M., Guidotti S., Skov Sørensen U.B., Kilian M., DEVANI Consortium, Ramirez M., Janulczyk R., Donati C., Grandi G., and Margarit I. (2015) Genomic analysis reveals the molecular basis for capsule loss in group B Streptococcus population. PLoS One 10, e0125985. doi:10.1371/journal.pone.0125985.

Chancey S.T., Agrawal S., Schroeder M.R., Farley M.M., Tettelin H.*, and Stephens D.S.* [*These authors have contributed equally to this work] (2015) Composite mobile genetic elements disseminating macrolide resistance in Streptococcus pneumoniae. Front. Microbiol. 6, 26. doi: 10.3389/fmicb.2015.00026.

Chancey S.T., Bai X., Kumar N., Drabek E.F., Daugherty S.C., Colon T., Ott S., Sengamalay N., Sadzewicz L., Tallon L.J., Fraser C.M., Tettelin H. and Stephens D.S. (2015) Transcriptional attenuation controls macrolide inducible efflux and resistance in Streptococcus pneumoniae and in other Gram-positive bacteriacontaining mef/mel(msr(D))elements. PLoS One 10, e0116254. doi: 10.1371/journal.pone.0116254.

Hooven T.A., Randis T.M., Daugherty S.C., Narechania A., Planet P.J., Tettelin H.*, and Ratner A.J.* [*These authors have contributed equally to this work] (2014) Complete genome sequence of Streptococcus agalactiae strain CNTC 10/84, a hypervirulent sequence type 26 strain. Genome Announc. 2, e01338-14. doi:10.1128/genomeA.01338-14.

Vernikos G., Medini D., Riley D.R., and Tettelin H. (2015) Ten years of pan-genome analyses. Curr. Opin. Microbiol. 23, 148-154.

Bessen D.E., McShan W.M., Nguyen S.V., Shetty A., Agrawal S., and Tettelin H. (2014) Molecular epidemiology and genomics of group A Streptococcus. Infect. Genet. Evol. (MEEGID). pii: S1567-1348(14)00382-7. doi: 10.1016/j.meegid.2014.10.011.

Kilian M., Riley D.R., Jensen A., Bru¨ggemann H., and Tettelin H. (2014) Parallel evolution of Streptococcus pneumoniae and Streptococcus mitis to pathogenic and mutualistic lifestyles. mBio 5, e01490-14. doi:10.1128/mBio.01490-14.

Dugan V.G., Emrich S.J., Giraldo-Calderón G.I., Harb O.S., Newman R.M., Pickett B.E., Schriml L.M., Stockwell T.B., Stoeckert C.J. Jr, Sullivan D.E., Singh I., Ward D.V., Yao A., Zheng J., Barrett T., Birren B., Brinkac L., Bruno V.M., Caler E., Chapman S., Collins F.H., Cuomo C.A., Di Francesco V., Durkin S., Eppinger M., Feldgarden M., Fraser C.M., Fricke W.F., Giovanni M., Henn M.R., Hine E., Dunning Hotopp J., Karsch-Mizrachi I., Kissinger J.C., Lee E.M., Mathur P., Mongodin E.F., Murphy C.I., Myers G., Neafsey D.E., Nelson K.E., Nierman W.C., Puzak J., Rasko D., Roos D.S., Sadzewicz L., Silva J.C., Sobral B., Squires R.B., Stevens R.L., Tallon L., Tettelin H., Wentworth D., White O., Will R., Wortman J., Zhang Y., Scheuermann R.H. (2014) Standardized metadata for human pathogen/vector genomic sequences. PLoS One 9, e99979. doi: 10.1371/journal.pone.0099979.

Tettelin H. (2004) Streptococcal genomes provide food for thought (News and Views). Nat. Biotechnol. 22, 1523-1524.

Ward N., Larsen Ø., Sakwa J., Bruseth L., Khouri H., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K., Lewis M., Nelson K.E., Methé B., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D., Ravel J., Tettelin H., Ren Q., Read T., DeBoy R., Seshadri R., Salzberg S.L., Jensen H.B., Kåre Birkeland N., Nelson W., Dodson R.J., Grindhaug S.H., Holt I., Eidhammer I., Jonasen I., Vanaken S., Utterback T., Feldblyum T.V., Lillehaug J., Fraser C.M., and Eisen J.A. (2004) Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath). PLoS Biol. 2, 1616-1628 [2(10): e303].

Tettelin H. and Parkhill J. (2004) The use of genome annotation data and its impact on biological conclusions (correspondence letter). Nature Genetics 36, 1028-1029.

Nierman W., DeShazer D., Kim H., Tettelin H., Nelson K, Feldblyum T., Ulrich R., Ronning C., Brinkac L., Daugherty S., Davidsen T., DeBoy R., Dimitrov G., Dodson R., Durkin S., Gwinn M., Haft D., Khouri H., Kolonay J., Madupu R., Mohammoud Y., Nelson W., Radune D., Romero C., Sarria S., Selengut J., Shamblin C., Sullivan S., White O., Yu Y., Zafar N., Zhou L., and Fraser C.M. (2004) Structural Flexibility in the Burkholderia mallei Genome. Proc. Natl. Acad. Sci. USA 101, 14246-14251.

Tettelin H. and Hollingshead S.K. (2004) Comparative genomics of S. pneumoniae: intra-strain diversity and genome plasticity. In The pneumococcus, pp. 15-29, Tuomanen E., Mitchell T., Morrison D. and Spratt B. Eds. ASM Press (Washington, DC, USA).

Seshadri R., Myers G.S.A., Tettelin H., Eisen J.A., Heidelberg J.F., Davidsen T.M., Dodson R.J., Deboy R.T., Fouts D.E., Haft D.H., Selengut J., Ren Q., Brinkac L.M., Madupu R., Kolonay J.F., Durkin A.S., Daugherty S.C., Shetty J., Shvartsbeyn A., Gebregeorgis E., Geer K., Tsegaye G., Malek J., Ayodeji B., Shatsman S., McLeod M.P., Majs D., Howell J.K., Pal S., Amin A., Vashisth P., McNeill T.Z., Weinstock G.M., Norris S.J., Fraser C.M., and Paulsen I.T. (2004) Comparison of the genome of the oral pathogen, Treponema denticola, with other spirochete genomes. Proc. Natl. Acad. Sci. USA 101, 5646-5651.

Wu M., Sun L.V., Vamathevan J., Riegler M., DeBoy R., Brownlie J.C., McGraw E.A., Martin W., Esser C., Ahmadinejad N., Wiegand C., Madupu R., Beanan M.J., Brinkac L.M., Daugherty S.C., Durkin A.S., Kolonay J.F., Nelson W.C., Mohamoud Y., Lee P., Berry K., Young M.B., Utterback T., Weidman J., Nierman W.C., Paulsen I.T., Nelson K.E., Tettelin H., O'Neill S.L., and Eisen J.A. (2004) Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. PLoS Biol. 2: 327-341.

Radune D. and Tettelin H. (2004) Optimized multiplex polymerase chain reaction, an effective method for rapid gap closure. In Methods in Molecular Biology 255: Bacterial Artificial Chromosomes, pp. 309-318, Zhao S. and Stodolsky M. Eds, Humana Press Inc. (Totowa, NJ, USA).

Tettelin H. and Feldblyum T. (2004) Genome sequencing and analysis. In Genomics, Proteomics and Vaccines, pp. 45-73, Grandi G. Ed., John Wiley and Sons Ltd (London, United Kingdom).

Telford J.L., Margarit y Ros I., Maione D., Masignani V., Tettelin H., Bensi G., and Grandi G. (2004) Vaccines against pathogenic streptococci. In Genomics, Proteomics and Vaccines, pp. 205-222, Grandi G. Ed., John Wiley and Sons Ltd (London, United Kingdom).

Nelson K.E., Fleischmann R.D., DeBoy R.T., Paulsen I.T., Fouts D.E., Eisen J.A., Daugherty S.C., Dodson R.J., Durkin A.S., Gwinn M., Haft D.H., Kolonay J.F., Nelson W.C., Mason T., Tallon L., Gray J., Granger D., Tettelin H., Dong H., Galvin J.L., Duncan M.J., Dewhirst F.E., and Fraser C.M. (2003) Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83. J. Bacteriology 185, 5591-5601.

Read T.D., Peterson S.N., Tourasse N., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Økstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M., DeBoy R.T., Madupu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstø A-B., and Fraser C.M. (2003) The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature 423, 81-86.

Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., Deboy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A., Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., and Heidelberg J.F. (2003) Complete genome sequence of the Q-fever pathogen Coxiella burnetii. Proc. Natl. Acad. Sci. USA 100, 5455-5460.

Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L., Brinkac L., Beanan M., Daugherty S., DeBoy R.T., Durkin S., Kolonay J., Madupu R., Nelson W., Vamathevan J., Tran B., Upton J., Hansen T., Shetty J., Khouri H., Utterback T., Radune D., Ketchum K.A., Dougherty B.A., and Fraser C.M. (2003) Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science 299, 2071-2074.

Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L., Brinkac L.M., Beanan M.J., Daugherty S.C., Deboy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., and Fraser C.M. (2002) The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. Proc. Natl. Acad. Sci. USA 99: 13148-13153.

Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S., Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D., Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Lewis M.R., Radune D., Fedorova N.B., Scanlan D., Khouri H., Mulligan S., Carty H.A., Cline R.T., Van Aken S.E., Gill J., Scarselli M., Mora M., Iacobini E.T., Brettoni C., Galli G., Mariani M., Vegni F., Maione D., Rinaudo D., Rappuoli R., Telford J.L., Kasper D.L., Grandi G., and Fraser C.M. (2002) Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. Proc. Natl. Acad. Sci. USA 99: 12391-12396.

Grifantini R., Bartolini E., Muzzi A., Draghi M., Frigimelica E., Berger J., Ratti G., Petracca R., Galli G., Agnusdei M., Giuliani M.M., Santini L., Brunelli B., Tettelin H., Rappuoli R., Randazzo F., and Grandi G. (2002) Previously unrecognized vaccine candidates against Group B meningococcus identified by DNA microarrays. Nat. Biotechnol. 20: 914-921.

Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.L., Yang F., Holt I., Umayam L.A., Mason T., Brenner M., Shea T.P., Parksey D., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J., Khouri H., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., and Fraser C.M. (2002) The complete genome sequence of Cholorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium. Proc. Natl. Acad. Sci. USA 90, 9509-9514.

Read T.D., Gill S.R., Tettelin H., and Dougherty B.A. (2001) Finding drug targets in microbial genomes. Drug Discovery Today 6, 887-892.

Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S., Heidelberg J., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D., Holtzapple E., Khouri H., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., and Fraser C.M. (2001) Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science 293, 498-506. Fast Breaking Paper. Hot paper 1. Hot paper 2.

Masignani V., Giuliani M.M., Tettelin H., Commanducci M., Rappuoli R., and Scarlato V. (2001) A Mu-like prophage in serogroup B Neisseria meningitidis coding for surface-exposed antigens. Infection and Immunity 69, 2580-2588.

Peterson S., Cline R.T., Tettelin H., Sharov V., and Morrison D.A. (2000) Gene expression analysis of the Streptococcus pneumoniae competence regulons by use DNA microarrays. J. Bacteriology 182, 6192-6202.

Saunders N.J., Jeffries A.C., Peden J.F., Hood D.W., Tettelin H., Rappuoli R., and Moxon E.R. (2000) Repeat-associated phase variable genes in the complete genome sequence of Neisseria meningitidis strain MC58. Mol. Microbiol. 37, 207-215.

Heidelberg J., Eisen J.A., Nelson W.C., Clayton R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D., Ermolaeva M.D., Vamathevan J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L., Utterback T., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J., Venter J.C., and Fraser C.M. (2000) DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406, 477-484.

Tettelin H., Saunders N.J., Heidelberg J., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H., Qin H., Vamathevan J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., and Venter J.C. (2000) Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science 287, 1809-1815. Hot Paper in The Scientist

Pizza M., Scarlato V., Masignani V., Giuliani M.M., Arica B., Comanducci M., Jennings G.T., Baldi L., Bartolini E., Capecchi B., Galeotti C.L., Luzzi E., Manetti R., Marchetti E., Mora M., Nuti S., Ratti G., Santini L., Savino S., Scarselli M., Storni E., Zuo P., Broeker M., Hundt E., Knapp B., Blair E., Mason T., Tettelin H., Hood D.W., Jeffries A.C., Saunders N.J., Granoff D.M., Venter J.C., Moxon E.R., Grandi G., and Rappuoli R. (2000) Whole genome sequencing to identify vaccine candidates against serogroup B meningococcus. Science 287, 1816-1820.

Tettelin H., Davidson R.M., Agrawal S., Aitken M., Shallom S., Hasan N.A., Strong M., Calado Nogueira de Moura V., De Groote M.A., Duarte R.S., Hine E., Parankush S., Su Q., Daugherty S.C., Fraser C.M., Brown-Elliott B.A., Wallace Jr. R.J., Holland S.M., Sampaio E.P., Olivier K.N., Jackson M., and Zelazny A.M. (2014) High-level relatedness among Mycobacterium abscessus subsp. massiliense strains from widely separated outbreaks. Emerg. Infect. Dis. 20, 364-371

Zubair S., de Villiers E.P., Younan M., Andersson G., Tettelin H., Riley D.R., Jores J., Bongcam-Rudloff E., and Bishop R.P. (2013) Genome sequences of two pathogenic Streptococcus agalactiae isolates from the one-humped camel Camelus dromedarius. Genome Announc. 1, e00515-13.

Shallom S.J., Gardina P.J., Myers T.G., Sebastian Y., Conville P., Calhoun L.B., Tettelin H., Olivier K.N., Uzel G., Sampaio E.P., Holland S.M., and Zelazny A.M. (2013) New rapid scheme for distinguishing subspecies of the Mycobacterium abscessus group and identification of Mycobacterium massiliense with inducible clarithromycin resistance. J. Clin. Microbiol. 51, 2943-2949. Epub 2013 Jun 26.

Kimaro Mlacha S.Z., Romero-Steiner S., Dunning Hotopp J.C., Kumar N., Ishmael N., Riley D.R., Farooq U., Creasy T.H., Tallon L.J., Liu X., Goldsmith C.S., Sampson J., Carlone G.M., Hollingshead S.K., Scott J.A.G., and Tettelin H. (2013) Phenotypic, genomic, and transcriptional characterization of Streptococcus pneumoniae interacting with human pharyngeal cells. BMC Genomics 14, 383.

Kimaro Mlacha S.Z., Peret T.C., Kumar N., Romero-Steiner S., Dunning Hotopp J.C., Ishmael N., Grinblat-Huse V., Riley D.R., Erdman D.D., Carlone G.M., Sampson J., Scott J.A.G., and Tettelin H. (2013) Transcriptional adaptation of pneumococci and human pharyngeal cells in the presence of a virus infection. BMC Genomics 14, 378.

Klinzing D.C., Ishmael N., Dunning Hotopp J.C., Tettelin H., Shields K.R., Madoff L.C., and Puopolo K.M. (2013) The two-component response regulator LiaR regulates cell wall stress responses, pili expression, and virulence in group B Streptococcus. Microbiology 159,1521-1534. Epub 2013 May 23.

Le Breton Y., Mistry P., Valdes K., Quigley J., Kumar N., Tettelin H., and McIver K. (2013) Genome-wide identification of genes required for fitness of the group A Streptococcus in human blood. Infect. Immun. 81, 862-875. Epub 2013 Jan 7.

Tettelin H., Sampaio E.P., Daugherty S., Hine E., Riley D.R., Sadzewicz L., Sengamalay N., Shefchek K., Su Q., Tallon L.J., Conville P., Olivier K., Holland S.M., Fraser C.M., and Zelazny A.M. (2012) Genomic insights into the emerging human pathogen Mycobacterium massiliense. J. Bacteriol. (Genome Announcement) 194, 5450.

Krauland M.G., Dunning Hotopp J.C., Riley D.R., Daugherty S.C., Marsh J.W., Messonier N.E., Mayer L.W., Tettelin H., and Harrison L.H. (2012) Whole genome sequencing to investigate the emergence of clonal complex 23 Neisseria meningitidis serogroup Y disease in the United States. PLoS One 7, e35699. Epub 2012 Apr 27.

Brüggemann H., Lomholt H.B., Tettelin H., and Kilian M. (2012) CRISPR/cas loci of type II Propionibacterium acnes confer immunity against acquisition of mobile elements present in type I P. acnes. PLoS One 7, e34171. Epub 2012 Mar 30.

Hiller N.L., Eutsey R.A., Powell E., Earl J., Janto B., Martin D., Dawid S., Ahmed A., Longwell M., Dahlgreen M., Ezzo S., Tettelin H., Daugherty S.C., Mitchell T., Hillman T., Buchinsky F., Tomasz A., de Lencastre H., Sá-Leão R., Post J.C., Hu F.Z., and Ehrlich G.D. (2011) Differences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone. PLoS One 6, e28850. Epub 2011 Dec 19.

Sanchez C.J., Kumar N., Lizcano A., Shivshankar P., Dunning Hotopp J.C., Jorgensen J.H., Tettelin H., and Orihuela C.J. (2011) Streptococcus pneumoniae in biofilms are unable to cause invasive disease due to altered virulence determinant production. PLoS One 6, e28738. Epub 2011 Dec 8.

Riley D.R., Angiuoli S.V., Crabtree J., Dunning Hotopp J.C., and Tettelin H. (2011) Using Sybil for interactive comparative genomics of microbes on the web. Bioinformatics 28, 160-166. Epub 2011 Nov 24.

Bessen D.E., Kumar N., Hall G.S., Riley D.R., Luo F., Lizano S., Ford C.N., McShan W.M., Nguyen S.V., Dunning Hotopp J.C., and Tettelin H. (2011) Whole genome association study on tissue tropism phenotypes in group A Streptococcus. J. Bacteriol. 193, 6651-6663. Epub 2011 Sep 23.

Lee A.W., Tettelin H., and Chancey S. (2011) Genomic analyses of clonal isolates provide clues to the evolution of Streptococcus pneumoniae (Commentary). Front. Microbio. 2, 63.

Angiuoli S.V., Dunning Hotopp J.C., Salzberg S.L., and Tettelin H. (2011) Improving pan-genome annotation using whole genome multiple alignment. BMC Bioinformatics 12, 272.

Budroni S., Siena E., Dunning Hotopp J.C., Seib K.L., Serruto D., Nofroni C., Comanducci M., Riley D.R., Daugherty S.C., Angiuoli S.V., Covacci A., Pizza M., Rappuoli R., Moxon R., Tettelin H.*, and Medini D.* [*These authors have contributed equally to this work] (2011) Neisseria meningitidis is structured in clades associated with restriction modification systems that modulate homologous recombination. Proc. Natl. Acad. Sci. USA 108, 4494-4499. Epub 2011 Feb 28.

Donati C., Hiller N.L., Tettelin H., Muzzi A., Croucher N.J., Angiuoli S.V., Oggioni M., Dunning Hotopp J.C., Hu F.Z., Riley D., Covacci A., Mitchell T.J., Bentley S.D., Kilian M., Ehrlich G.D., Rappuoli R., Moxon E.R., and Masignani V. (2010) Structure and dynamics of the pan-genome of Streptococcus pneumoniae and closely related species. Genome Biol. 11, R107.

Bottacini F., Medini D., Pavesi A., Turroni F., Foroni E., Riley D., Giubellini D., Tettelin H., van Sinderen D., and Ventura M. (2010) Comparative genomics of the genus Bifidobacterium. Microbiology 156, 3243-3254. Epub 2010 Jul 15.

Janulczyk R., Masignani V., Maione D., Tettelin H., Grandi G., and Telford J. (2010) Simple sequence repeats and genome plasticity in Streptococcus agalactiae. J. Bacteriol. 192, 3990-4000. Epub 2010 May 21.

Gomes Moriel D., Bertoldi I., Spagnuolo A., Marchi S., Rosini R., Nesta B., Pastorello I., Mariani Corea V.A., Torricelli G., Cartocci E., Savino S., Scarselli M., Dobrindt U., Hacker J., Tettelin H., Tallon L.J., Sullivan S., Wieler L.H., Ewerse C., Pickard D., Dougan G., Rita Fontana M., Rappuoli R., Pizza M., and Serino L. (2010) Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli. Proc. Natl. Acad. Sci. USA 107, 9072-9077. Epub 2010 May 3.

van Schaik W., Top J., Riley D.R., Boekhorst J., Vrijenhoek J.E., Schapendonk C.M., Hendrickx A.P., Nijman I.J., Bonten M.J., Tettelin H., and Willems R.J. (2010) Pyrosequencing-based comparative genome analysis of the nosocomial pathogen Enterococcus faecium and identification of a large transferable pathogenicity island. BMC Genomics 11, 239. Epub 2010 Apr 14.

Tettelin H., Radune D., Kasif S., Khouri H., and Salzberg S.L. (1999) Optimized multiplex PCR: efficiently closing a whole genome shotgun sequencing project. Genomics 62, 500-507.

Salzberg S.L., Pertea M., Delcher A.L., Gardner M.J., and Tettelin H. (1999) Interpolated markov models for eukaryotic gene finding. Genomics 59, 24-31.

Gardner M.J., Tettelin H., Carucci D.J., Cummings L.M., Smith H.O., Fraser C.M., Venter J.C., and Hoffman S.L. (1999) The malaria genome sequencing project: complete sequence of P. falciparum chromosome 2. Parassitologia 41, 69-75.

Jing J., Lai Z., Aston C., Lin J., Carucci D.J., Gardner M.J., Mishra B., Anantharaman T.S., Tettelin H., Cummings L.M., Hoffman S.L., Venter J.C., and Schwartz D.C. (1999) Optical mapping of Plasmodium falciparum chromosome 2. Genome Research 9, 175-181.

Gardner M.J., Tettelin H., Carucci D.J., Cummings L.M., Aravind L., Koonin E.V., Shallom S., Mason T., Yu K., Fujii C., Pedersen J., Shen K., Jing J., Aston C., Lai Z., Schwartz D.C., Pertea M., Salzberg S., Zhou L., Sutton G.G., Clayton R.L., White O., Smith H.O., Fraser C.M., Adams M.D., Venter J.C., and Hoffman S.L. (1998) Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. Science 282, 1126-1132.

Carucci D.J., Gardner M.J., Tettelin H., Cummings L.M., Smith H.O., Adams M.D., Venter J.C., and Hoffman S.L. (1998) Sequencing the genome of Plasmodium falciparum. Current Opinion in Infectious Diseases 11, 531-534.

Carucci D.J., Gardner M.J., Tettelin H., Cummings L.M., Smith H.O., Adams M.D., Hoffman S.L., and Venter J.C. (1998) The malaria genome sequencing project. Expert Rev. Mol. Med. 1998, 1-9. Online Journal

Gardner M.J., Tettelin H., Carucci D.J., Cummings L.M., Adams M.D., Smith H.O., Venter J.C., and Hoffman S.L. (1998) The malaria genome sequencing project. Protist 149, 109-112.

Tettelin H., Thierry A., Goffeau A., and Dujon B. (1998) Physical mapping of chromosomes VII and XV of Saccharomyces cerevisiae at 3.5 kb average resolution to allow their complete sequencing. Yeast 14, 601-616.

Caro L.H.P., Tettelin H., Vossen J.H., Ram A.F.J., van den Ende H., and Klis F.M. (1997) In silicio identification of glycosyl-phosphatidylinositol-anchored plasma membrane and cell wall proteins of Saccharomyces cerevisiae. Yeast 13, 1477-1489.

Tettelin H., ..., and Kleine K. (115 authors) (1997) The nucleotide sequence of Saccharomyces cerevisiae chromosome VII. Nature 387 supplement, 81-84.

Dujon B., ..., Tettelin H., ..., and Kleine K. (97 authors) (1997) The nucleotide sequence of Saccharomyces cerevisiae chromosome XV. Nature 387 supplement, 98-102.

Bussey H., ..., Tettelin H., ..., and Hani J. (124 authors) (1997) The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI. Nature 387 supplement, 103-105.

Van Dyck L., Tettelin H., Purnelle B., and Goffeau A. (1997) An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthetase, remnants of Ty and three tRNA genes. Yeast 13, 171-176.

Goffeau A., Barrell B.G., Bussey H., Davis R.W., Dujon B., Feldmann H., Galibert F., Hoheisel J.D., Jacq C., Johnston M., Louis E.J., Mewes H.W., Murakami Y, Philippsen P., Tettelin H., and Oliver S.G. (1996) Life with 6000 genes. Science 274, 546-567.

Tettelin H., Thierry A., Fairhead C., Perrin A., and Dujon B. (1995) In vitro fragmentation of yeast chromosomes and yeast artificial chromosomes at artificially inserted sites and applications to genome mapping. In Methods in Molecular Genetics 6, pp. 81-107. Adolph K.W. Ed., Academic Press.

Dujon B., ..., Tettelin H., ..., and Mewes H.W. (108 authors) (1994) Complete DNA sequence of yeast chromosome XI. Nature 369, 371-378.

Purnelle B., Tettelin H., Van Dyck L., Skala J., and Goffeau A. (1993) The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames. Yeast 9, 1379-1384.